A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme PstI, which cuts the plasmid at a single site in the ampicillin-resistance gene. The DNA is then annealed with a PstI digest of human DNA, ligated, and used to transform E. coli cells. What antibiotic-resistance phenotype(s) (characteristics) will be found on the plate

Answers

Answer 1

Answer: Tetracycline.

Explanation:

A plasmid is a small circular DNA molecule found in bacteria that is separate from the bacterial chromosome and replicates independently of it. They encode for certain genes that play a key role in antibiotic resistance. Restriction enzymes are endonucleases that catalyze the cleavage of phosphodiester bonds in different regions located within a DNA strand. PstI is an example of an endonuclease, it is a type II restriction enzyme produced by the microorganism Providencia stuartii that possesses a restriction target in double-stranded DNA dependent on an unmethylated, palindromic, asymmetric sequence, and in this example, it cuts the plasmid at a single site in the ampicillin-resistance gene. After that, the DNA is annealed with another fragment of DNA and this new recombinant molecule is used to transform E. coli cells. Transformation is a key step in DNA cloning because it occurs after restriction enzyme digestion and ligation treatments and transfers newly made plasmids into bacteria. So these bacterias will have a new fragment of DNA, which still has a tetracycline resistance gene but it no longer has the ampicillin resistance gene because it was disrupted by the restiction enzyme. Thereby, the antibiotic resistance phenotype is the tetracycline resistace.


Related Questions

A community ecologist would most likely study A. population growth of a single species. B. interactions among individuals of different species living in a small area. C. the cycling of matter through biotic and abiotic components of a geographical area. D. interactions among individuals of the same species living in a small area. E. energy flow through an ecosystem.

Answers

Answer:

The correct answer is B. interactions among individuals of different species living in a small area.

Explanation:

A community ecologist studies the set of populations of organisms of different species that inhabit and interact with each other in the same place for a period of time. The ecological community has to be studied as a unit or set and not independently each species of living beings that forms and interacts, that is, their distribution, organization, quantity, demography and reciprocity between the different populations that cohabit are included. Likewise, the main perspective of a  community ecologist is found in the interrelationships between populations depending on the genotypic and phenotypic characteristics determined.

What is an oxygen debt? How is it repaid? Explain in detail.

Answers

Answer:

oxygen debt a state that arises in very active muscles when insufficient oxygen is supplied by the lungs, causing the muscle tissue to respire anaerobically with the production of LACTIC ACID. When muscular activities slow down, the rate and depth of breathing remains at a high level until the lactic acid has been oxidized. an oxygen debt can be repayed by breathing heavily

Explanation:

3 Stimuli Plants that adapt to

Answers

Water, gravity, and light.

Answer:

-light

-gravity

-water

Which statement is true?

A. Temperatures on the Moon are always the same as the temperatures at the Earth’s poles.

B. Temperatures on the Moon are always hotter than the Earth.

C. Temperatures on the Moon are always colder than the Earth.

D. Temperatures on the Moon fall into a wider range than on the Earth.

Answers

D

Because the moon lacks an atmosphere and it’s day night cycle is so long, one side of the moon will spend a long time in the suns rays with no atmosphere to absorb the energy, while the other side will have no sunlight at all. The lit side will reach extremely high temperatures, and the dark side will reach extremely cold ones.

suggests that children are active learners who essentially socialize
themselves.
1) Gender schema theory
2) The autobiographical author
3) Theory of mind
4) Social learning theory

Answers

Answer: gender schema theory

Explanation:

Suggest that children are active learners who essentially socialize themselves by Gender schema theory.

What do you mean by Gender schema theory?

Gender schema theory may be defined as a concept that children understand regarding male and female positions and roles from the culture in which they live.

Gender schema theory deals with the principle of socialization among young children. It is the basis of learning the concept and functions associated with societies.

Therefore, suggest that children are active learners who essentially socialize themselves by Gender schema theory.

To learn more about Gender schema theory, refer to the link:

https://brainly.com/question/14314047

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HELP EASY QUESTION Definition: This is an organism's response to a stimulus developed by being taught or watching another organism. Example: how to open a soda when you're thirsty

Answers

It is a learned behavior.

26. the first documented patient in a disease epidemic within a population?
A. The suspect
B. The definitive host
C. The carrier
D. The index case

27. During the lab, the time was needed for E. Coli to show some growth after UV light exposure which indicates some DNA repair?
A. 0 second
B. 30 seconds
C. 1 minute
D. 2 minutes

28. Mannitol salt agar is classified as:
A. Enriched medium
B. Selective medium
C. Differential medium
D. A. and b.
E. B. and c.

29. Mannitol salt agar is used to identify & differentiate
A. Fungi
B. Enteric bacteria
C. Staphylococcus
D. Streptococcus
E. Gram (-) cocci

30. The Ingredient in Mannitol salt plate which makes the agar selective is?
A. Salt
B. Glucose
C. Mannitol
D. Bile acids

Answers

Answer:

a a c

Explanation:

What do well sorted settlements have

Answers

Answer:

Explanation:

Well-sorted sediments have grains of similar size, and are the result of much sediment transport and earth processes which segregated the sediment by depositing different size fractions in different places.

Hope this helped!!!

Michael takes a personal inventory of the food he eats. Which foods on his inventory could be classified as nutritious? Check all that apply.

a bowl of fruit
steamed vegetables
a bag of potato chips
a candy bar
whole wheat toast
fried onion rings
a grilled chicken breast

Answers

Answer:

a bowl of fruit

steamed vegetables

whole wheat toast

a grilled chicken breast

Explanation:

Just answered the question on Edge and got it correct.

Quick question. I am trying to cite my sources for my biology research project and the website that I used is a rare disease informational center with different topics. For example there is a summary section, a symptoms section etc.... I need to cite my sources however each section has a different publication date because they don’t update the entire article at once. I am currently trying to use an in text citation for the summary because I used a quote from the summary so do I just use the summary publication? But I did use other sections of this website for other parts of my essay so when it comes time to making my bibliography what do I do

Answers

Answer:

For your bibliography, you need to put the website but also put the sections you got your examples from. Also, don't forget to put the person who published the summary down as well. Hope this helped

Explanation:

I may be in high school but from time to time I'm in the same situation at school when it comes to things like this.

When the blood glucose level decreases during a fast or between meals, the pancreas releases glucagon. Glucagon signals the liver to stop consuming glucose and to start producing glucose. Select the statements that describe how glucagon activates glucose production in the liver.

Answers

Answer:

Glucagon signals the liver to start producing glucose.

Explanation:

Glucagon signals the liver to start producing glucose from the breakdown of amino acids through gluconeogenesis process. When the glucose level decreases in the body due to fasting or starvation, the pancreas release glucagon which provides signals to the liver to produce glucose form non carbohydrates substances present in our body. This production of glucose increases the concentration of glucose in the blood and the body used this glucose for the generation of energy molecules in the form of ATP.

An unknown organism is found in the forest and the gene is sequenced as follows:

Unknown: C C A T G G A A T C G A

What kind of an animal do you think this is? Explain why:

Answers

Answer:

I beleive its somthing close to a  pig

Explanation:

The DNA sequence is closest to the pig . The amino acids Gly, Thr, Leu, and Ala are from the "unknown animal." On the worksheet, the pigs amino acids are Gly, Thr, Phe, and Ala. There is only one different amino acid between the two.

I might be wrong but there u go

8. Some humans have attached earlobes, some have detached
earlobes. Is this beneficial, neutral, or harmful? Why?

Answers

Answer:

attached earlobes are neutral

Explanation:

there are myths that attached earlobes are a sign of inbreeding but this has been unproven for years. they serve no difference

name the consumers:

scavengers, omnivores, herbivores, carnivores

Answers

Answer:

Jaguars and frog are carnivorous, lizard is omnivorous, whereas sloth, monkey and butterfly

Explanation:

Jaguars, frog, lizards, are carnivorous which feeds on meat of other organism whereas sloth, monkey and butterfly are herbivorous which feeds on plants. Jaguar feeds on Capybaras, deer, tortoises, fish, birds and monkeys etc, frog feeds on insects and lizard also eats insects as well as plants so it is omnivorous while on the other hand, sloth lives on the trees so feed on plants, butterfly feeds on flower's nectar and monkey feeds on the fruits present on the trees.

The
absorbs most of the nutrients obtained from our diet.

Answers

Absorption of nutrients mostly occurs in the small intestine.

Ans: Stomach acid helps to further digest or break down food and nutrients like protein. Not much gets absorbed directly in the stomach, except alcohol. Absorption of nutrients mostly occurs in the small intestine.

describe the pathway of water molecules from the soil through a plant and into the atmosphere ?​

Answers

sience plants absorb carbodieoxide the water molecules that is absorbed by the plant bond with carbondieoxide to form oxygen and release ìt into the atmosphere

Where are ribosomes produced?

Answers

Answer:

In bacterial cells, ribosomes are synthesized in the cytoplasm through the transcription of multiple ribosome gene operons. In eukaryotes, the process takes place both in the cell cytoplasm and in the nucleolus, which is a region within the cell nucleus.

Hope this helps!

state another hormone that influences the concentration of glucose in the blood apart from insulin and glucose​

Answers

Answer:

Amylin

Epinephrine

cortisol

growth hormone

Urgent question!!!!!

Is a thermometer dependent or an independent variable

Answers

Answer:

thermometer dependent variable

Differential between the life cycle of saprophytic fungus (mucor) and parasite fungus (pythium)

Answers

i am not in collage put i think the Answer is : that they have different organ

systems


Della and Nick drove at constant
speed from town A to town B along
the same road. The distance between the
two towns was 210 km. Nick left
Town A half an hour later than Della
Both of them arrived in Town B at
the same time . Della drove at a
Speed of 60 km/h Find Nick's speed.
Plzz help mee​

Answers

Answer:

70 km/hr

Explanation:

Distance between town A and B = 210 km

Della's speed = 60 km/hr

Time taken by Della = distance / speed

Time taken by Della = 210 km / 60km/ hr

Time taken by Della = 3.5 hours

Since Nick left half an hour later Than Della ;

Then, Nick's travel time = 3.5 - 0.5 = 3 hours

Nick's average speed = Distance /time

Nick's average speed = 210 / 3 = 70km/hr

The 'good' bacteria living in a
digestive tract to remove other
forms of bacteria is an example of...
A. a parasite.
B. mutualism.
C. commensalism.

Answers

Answer:

It's B

Explanation:

Mutualism: An interaction between individuals of different species that results in positive (beneficial) effects on per capita reproduction and/or survival of the interacting populations.

Digestive bacteria and humans. Inside our bodies is what we call good bacteria(mutualism), which aids in digesting the food we take.

why should the ureter be less robust than the vas deferens

Answers

Answer:

The vas deferens, also called ductus deferens, is part of the male reproductive system of many vertebrates; these ducts transport sperm from the epididymis to the ejaculatory ducts in anticipation of ejaculation. It is a partially coiled tube which exits the abdominal cavity through the inguinal canal.

Explanation:

In the glycolytic pathway, the enzyme triose phosphate isomerase catalyzes the production of glyceraldehyde 3-phosphate (GAP) from dihydroxyacetone phosphate (DHAP). GAP continues through glycolysis via the reaction catalyzed by glyceraldehyde 3-phosphate dehydrogenase. In a muscle (myocyte) cell culture model, anaerobic conditions lead to cell death when triose phosphate isomerase is inhibited. In this same model, cells with inhibited triose phosphate isomerase survive under aerobic conditions. Briefly explain these findings including a discussion of energy production in anaerobic versus aerobic metabolism.

Answers

Answer:

In an anaerobic environment, organisms convert glucose into ethanol or lactate. In this condition, glycolysis generates 2 molecules of ATP and one molecule of NADH with the pyruvate. NADH molecule supports glycolysis by oxidized to NAD+ during the fermentation process.

Here one 6-C molecule breaks into two molecules G3P and DHAP, TPI or triose phosphate isomerase interconvert these two isomers and each triose is responsible for the production of two ATP molecules but if TPI is inhibited then there will be one ATP production which is not enough for supporting cellular process under the absence of oxygen and cell do not survive.

But in aerobic conditions pyruvate converted to acetyl CoA which produces 3 NADH, 1 FADH2, and 1 ATP that will get enough ATP by the oxidative phosphorylation hence will survive even if TPI is inhibited.

I need help!

What significance does density have for earth?

Answers

Answer:

The atmosphere and Earth's interior are layered by density. Gravity pulls more strongly on denser materials so denser materials are at the center of things. Earth's core, at its center, is denser than its crust. The lowest layer of the atmosphere is denser than the upper layer.

Evolution acts at the level of the

Answers

Answer:

evolution acts on phenotypes on the individual level but evolution is at the population level

Explanation:

hope this helped follow me

mark brainliest^_^

It is not enough to be in the presence of a pathogen like a bacteria or virus, there also needs to be an environment in which the pathogen can grow.

a. True
b. False

Answers

This question seems a bit incomplete. It is not enough to be in the presence of a pathogen to...what? I assume the missing part is “to get sick” or something similar.

In any case, the answer would be true. If a pathogen is in an environment that is hostile or otherwise not conducive to its survival and growth, it usually wouldn’t be able to infect the host.

5) Taxonomy is defined as the science of
a. classifying plants according to their uses in agricultural experiments.
b. studying fossils
c. grouping organisms according to their characteristics and evolutionary history.
d. studying reproductive mechanisms in different species

Answers

Answer:

c - grouping organisms according to their characteristics

Explain what is “bottleneck” and why genetic drift is more powerful in small populations.

Answers

Answer:

because more is being forced through

Explanation:

Answer:

A bottleneck in genetics is an event that kills off or isolates a large portion of the population.

Explanation:

The more individuals in a population, the greater the genetic diversity. A genetic bottleneck happens when the number of individuals in a population gets greatly reduced. This happens as a result of random chance. For example, a storm might come through and knock down a bunch of trees. Birds that live in the trees may get harmed and those that survive now have to find a new home, so they migrate to other areas leaving behind a small population. This is important because the fewer individuals there are, the less genetic diversity and therefore, the less ability of the population to adapt. If you have lots of different genes available and a new disease arises in the population, it's more likely that some individuals will survive. If you only have a few genes available, it is less likely any individuals will survive. This can lead to really quick adaptation and evolution of a species.

What is the general relationship between distance from the sun and a planets density?

Answers

Answer:

There is no connection between a planet's distance from the Sun and its rate of rotation (spin on its own axis or Planetary Rotation Period). While it is true that, generally speaking, the gas giants have higher rotation rates than the earth like planets closer to the Sun, any correlation is only coincidental.

Explanation:

sum it up

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