A serial dilution is performed on a culture of Saccharomyces cerevisiae (Brewer's yeast). A 1:10 dilution was performed in series seven times. You plated and spread 100 microliters of the fifth dilution to a YEPD agar plate to grow overnight. After incubation, you count 142 colonies on the plate. What was the viable count of the stock culture

Answers

Answer 1

Answer: CFU/mL = 14200 CFU/mL

Explanation:

Microorganisms are very small and difficult to count directly. Instead, a laboratory technique is used to count CFUs (Colony Formation Units). This involves making dilutions of a microbial suspension and spreading it on thin layers of agar containing nutrients for growth in Petri dishes also called agar plates. After incubation under ideal conditions, some plates are completely covered with microbial growth; some have little or no growth; and some are covered with individual isolated "spots", which are microbial colonies. Since a colony may consist of a single organism or a group of microbes, the dots represent a CFU.

To calculate the viable count of the stock culture, you have to count the number of colonies, multiply by the dilution and the result will be the plate count in CFU.

So, the equation is:

CFU/mL = Number of colonies / (Volume trasferred to plate x dilution blank)

100 microliters = 0.1 mililiter

1/10 = 0.1 (dilution factor)

Replacing the information from the question:

CFU/mL = 142 / (0.1 mL x 0.1)

CFU/mL = 14200 CFU/mL


Related Questions

Plants contains many cells (multi-cellular) and are called

Answers

Answer organism

Explanation:

Answer:

Eukaryotic cells.

Explanation:

Plant and animal cells are eukaryotic, meaning that they have nuclei. Eukaryotic cells are found in plants, animals, fungi, and protists.

All multi-cellular organisms are Eukaryotic.

I hope this helps!

According to the Punnett Square shown above
A.
there is a 50% probability that an offspring will have brown eyes and a 50% probability that an offspring will have blue eyes.

B.
there is a 75% probability that an offspring will have blue eyes and a 25% probability that an offspring will have brown eyes.

C.
there is a 75% probability that an offspring will have brown eyes and a 25% probability that an offspring will have blue eyes.

D.
there is a 100% probability that an offspring will have one brown eye and one blue eye.

Answers

Answer:

C. There is a 75% probability that an offspring will have brown eyes and a 25% probability that an offspring will have blue eyes.

Explanation:

Note that the brown eyes is the dominant trait, denoted by B, while blue eyes is the recessive trait, denoted by b. In all cases of the Punnett square, if there is a dominant and recessive trait, the dominant trait will always come out on top.

The given Punnett square:

[tex]\left[\begin{array}{ccc}&B&b\\B&BB&Bb\\b&Bb&bb\end{array}\right][/tex]

Note that there are 3 options (BB, Bb, Bb) respectively, in which there is the dominant trait B (brown eyes). Only in one case is there only the recessive trait b (blue eyes), or a 1/4 chance of obtaining blue eyes. Therefore, there is a 3/4 chance of obtaining a brown eye:

C. There is a 75% probability that an offspring will have brown eyes and a 25% probability that an offspring will have blue eyes.

There is a 75% probability of brown eyes and a 25% probability for blue eyes

Which is the best description of thrust fault?
O A. A high-angle fault under tension
B. A low-angle fault under tension
C. A low-angle fault under compression
A
O D. A high-angle fault under compression

Answers

Answer:

D: A high-angle fault under compression

Explanation:

Answer:

A high-angle fault under compression

Explanation:

Plzplz ppz help with this riddle! Links will be reported. Thank you!

Answers

Answer: Hello Luvv

Green st patrick's day.

Explanation:

Hope this helps

Mark me brainset please.

Anna ♥

Answer: Hello!....

Patricks Day

Explanation:

Mark me brainest please. Hope I helped! Hope you make an 100%

-Anna♥

PLEASE HELP ASAP!!!


13. Populations of organisms grow at an _______ rate, but ____ factors such as food, habitat, predators, weather, disease, climate, & temperature can affect the maximum population size an area can support.

14. Sea otters are an example of a _________ species where the effects of overhunting this population of organisms will severely affect the population #s of other species.

15. _______ is determined by the # of species in a specific area as well as the # of different kinds of species in a specific area.

Answers

Answer:

. Exponential, limiting

14. Keystone

15.  ecosystem

Explanation:

Which of these is a long-term human health issue likely caused by air pollution?

A. Bioremediation
B. Damaged hearing
C. Dementia
D. Heart disease

Answers

Answer:

D

Explanation:

Heart disease is a long-term human health issue that is likely caused by air pollution. Thus, the correct option is D.

What is Air pollution?

Air pollution may be defined as any unwanted or contaminated impurities present in the air of living surrounding that causes harmful health effects to organisms.

Dementia is caused by the loss of nerve cells and their significant connections in the brain and is not likely the source of air pollution.

Majorly heart diseases are caused by the inhalation of contaminated air that forms a layer of harmful substances in the heart and lungs. Some of them include lung cancer, cardiovascular death, asthma, etc.

Therefore, the correct option for this question is D.

To learn more about Heart diseases, refer to the link:

https://brainly.com/question/13135199

#SPJ2

The number of different species living in a defined area is known as which of the following?

• bioconcentration
• habitat
• preserve
• biodiversity​

Answers

Answer:biodiversity

Answer:

Biodiversity

Explanation:

"Biodiversity is the biological variety and variability of life on Earth. Biodiversity is a measure of variation at the genetic, species, and ecosystem level. Terrestrial biodiversity is usually greater near the equator, which is the result of the warm climate and high primary productivity. "

- wiki

All of these are true about carbon fiber parts EXCEPT that: damage may not be visible from the front side of the part. repairs may not be allowed by some vehicle makers. they are a combination of fiberglass and carbon fibers. the carbon fiber cloth may be visible on some panels.

Answers

Answer:

they are a combination of fiberglass and carbon fibers.

Explanation:

Carbon fiber is made from organic polymers, which consist of long strings of molecules held together by carbon atoms. About 90% carbon fibers are made  up of polyacrylonitrile (PAN) process whereas very small amount about 10% are manufactured from rayon or the petroleum pitch process. carbon fibers are stronger than fiberglass and very expensive due to usage in many industries.

How does the washing of hands help prevent a societal problem

Answers

Answer:

It reduces the spread of disease.

Explanation:

np give me brainliest and thank me plz

So you want spread germs!!

Using examples where supportive, discuss the following statement, "From vaccinations and diagnostics, to explaining the root causes of many diseases, immunology is having a profound impact on life today"

Answers

Answer:

Yes.

Explanation:

Yes, immunology is having a profound impact on life today because this immunology provides information about the study of immune system. To find out the root cause of many disease, the study of immune system and its responses against many diseases are important in order to counter the infection. The immune system protects us from infection through various defense mechanism. If the immune system is not working properly, it can result in disease, such as autoimmunity, allergy and cancer etc.

What is an example of a problem that a chemical engineer solves.

Answers

Answer:

Well, it would be easier if I had choices, but here's what I found:

"Chemical engineers also develop solutions to environmental problems, such as pollution control and remediation. And yes, they process chemicals, which are used to make or improve just about everything you see around you.""Another source mentioned that they use chemistry to solve everyday problems. They also help make [them] as environmentally safe as possible, and eliminate as much waste as they can."

Hope this helps! :)

What types of molecules make up the cell membrane?

Answers

Answer:

The double layers of cell membranes are made up of PHOSPHOLIPIDS.

The cell membrane, also called plasma membrane is made up of double layers of phospholipids.  The Phospholipid is a type of lipid, which is made up of two parts, an hydrophillic head, and a hydrophobic tail. The hydrophillic head is made up of phosphate and glyerol while the hydrophobic tail is made up of fatty acids. The double layer of phospholipids serves as a barrier that allows only certain substances to enter into the cells.

Explanatiion:

I searched it up : p

An organisms phenotype is

A. Its parents genes

B. Its genetic make up

C. Its physical appearance

D. Its heterozygosity​

Answers

Answer:

C) Its physical appearance

Explanation:

The way I remember what phenotype is, is that both physical and phenotype begin with a p.

Hope this helped :)

C. Pheno= physical Geno= genes

factors that affect one's growth​

Answers

genetics, nutrition, fitness, sleep etc.

Answer:

10 Factors That Influence the Growth and Development of a Child:

HeredityEnvironmentSexExercise and HealthHormonesNutritionFamilial InfluenceGeographical InfluencesSocio-Economic Status Learning and Reinforcement

Explanation:

You are studying a disorder that is based on the genetic composition at three loci. Assume that a dominant allele at any locus adds 5 units of risk for the disorder and that a recessive allele at any locus adds 3 units of risk for the disorder. Individuals with 26 or more units of risk develop the disorder. The environment does not affect the presence or absence of this disorder. How many risk units will be present in an individual of genotype AABbcc

Answers

Answer:

24

Explanation:

Given that:

At either locus, the expression of the dominant allele increases the risk by 5 units.

The assigned genetic sequence "AABbcc" has three dominant alleles, implying that it increases risk by 15 units.

At any locus, the expression of the recessive allele increases the risk by three units. The assigned genetic sequence "AABbcc" has three recessive alleles, which means it increases the chance by 9 units.

In an individual with the genotype AABbcc, the total number of risk units is = 15+9 = 24. This implies that the person does not develop the disease).

During the normal cardiac cycle, the bicuspid valve opens when: the pressure in the right ventricle is greater than in the right atrium b. the pressure in the right atrium is greater than in the right ventricle c. the pressure in the left atrium is greater than in the left ventricle d. the pressure in the left ventricle is greater than in the left atrium e. the pressure in the right ventricle is greater than in the pulmonary artery

Answers

Answer:

b. the pressure in the right atrium is greater than in the right ventricle

Explanation:

A bicuspid valve is an aortic valve that only has two leaflets, instead of three. The function of aortic valve is to regulates blood flow from the heart into the aorta. The valve opens and closes because of pressure differences. The opening of the valve occurs when the pressure is greater in the left atrium than ventricle whereas, closing happens when there is greater pressure in the left ventricle than atrium so we can conclude that the pressure in the right atrium is greater than in the right ventricle.

Phalloidin is a toxin from a toadstool (mushroom). You are studying actin dynamics and the effect of phalloidin. You add G-actin and ATP to a test tube and allow the reaction to come to equilibrium. This produces the light scattering plot below. When you add phalloidin, what will happen to the line?

Answers

Answer:

Dissociation of actin subunits occurs.

Explanation:

When we add phalloidin to a solution containing G-actin, the phalloidin binds to actin filaments more tightly as compared to actin monomers which leads to a decrease in the constant rate of dissociation of actin subunits from the ends of the filament. This dissociation stabilizes the actin filaments through the prevention of filament depolymerization. So we can conclude that addition of phalloidin to actin leads to stabilizing of actin filaments.

How do adaptations help species manage competition

Answers

Answer:

Adaptations create a unique niche that is not shared by organisms of another species, thus managing competition for resources between species.

why do the body need specialized cells?​

Answers

Answer: Hello,  There! your Answer  is Below!!

specialized cells allow for different types of tissues to exist in our organs, so that the organs can perform different functions in our organ systems.

Explanation:

Cell specialization allows new cells to develop into a range of different tissues, all of which work together to make living organisms function as a whole.

Hope this helps!!!

Have a great day!!!

Describe how and why this happens (try to do an easy description)
A comet in space continues to move without slowing down or slowing up.

Answers

Answer:

because there is no force acting in the comet making it able to go on forever without change in its path  or speed

explanation

newton's first law

Be sure to answer all parts.
Find the pH and the volume (mL) of 0.407 M HNO3 needed to reach the equivalence point in the titration of 2.65 L of 0.0750 M pyridine (C5H5N, Kb = 1.7 × 10−9).
Volume = mL HNO3
pH =

Answers

Answer:

pH = 3.215

Explanation:

From the given information;

Using the equation for the dilution of  a stock solution:

Since moles of C5H5N = moles of HNO3

Then:

[tex]M_{C_5H_5N}\times V_{C_5H_5N}= M_{HNO_3}\times V_{HNO_3}[/tex]

[tex]0.0750 M \times 2.65 L = 0.407 M \times V_{HNO_3}[/tex]

[tex]V_{HNO_3}= \dfrac{0.0750 M \times 2.65 L}{0.407 M}[/tex]

[tex]V_{HNO_3}=488.33 \ mL[/tex]

The reaction between C5H5NH and H2O is as follows:

[tex]C_5H_5N^+H + H_2O[/tex] ⇄ [tex]C_5H_5N + H_3O^+[/tex]

[tex]Molarity \ of \ C_5H_5N^+H = \dfrac{moles}{addition \ of\ the\ total\ volume}[/tex]

[tex]Molarity \ of \ C_5H_5N^+H = \dfrac{0.0750 \ M \times 2.65 \ L}{2.65 \ L + 0.48833 \ L}[/tex]

[tex]Molarity \ of \ C_5H_5N^+H = \dfrac{0.19875 \ ML}{3.13833 L }[/tex]

[tex]= \ 0.06333\ M[/tex]

Now, the next step is to draw out the I.C.E table.

    [tex]C_5H_5N^+H + H_2O[/tex] ⇄ [tex]C_5H_5N + H_3O^+[/tex]

I       0.06333                      0          0

C        - x                               x          x  

E      0.06333 -x                   x          x

[tex]K_a = \dfrac{10^{-14}}{1.7 \times 10^{-19}} \\ \\ K_a = 5.8824 \times 10^{-6}[/tex]

[tex]K_a = \dfrac{[C_5H_5N][H_3O^+] }{[C_5H_5N^+H] } \\ \\ 5.8824 \times 10^{-6} = \dfrac{x^2}{0.06333 - x}[/tex]

Assuming x < 0.06333

[tex]x^2 = 5.8824 \times 10^{-6} \times 0.06333[/tex]

Then

[tex]x^2 = 3.72532392 \times 10^{-7} \\ \\ x= \sqrt{3.72532392 \times 10^{-7}} \\ \\ x = 6.1035 \times 10^{-4} \ M[/tex]

[tex][H_3O^+] = x = 6.1035 \times 10^{-4} \ M \\ \\ pH = -log (6.1035 \times 10^{-4}) \\ \\ \mathbf{\\ pH = 3.215}[/tex]

The pH for  HNO3 needed to reach the equivalence point in the titration of 2.65 L of 0.0750 M pyridine (C5H5N, Kb = 1.7 × 10−9) is

HNO3 + C5H5N→CSH5NH + + NO3

Moles of C5H5N = volume x concentration

=2.65 x 0.0750

=0.1987 mol

Moles of HNO3 needed = moles of C5H5N = 0.19875 mol Volume of HNO3 eeded = moles / con

=0.19875/0.54

=0.363L

=363 mL

At the equivalence point, most effective C5H5NH + a NO3-are Moles of C5H5NH+ = moles of C5H5N = 0.19875 mol

Total extent of answer n = 2.65 + 0.363 =3.013 L

Initial concentration [ C5H5NH + ] o = moles/ volume =0.19875/3.013 =0.06596 M

Kb of C5H5N = 1.7 x 10 ^  9

By fixing the equation,

H + = 6.199 * 10 ^ 4 M

pH=-log[H+]

=3.207

=3.21 pH

What is pH ?

The pH is the degree of ways acidic or simple a substance is. In humans, pH stability performs a position in preserving the frame functioning optimally. The best pH of the frame is barely alkaline, which helps sure biochemical reactions like oxygenating the blood.

Thus it is clear above.

To learn more aboyt the titration refer to link :

https://brainly.com/question/186765

NASA's Solar Dynamics Observatory is
a. landing rovers on the suns surface
b. providing high clarity images of the sun
c. two of these
d. examining the suns innermost core

Answers

Answer:

b. providing high clarity images of the sun.

Explanation:

NASA is an acronym for National Aeronautics and Space Administration which is an agency of the federal government of the United States of America. It is saddled with the responsibility of exploring and making researches about space.

On the 5th of September, 1977, the National Aeronautics and Space Administration (NASA) launched a spacecraft known as "Voyager 1" to study and explore the outer planets of the solar system.

Additionally, two voyager spacecraft were built for the above mentioned study and exploration exercise; Voyager 1 and Voyager 2. Voyager 2 was actually the first spacecraft while Voyager 1 was launched sixteen (16) days after and was faster.

Similarly, the Solar Dynamics Observatory (SDO) was launched on the 11th of February, 2010.

Typically, it's a geosynchronous-orbiting satellite designed to provide scientists with a great volume of informations about the influence of the sun in the earth.

NASA's Solar Dynamics Observatory is providing high clarity images of the sun.

During prophase in both mitosis and meiosis, chromosomes condense. At this time, identical copies of chromosomes are connected. What is each copy of a chromosome called? ______________________

Answers

Answer:

Well, DNA's arranged in chromosomes, as you know, so what happens is, as a chromosome replicates, or makes a copy of itself, it's arranged as two chromosomes next to each other, called chromatids.

Explanation:

Well, DNA's arranged in chromosomes, as you know, so what happens is, as a chromosome replicates, or makes a copy of itself, it's arranged as two chromosomes next to each other, called chromatids.

Answer: sister chromatids

Explanation: since each of the parent's cells chromosomes were replicated during interphase, there are two copies of each chromosome in the cell during prophase

What is the greatest source of carbon that enters the ocean
from the atmosphere?
a
carbon monoxide
w
b carbon dioxide
C
bicarbonate ions

Answers

Answer:

c.)carbon dioxide

Explanation:

Most carbon enters the ocean when carbon dioxide in the atmosphere dissolves in ocean water. When carbon dioxide dissolves in water (H2O), it forms an acid called carbonic acid (H2CO3). The reaction is given by the equation: CO2 + H2O ↔ H2CO3.

As the amount of light received by this plant increases, its rate of
photosynthesis HELP!!!!!!

Answers

D. Increases, then remains the same

As seen on the graph, the amount of photosynthesis rises and then maintains in a straight line, meaning it is staying the same. Hope that helps!!

At high temperatures enzyme activity declines
because of:

Answers

Answer:

protein get denatured

Explanation:

at high temperatures enzyme activities in our body decline due To the proteins getting denatured

Return to the MyPlate food plan calculator, and make an eating plan for someone whose weight and age are different from yours. You can change the person’s age, gender, and activity level. How does this checklist differ from yours? Why do you think the person’s energy needs are different?

Answers

Answer:

Increase consumption of food to increase weight.

Explanation:

There is my friend who is very weak and thin about 52 kg weight and he is one year older than me. I wanted to increase his weight up to 70 kg so I will recommend him to eat more nutritive food having large amount of proteins because proteins made muscles in the body. I suggest him to eat more food and exercise regularly to have a muscular body. The checklist is different from my checklist due to different goals. I wanted to lower my weight and increases the weight of my friend. My friend's energy needs are different from mine due to different goals to achieve.

Answer:

Males generally need more calories than females. Because men have an overall larger body in both height and weight, they need to consume more calories. For a male who is 20 years old, 5 feet and 10 inches, weighs 198 pounds, and has moderate physical activity, he should consume about 2,800 calories per day. That is about 1,000 calories more than my average caloric intake. To consume that many calories, he should eat 2 ½ cups of fruit, 3 ½ cups of vegetables, 7 ounces of protein, 3 cups of dairy, and 10 ounces of grains per day.

Explanation:

PLEASE HELP ME ON BOTH QUESTIONS ASAP!!!!! GIVING 13 POINTS AWAY!!!!!!

Answers

Answer:

the awnser for the first question i think is D because a funnel is used to clean water of contaminants.

Explanation:

the awnser for the second question is A.

Which of the following has the highest sodium content?

A) Fresh fruits and vegetables
B) Lunchmeats, processed cheeses and canned soup
C) Mild, roast beef sandwich
D) Tossed salad with vinegar and oil dressing​

Answers

Answer:

b

Explanation:

I think that's right since canned food has a lot of sodium to help preserve

For hair color, having dark/brunette hair is dominant to having light or blond hair. Olivia Keller is blond but her mother, Mrs. Keller is brunette. Mr. Keller is also brunette. Use a Punnett Square to show whether Olivia could be the Kellers’ daughter.

Answers

Answer:

Olivia could be the Keller's daughter if both parents are heterozygous for their brunette hair coloration. Please find the punnet square in the attachment section.

Explanation:

According to this question that involves a gene coding for hair color in humans, the allele for dark/brunette hair (B) is dominant to the allele for light/blond hair (b). This means that a heterozygous individual (Bb) will have a brunette hair coloration.

Olivia Keller is said to have blond hair but her mother, Mrs. Keller and father, Mr. Keller both have brunette hair coloration. Since Olivia is blond (recessive), she must have a genotype "bb". However, this is possible if both of her parents i.e. Mr and Mrs. Keller have a heterozygous genotype (Bb).

From the punnet square of the cross, Bb × Bb, in the attachment section, it can be observed that brunette color (BB, Bb, Bb) and blond color (bb) were produced in the offsprings in the ratio 3:1. This means that it is very likely that Olivia could be the Keller's daughter.

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