Burst size of bacterial virus is the number of viral particles released from an infected cell following the lysis of the host cell. The burst size is the number of progeny virions that is liberated per infected bacterial cell. Bacteriophages are viruses that infect bacteria, they usually have a rapid rate of replication and lytic infections.
In the study of bacteriophages, the burst size is a crucial factor that is measured. It is essential for determining the rate of viral replication and lytic infection that will occur under specific conditions. The following steps would be taken to calculate the burst size for a bacterial virus under the following conditions:Given: The growth medium was inoculated with 300 phage infected E. coli/ml and at the end of the experiment, 6x104 virus particles/ml were obtained.
This implies that Burst size = (6x104 virus particles/ml)/(300 phage infected E. coli/ml) = 200 virus particles/infected cell. The Burst size of the bacterial virus under the specified conditions is 200 virus particles/infected cell.2. The purpose of a plaque assay for bacteriophage:A plaque assay is a standard technique that is used to determine the concentration of phage particles that are present in a liquid. It is an essential tool for measuring the infectivity of a bacteriophage population. The purpose of a plaque assay for bacteriophage is to quantify the number of viral particles that are in a given sample. The number of viral particles in a given sample is determined by counting the number of plaque-forming units (PFUs).3.
Why must the multiplicity of infection (MOI) be low for plaque assay?In a plaque assay, a low multiplicity of infection (MOI) is required to ensure that each bacteriophage will infect only one bacterium. A low MOI means that the number of phages is much less than the number of bacteria. When MOI is too high, two or more phages can infect the same bacterium, resulting in a more complicated set of plaques to count. Therefore, it is recommended that the MOI be kept at a minimum to ensure the accuracy of the assay.
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Metabolic fates of newly synthesized cholesterol are all but one. Choose the one. Olipoproteins bile salts O NAD+ membrane Question 12 (1 point) of the following types of lipoprotein particles, choose
The metabolic fates of newly synthesized cholesterol include lipoproteins, bile salts, and membrane incorporation. NAD+ is not a metabolic fate of newly synthesized cholesterol. Option a is correct.
After synthesis, cholesterol undergoes various metabolic pathways in the body. One major fate of cholesterol is its association with lipoproteins. Lipoproteins are complexes of lipids and proteins that transport cholesterol and other lipids through the bloodstream. These lipoproteins include low-density lipoprotein (LDL) and high-density lipoprotein (HDL). LDL carries cholesterol from the liver to the peripheral tissues, while HDL helps transport excess cholesterol from peripheral tissues back to the liver for excretion.
Another fate of cholesterol is its conversion into bile salts. Bile salts are synthesized in the liver from cholesterol and are essential for the digestion and absorption of dietary fats. Bile salts are stored in the gallbladder and released into the small intestine during the digestion process.
Cholesterol can also be incorporated into cell membranes. It is an important component of cell membranes and plays a crucial role in maintaining their integrity and fluidity.
However, NAD+ is not a metabolic fate of newly synthesized cholesterol. NAD+ (nicotinamide adenine dinucleotide) is a coenzyme involved in various metabolic reactions, particularly in redox reactions. It is not directly involved in the metabolism or fate of cholesterol.
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The Complete question is
Metabolic fates of newly synthesized cholesterol are all but one. Choose the one.
a. lipoproteins bile salts
b. NAD+ membrane Question 12 (1 point) of the following types of lipoprotein particles, choose the one
a. lipids through the bloodstream
b. maintaining their integrity and fluidity
What is the cause of the evolution of senescence according to the two evolutionary hypotheses (Mutation Accumulation and Antagonistic Pleiotropy)? a)mutations accumulate in individuals as they grow older, leading to senescence
b)mutations that have pleiotropic fitness effects are removed by selection
c)mutations that cause deleterious fitness effects late in life are effectively neutral
d)mutations that cause deleterious fitness effects late in life experience a strong "force of natural selection"
The Antagonistic Pleiotropy theory postulates that senescence results from the presence of mutations that have advantageous early-life effects but unfavourable late-life consequences.
The two evolutionary explanations for senescence provide the following descriptions of the causes: According to the hypothesis of mutation accumulation, as people age, they accumulate harmful mutations that lead to senescence. These mutations can remain and cause a reduction in fitness and ageing because natural selection is less successful at getting rid of mutations that have late-life effects. The Antagonistic Pleiotropy theory postulates that senescence results from the presence of mutations that have advantageous early-life effects but unfavourable late-life consequences. Because the advantages of these mutations early in life outweigh the disadvantages of senescence later, they are kept in the population. Therefore, the appropriate response is: a) as people age, mutations increase, leading to senility
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Examine this pedigree for a rare human disease and determine the most likely mode of inheritance. If person II-3 and person III-1 had a child, what would be the probability of that child having the disease? a) zero chance b) 1/4 c) 100 percent d) 1/2
Based on the pedigree, the most likely mode of inheritance for the rare human disease is autosomal recessive.
In an autosomal recessive inheritance pattern, individuals need to inherit two copies of the disease-causing allele (one from each parent) in order to express the disease. In this pedigree, individuals II-3 and II-4 are unaffected but both carry one copy of the disease-causing allele, making them carriers. Their child, III-1, expresses the disease, indicating that both II-3 and II-4 must have passed on their disease-causing alleles to III-1.If person II-3 and person III-1 were to have a child, the probability of that child having the disease would be 1/4 or 25 percent. This is because person II-3 is a carrier (heterozygous) and person III-1 is affected (homozygous recessive). When they have a child, there is a 25 percent chance that the child will inherit two copies of the disease-causing allele and therefore express the disease. The other possible outcomes include a 50 percent chance of the child being a carrier like II-3 or a 25 percent chance of the child being unaffected.
It's important to note that this probability assumes that both II-3 and III-1 are correctly identified as carriers and affected, respectively, based on their phenotypes and genetic testing.
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An ORF is a continuous stretch of codons that begins with a start codon (usually AUG) and ends at a stop codon (usually UAA, UAG or UGA). The gene x has 920 codons. In a particular tissue, the base ‘C’ of 440th codon CAA (codes for glutamine) of gene x is edited to the base ‘U’. Answer the following question? Enter only a number in the provided space.
How many amino acids will be in the protein X from the un-edited mRNA Answer
How many amino acids will be in the protein Xedit from the edited mRNA? Answer
How many nucleotides will be in the open reading frame of gene Xedit from the edited mRNA?
An ORF is defined as a continuous sequence of codons that starts with a start codon and terminates at a stop codon. Gene X has 920 codons, and in a specific tissue, the C base of the 440th codon (CAA) of Gene X was replaced with a U base.
The number of amino acids in a protein is directly linked to the number of codons in the mRNA; since Gene X has 920 codons, Protein X will have 920/3 = 306 amino acids (since each codon codes for one amino acid, and there are three nucleotides in each codon).Therefore, the number of amino acids present in protein X from the unedited mRNA is 306 amino acids.
When the 440th codon (CAA) is edited by replacing the C base with a U base, the resulting codon becomes CUA, which codes for leucine rather than glutamine. The edited mRNA encodes a different protein, and the number of amino acids present in this protein is determined by the number of codons in the edited mRNA.
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Explain the common cold and flu of viral origin.
Explain the disease caused by the varicella-zoster virus
Explain the disease measles, mumps and rubella
Common cold and flu are respiratory illnesses caused by viral infections, with the common cold usually caused by rhinoviruses and the flu by influenza viruses. Varicella-zoster virus causes chickenpox (varicella) and later reactivates as shingles (herpes zoster).
Measles, mumps, and rubella are viral infections with distinct symptoms, with measles causing fever and a characteristic rash, mumps affecting the salivary glands, and rubella causing a rash and posing risks to pregnant women.
Common Cold and Flu:
The common cold and flu are both respiratory illnesses caused by viral infections. The common cold is usually caused by rhinoviruses, while the flu is caused by influenza viruses. These viruses are highly contagious and primarily spread through respiratory droplets when an infected person coughs, sneezes, or talks.
Varicella-Zoster Virus (VZV) Disease:
The varicella-zoster virus causes two distinct diseases. The primary infection results in chickenpox, also known as varicella. Chickenpox is highly contagious and spreads through direct contact or respiratory droplets. It is characterized by a blister-like rash, itching, fever, and general malaise.
Measles, Mumps, and Rubella:
Measles, mumps, and rubella are all viral infections that can cause distinct diseases. Measles, caused by the measles virus, is highly contagious and spreads through respiratory droplets.
Mumps, caused by the mumps virus, is also highly contagious and spreads through respiratory droplets or direct contact with infected saliva. It affects the salivary glands, leading to swelling and pain in the cheeks and jaw.
Rubella, caused by the rubella virus, is generally a mild infection but can have severe consequences if contracted by pregnant women. It spreads through respiratory droplets and causes a rash, low-grade fever, and swollen lymph nodes.
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which of the two if any does Digestion of food not occur? explain stomach or mouth?
The digestion of food occurs in the stomach and mouth. Digestion is the process of breaking down large molecules of food into smaller molecules that can be absorbed and used by the body.
Digestion begins in the mouth, where the food is physically broken down by chewing and mixed with saliva, which contains enzymes that begin the chemical breakdown of carbohydrates.The stomach is the next stop in the digestive process. It is a muscular sac that mixes the food with stomach acid and enzymes to further break down the food into a liquid called chyme. The stomach also releases the hormone gastrin, which triggers the release of more digestive juices in the small intestine, where the majority of digestion and absorption take place.In conclusion, both the mouth and stomach are involved in the digestion of food. The mouth is where the process begins, with the mechanical and chemical breakdown of food, while the stomach continues the process by mixing the food with digestive juices to break it down further.
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Chain Reactions Linking Acorns to Gypsy Moth Outbreaks and Lyme Disease Risk Oak trees (Quercus spp.) produce large autumnal acorn crops every two to five years, and produce few or no acorns during intervening years. Acorns are a critical food for white-footed mice (Peromyscus leucopus). Mice are important predators of the pupal stage of the gypsy moth (Lymantria dispar). This introduced insect periodically undergoes outbreaks that defoliate millions of hectares of oak forests, decreasing tree growth, survival, and acorn crop production. An abundance of acoms provides food for white-tailed deer (Odocoileus virginianus). Mice and deer are the primary hosts of the black-legged tick (Ixodes scapularis), which carries Lyme disease.
The chain of events linking acorns to gypsy moth outbreaks and Lyme disease risk can be summarized as follows:
1. Acorn Production: Oak trees produce large acorn crops every two to five years, with intervening years having few or no acorns.
2. White-Footed Mice: Acorns are a critical food source for white-footed mice. During years with abundant acorn production, the mouse population increases due to the availability of food.
3. Gypsy Moth Predation: White-footed mice play a crucial role in controlling gypsy moth populations. They are important predators of the pupal stage of the gypsy moth.
4. Gypsy Moth Outbreaks: The gypsy moth, an introduced insect, periodically undergoes outbreaks. During these outbreaks, the gypsy moth larvae defoliate millions of hectares of oak forests. This defoliation can have detrimental effects on tree growth, survival, and acorn crop production.
5. Deer and Acorns: An abundance of acorns provides a food source for white-tailed deer. Deer play a role in dispersing acorns and can consume large quantities when they are available.
6. Tick Hosts: Both white-footed mice and white-tailed deer are primary hosts for the black-legged tick, also known as the deer tick (Ixodes scapularis). These ticks are responsible for transmitting Lyme disease.
7. Lyme Disease Risk: The black-legged tick carries the bacterium that causes Lyme disease. When ticks feed on infected hosts, such as white-footed mice and white-tailed deer, they can acquire the bacterium. Subsequently, these ticks can transmit the bacterium to humans when they bite.
The chain reaction begins with the production of acorns by oak trees, which supports an increase in the population of white-footed mice. These mice, in turn, prey on gypsy moth pupae, helping to control gypsy moth populations. However, when gypsy moth outbreaks occur, they can defoliate oak forests and impact tree health and acorn production. Abundant acorns also attract white-tailed deer, which are hosts for black-legged ticks carrying Lyme disease. Thus, the interconnectedness of these factors contributes to the risk of Lyme disease transmission to humans.
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structures-structures with similar structure but differ in their function. The similar structure is due to having a common ancestor with that structure that was passed down. structures - structures with different structures/origin but the same function. These structures have a superficial resemblance due to convergent evolution, such as a bird and bat wing Convergent evolution is when two different species evolve for the same conditions (flying, swimming, etc.) parative Embryology The embryos of most animals develop in very similar S This is especially true for early stages of development cular Evidence (DNA & Proteins) Organisms share huge amounts of The is universal (used by all living things) DNA and proteins have been used to determine evolutionary Humans and chimpanzees share over. of their DNA W Most of the differences are in non-protein coding regions of the DNA
Structures with similar structure but differ in their function are known as homologous structures. The similar structure is due to having a common ancestor with that structure that was passed down. Homologous structures are used to find evolutionary relationships among organisms.
The study of comparative anatomy shows that the same basic structures of the body have been modified over time to serve various purposes. For example, the forelimbs of vertebrates are made up of the same bones, although they are used for different functions in different animals. This is because they have a common ancestor from which they evolved.Structures with different structures/origin but the same function are called analogous structures.
These structures have a superficial resemblance due to convergent evolution, such as a bird and bat wing. Convergent evolution is when two different species evolve for the same conditions (flying, swimming, etc.).Vestigial structures are structures that have no function but are remnants of structures that had a function in the ancestors of the organism. These structures may not have any function in the organism, but they may have had an important function in the organism's ancestors. DNA is a universal molecule that is used by all living organisms.
The genetic code is universal, and all organisms use the same code to build proteins. DNA and proteins have been used to determine evolutionary relationships among organisms. Organisms share huge amounts of DNA and proteins, and this similarity is used to determine their evolutionary relationships. Humans and chimpanzees share over 98% of their DNA. Most of the differences are in non-protein coding regions of the DNA.
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WHAT ARE THE LONG TERM EFFECT OF batrachotoxin TO THE
CIRCULATORY SYSTEM?
Batrachotoxin is a poisonous substance that is found in the skin of certain frogs and in some species of birds. This toxin acts on the sodium channels of the body. Batrachotoxin can have long-term effects on the circulatory system.
Batrachotoxin can lead to death, as it can cause respiratory and circulatory failure. Batrachotoxin causes sodium channels to remain open, allowing excessive amounts of sodium ions to enter the cells. As a result, the nerves and muscles of the heart are unable to function properly, leading to irregular heartbeat. Batrachotoxin can also lead to the accumulation of fluid in the lungs, making breathing difficult. The toxin can also cause swelling of the brain and seizures, leading to loss of consciousness.
the long-term effects of batrachotoxin to the circulatory system can be severe. Batrachotoxin is a poisonous substance that is found in the skin of certain frogs and in some species of birds. This toxin acts on the sodium channels of the body, causing nerves and muscles to be unable to function properly, leading to irregular heartbeat. Batrachotoxin can also cause the accumulation of fluid in the lungs, making breathing difficult, and swelling of the brain and seizures, leading to loss of consciousness. People who survive batrachotoxin poisoning may experience long-term effects, including heart disease, lung disease, and neurological problems. Therefore, the long-term effects of batrachotoxin on the circulatory system can be fatal and cause permanent damage.
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What are the two types of Speciation? 4.3
There are two pathways to speciation: PG: 137
1) Transformation: One species evolves into another species
2) Divergence: One or more species arise from a parent species
The two types of speciation are allopatric speciation and sympatric speciation.
Allopatric speciation: Allopatric speciation occurs when a population is geographically isolated, leading to reproductive isolation and the formation of new species. The physical barrier prevents gene flow between the separated populations, allowing for independent evolutionary changes to accumulate over time. The accumulation of genetic and phenotypic differences can eventually result in reproductive isolation, where individuals from the separated populations can no longer produce viable offspring if brought back into contact.
Sympatric speciation: Sympatric speciation occurs without geographic isolation, where a new species arises within the same geographic area as the parent population. Reproductive isolation is achieved through other mechanisms such as ecological, behavioral, or genetic factors. These mechanisms can lead to the development of reproductive barriers that prevent gene flow between different subgroups within the population. Over time, these subgroups accumulate genetic and phenotypic differences, eventually leading to the formation of new species.
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Targeting an Antibiotic Resistance Gene using CRISPR-Cas9
The rise and spread of antibiotic resistance in bacteria are alarming because of the impact on the cost, complications, and outcomes of treatment. Of particular concern are resistant bacteria that cause hospital-acquired infections (HAIs). Enterococcus faecalis, a member of the intestinal normal microbiota, is now a leading cause these infections. This organism is an opportunist, meaning that if the normal microbiota population is disturbed (for example by antibiotic treatment), it proliferates and becomes pathogenic. The pathogenic strains usually exhibit larger than normal genomes, having acquired mobile genetic elements such as plasmids, transposons, or phages (viruses that infect bacteria). Some of these elements contain antibiotic resistance genes.
Now a collaborative research team from the University of Texas at Dallas and the University of Colorado is investigating the use of CRISPR-Cas9 for overcoming antibiotic resistance in E. faecalis. Recall that bacteria use the CRISPR-Cas system as a defense mechanism, protecting them against the foreign DNA of mobile gene elements. CRISPR-Cas9 consists of an endonuclease (Cas9) that uses a guide RNA (gRNA) to locate and cleave foreign double stranded DNA at a specific site. For example, if a phage injects its DNA into a bacterial cell, that cell uses its CRISPR-Cas9 system to identify and destroy that phage DNA. The system also creates "memory" so that the bacterial cell is protected against future encounters with that same type of phage. Scientists can manipulate the CRISPR-Cas9 system by inserting specific gRNAs to target the Cas9 endonuclease to exactly where they want it to go in a genome, a potential tool for gene silencing or editing.
The research team previously showed that drug resistant E. faecalis does not have an intact CRISPR-Cas system; it lacks the Cas9 component and is thus susceptible to the uptake of foreign DNA. Now the team has developed a novel way of getting a functional CRISPR-Cas9 into those organisms in an effort to rid them of their antibiotic resistance genes. They engineered a plasmid, inserting genes for CRISPR-Cas9 along with gRNA sequences that are homologous to a resistance gene for the antibiotic erythromycin. The engineered plasmid was then introduced into a donor strain of E. faecalis that has conjugation ability. The presence of the CRISPR-Cas9 in the donor strain makes it immune to acquiring foreign DNA. When the donor strain conjugated with the drug resistant E. faecalis strain, the resistant strain gained a copy of the engineered plasmid containing the modified CRISPR-Cas9 system. The CRISPR-Cas9 in that cell then targeted its erthromycin resistance gene.
The team was able to show that the introduced plasmid significantly reduced the resistance of the resistant E. faecalis to erythromycin, making it sensitive to this drug. The work indicates that it may be possible in the future to use conjugation delivery of CRISPR-Cas9 antimicrobials.
Rodrigues, M. et. al. 2019. Conjugative Delivery of CRISPR-Cas9 for the Selective Depletion of Antibiotic-Resistant Enterococci. Antimicrob Agents Chemother. 63(11). pii: e01454-19.
Why is the genome of pathogenic Entercoccus aerogenes slightly larger than that of their nonpathogenic counterparts?
a. Pathogenic strains of Enterococcus have a CRISPR-Cas9 cassette and this makes them larger.
b. Pathogenic Entercoccus strains make the enzyme Dicer, so have an additional gene for this enzyme.
c. Pathogenic strains have acquired extra DNA in the form of a mobile genetic element (MGE).
d. Pathogenic strains do not have a larger genome; they have a double copy of their single chromosome.
e. Pathogenic strains of any bacterium are larger than nonpathogenic strains, and have larger genomes.
Pathogenic strains have acquired extra DNA in the form of a mobile genetic element (MGE).
The correct option to the given question is option c.
The genome of pathogenic Enterococcus aerogenes is slightly larger than that of their nonpathogenic counterparts because pathogenic strains have acquired extra DNA in the form of a mobile genetic element (MGE).
In particular, some of these elements contain antibiotic resistance genes. The pathogenic strains usually exhibit larger than normal genomes, having acquired mobile genetic elements such as plasmids, transposons, or phages (viruses that infect bacteria).
The acquisition of extra DNA in pathogenic strains makes it possible for them to proliferate and become pathogenic, especially if the normal microbiota population is disturbed, for example, by antibiotic treatment.Therefore,Pathogenic strains have acquired extra DNA in the form of a mobile genetic element (MGE).
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Transaminases need cofactor. Vitamin B6 Vitamin B3 Vitamin B9 Vitamin B12
The transaminases primarily rely on vitamin B6 as a cofactor, they do not require other B vitamins such as niacin (vitamin B3), folic acid (vitamin B9), or cobalamin (vitamin B12) for their enzymatic activity.
Transaminases are a group of enzymes that play a vital role in various biochemical reactions in the body, particularly in amino acid metabolism. These enzymes facilitate the transfer of amino groups between different amino acids, thereby allowing the synthesis of new amino acids and the breakdown of others.
To carry out their function, transaminases require a coenzyme known as pyridoxal phosphate (PLP), which is derived from vitamin B6.
Vitamin B6, also known as pyridoxine, is a water-soluble vitamin that serves as a cofactor for many enzymes, including transaminases.
It is involved in numerous metabolic reactions, including the conversion of amino acids and the synthesis of neurotransmitters and hemoglobin. Vitamin B6 is converted into its active form, PLP, which binds to transaminases and acts as a coenzyme, facilitating the transfer of amino groups.
These vitamins play essential roles in other aspects of metabolism but are not directly involved in transamination reactions.
Niacin (vitamin B3) is involved in energy metabolism and DNA repair, while folic acid (vitamin B9) is necessary for DNA synthesis and cell division.
Cobalamin (vitamin B12) participates in DNA synthesis, red blood cell formation, and nerve function.
Although these B vitamins are crucial for overall health and well-being, they do not serve as cofactors for transaminases.
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Which stores more potential energy: one molecule of glucose or two
molecules of pyruvate? explain.
One molecule of glucose stores more potential energy than two molecules of pyruvate. Glucose is a six-carbon molecule that is broken down through a series of chemical reactions into two molecules of pyruvate. This process is called glycolysis, which takes place in the cytoplasm of the cell. During glycolysis, glucose is oxidized and converted into two molecules of pyruvate.
This process generates a small amount of energy in the form of ATP and NADH. However, the majority of the energy is still stored in the chemical bonds of the two molecules of pyruvate. After glycolysis, the two molecules of pyruvate are transported into the mitochondria, where they are further oxidized through a process called the citric acid cycle. During this process, more ATP and NADH are generated, and the energy stored in the bonds of the pyruvate molecules is gradually released.
In conclusion, while two molecules of pyruvate do store some potential energy, they do not store as much as one molecule of glucose. This is because glucose has more carbon atoms and more chemical bonds than pyruvate, and therefore has a higher potential energy content.
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Prepare a 500-800 word essay using correct grammar and spelling. Why is there no reason for a cheat meal or day? What is meant by the statement, there are no good foods or bad foods? When people disclose they are craving a food or they emotionally eat, what would be a practical tip or suggestion that might help them address the challenge? What benefits could our society gain by adopting a Health at Every Size approach?
A cheat meal or day is defined as a planned indulgence or reward that enables an individual to indulge in their favorite foods or meals without feeling guilty.
The problem with a cheat meal is that it tends to create negative feelings of guilt, shame, and anxiety for people, especially those struggling with weight loss. There is no reason for a cheat meal or day because it promotes the diet mentality and suggests that there are good and bad foods. However, this is not true because food is neutral, and it is the relationship with food that is either positive or negative. What is meant by the statement, there are no good foods or bad foods? There are no good or bad foods. Foods are not inherently good or bad; they are simply foods.
When we label foods as good or bad, we tend to create an unhealthy relationship with food. For instance, we may restrict ourselves from eating certain foods, which may lead to overeating or binge eating. This is because labeling food creates a sense of morality, which affects the way we think and feel about ourselves. Therefore, it is essential to view food as neutral. When people disclose they are craving a food or they emotionally eat, what would be a practical tip or suggestion that might help them address the challenge? When people disclose they are craving a food or they emotionally eat, it is essential to acknowledge their feelings. One practical suggestion that may help is to identify the emotion that is driving the craving.
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Which variable rises after capillary beds?
a. Blood pressure
b. Blood vessel area
c. blood velocity
d. blood volume
A blood velocity After capillary beds, the blood velocity of the blood rises because of the following reasons. After capillary beds, blood velocity increases. Explanation:Capillaries are the smallest and the thinnest vessels in the human body. These vessels play a vital role in the body as they help in the exchange of various substances such as nutrients, waste, and oxygen. It connects the arterial and venous systems.
The narrowing of the capillaries from tiny vessels increases the resistance of the flow of blood. This then leads to a drop in blood pressure and an increase in blood velocity.The sum of the cross-sectional area of the capillaries is bigger than the cross-sectional area of the arteries and veins; this means that the blood that flows in the capillaries moves at a much slower pace than the blood that flows in the arteries and veins.
This causes a drop in the speed of blood flow, thus leading to a rise in blood velocity.Along with blood velocity, the blood pressure and blood volume may also change after capillary beds. Blood pressure decreases after capillary beds while the blood vessel area and blood volume vary depending on the situation or condition.
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Help
2. What is the advantage of using Sabouraud agar?
The advantage of using Sabouraud agar is it relatively easy to prepare, has higher yield rate than other fungal growth media, will change colour as the pH changes, and can differentiate between fungal species.
Sabouraud agar is a selective and differential medium used for fungal growth, it has a distinct composition with lowered pH that inhibits bacterial growth and improves fungal growth. One of the advantages of using Sabouraud agar is that it's relatively easy to prepare and relatively inexpensive. Secondly, it has a higher yield rate than other fungal growth media, which is why it is commonly used for clinical specimen cultures. Sabouraud agar can also be used for the isolation and identification of fungi from food, water, soil and other materials, this is due to the fact that it has a high degree of sensitivity and specificity.
Another advantage is that the pH indicator of Sabouraud agar will change colour as the pH changes, which makes it easier for the detection of fungal colonies. Lastly, it can differentiate between fungal species by the color and texture of their growth, which helps identify the type of fungal infection a patient has. So therefore the advantage of using Sabouraud agar is it relatively easy to prepare has higher yield rate than other fungal growth media, will change colour as the pH changes, and can differentiate between fungal species.
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A SOAP must always be written in this order: "Subjective,
Objective, Assessment, and Plan".
A. True
B. False
The statement "A SOAP must always be written in this order: "Subjective, Objective, Assessment, and Plan" is A. True
A SOAP (Subjective, Objective, Assessment, Plan) note is a standard format used in medical documentation and patient charting. It is typically organized in that order to provide a logical and structured approach to documenting patient encounters and facilitating communication between healthcare providers.
The subjective section includes the patient's reported symptoms and history, the objective section includes the healthcare provider's observations and objective findings, the assessment section includes the provider's assessment and diagnosis, and the plan section outlines the proposed treatment plan.
Following this order helps ensure consistency and clarity in medical documentation. Therefore, the correct answer is option (A).
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Which of the following is an incorrect statement about "calories"?
a. All one needs to know to accurately calculate one's daily calorie needs is knowledge of their sex and their weight. b. Two hundred calories from an avocado (which offers healthy fats and other nutrients) can be a better choice than eating 100 calories of deli meat. c. Fiber helps to slow the absorption of sugar. d. Healthy eating and drinking choices is about more than calories.
e. A zero-calorie pop/soda, for example, might also provide zero nutrients, and come packed with artificial sweetners. f. Consuming 100 calories in the form of an apple will provide a more "full" feeling than drinking 100 calories of pop/soda/Red Bull, etc.
An incorrect statement about "calories" is: a. All one needs to know to accurately calculate one's daily calorie needs is knowledge of their sex and their weight.
Option (a) is the incorrect statement. While sex and weight are important factors in estimating daily calorie needs, they are not the sole factors. Other variables, such as age, height, activity level, and metabolic rate, also play a significant role in determining individual calorie requirements. Therefore, relying solely on sex and weight to calculate daily calorie needs would not provide an accurate estimation.
Options (b), (c), (d), (e), and (f) are all correct statements about calories. Option (b) highlights that the nutrient content and overall health benefits of food should be considered along with calorie content. Option (c) emphasizes the role of fiber in slowing down sugar absorption, which is important for managing blood sugar levels. Option (d) acknowledges that healthy eating is not solely about calorie intake but also about nutrient quality and overall dietary choices. Option (e) points out that zero-calorie beverages may lack nutrients and contain artificial sweeteners. Option (f) highlights the difference in satiety and fullness between consuming calories from different sources, such as whole foods versus sugary beverages.
Therefore, option (a) is the incorrect statement among the given options.
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What are the infective stage and diagnostic stages for the following diseases?
1. Giardia Lamblia.
2. Leishmania.
3. Ascaris lumbricoides.
4. Toxoplasma Gondi.
5. african trypanosomiasis.
6. Chagas disease.
7. Trichomoniasis Vagainalis.
8. Malaria.
9. Hookworms.
10. Enterobiasis.
11. Entermba Histolatika
1. Giardia Lamblia: Infective stage - Cyst; Diagnostic stage - Trophozoite.
2. Leishmania: Infective stage - Promastigote; Diagnostic stage - Amastigote.
Certainly! Here are the infective stages and diagnostic stages for the mentioned diseases:
1. Giardia Lamblia:
- Infective Stage: Cyst
- Diagnostic Stage: Trophozoite
2. Leishmania:
- Infective Stage: Promastigote
- Diagnostic Stage: Amastigote
3. Ascaris lumbricoides:
- Infective Stage: Eggs
- Diagnostic Stage: Adult worm
4. Toxoplasma gondii:
- Infective Stage: Oocyst
- Diagnostic Stage: Tissue cyst
5. African trypanosomiasis (Sleeping sickness):
- Infective Stage: Metacyclic trypomastigote
- Diagnostic Stage: Bloodstream trypomastigotes, intracellular amastigotes
6. Chagas disease:
- Infective Stage: Trypomastigote
- Diagnostic Stage: Amastigote
7. Trichomoniasis vaginalis:
- Infective Stage: Trophozoite
- Diagnostic Stage: Trophozoite (observed in vaginal secretions or urine)
8. Malaria:
- Infective Stage: Sporozoite
- Diagnostic Stage: Merozoite
9. Hookworms:
- Infective Stage: Infective larvae (L3)
- Diagnostic Stage: Adult worm, eggs in stool
10. Enterobiasis:
- Infective Stage: Infective eggs
- Diagnostic Stage: Adult pinworm or eggs on perianal swab or tape test
11. Entamoeba histolytica:
- Infective Stage: Cyst
- Diagnostic Stage: Trophozoite or cyst in stool sample.
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Deep nucleotide sequencing (NGS) is now cheap enough for general application. What can the technique mainly be useful for?
a. Quickly identifying new viruses
b. O speedy vaccine development
c. giving details on virus excretion in symptomless carriers
d. establishing the reproductive number of a virus
NGS (deep nucleotide sequencing) can mainly be useful for:
a. Quickly identifying new viruses.
c. Giving details on virus excretion in symptomless carriers.
A virus is an infectious submicroscopic creature that only reproduces inside of live cells. All living things, including plants, animals, and microbes like bacteria and archaea, are susceptible to virus infection. More than 11,000 of the millions of viral species have been characterised in detail since Dmitri Ivanovsky's 1892 publication revealing a non-bacterial disease infecting tobacco plants and Martinus Beijerinck's discovery of the tobacco mosaic virus in 1898. Viruses are the most common sort of living organism and may be found in practically all ecosystems on Earth. Virology is the study of viruses; it is a branch of microbiology.
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If crossing over occurred in one cell and not another, how would the two cells compare?
A. Crossing over would have no effect on either cell.
B. Crossing over would increase the genetic diversity in one of the cells.
C. Crossing over would increase the chromosome number in one of the cells.
D. Crossing over would decrease the chromosome number in one of the cells.
E. Crossing over would cause one of the cells to stop dividing.
If crossing over occurred in one cell and not another, the two cells would have different genetic compositions.
Option B, "Crossing over would increase the genetic diversity in one of the cells," is the correct answer. Crossing over introduces new combinations of alleles by shuffling genetic material between the homologous chromosomes. This process promotes genetic diversity in offspring, as it creates novel combinations of genes that were not present in the parent cells. Options A, C, D, and E are not accurate in this context. Crossing over does have an effect on cell genetics, it does not affect the chromosome number, it does not decrease the chromosome number, and it does not cause one of the cells to stop dividing.
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All the following about telomerase and telomeres are true except: there is no telomerase activity in somatic cells. the telomerase enzyme carries an RNA template. telomerase adds RNA nucleotides to the lagging strand. O telomere length and age-at-death appear to be correlated. human telomeres are repeats of 6 nucleotides. Question 10 1 pts Which of the following statements about DNA replication is correct? Does not require a primer to replicate the leading strand. The lagging strand is synthesised discontinuously in the 5' to 3' direction. Proceeds in one direction from the origin of replication. Topoisomerase unwinds and separates the parental DNA strands. O Uses DNA ligase to form the H-bonds holding the two complementary strands together.
28. In Chapter 12, we discussed different tests/assays we could use to identify the microbe(s) causing an infection in a host. Please describe one of these methods (or use your own example) and report (1 sentence/term):
a) The name of the method and how it works:
b) A type of control sample (either positive or negative control) you can run with your sample:
c) A false positive or false negative result that could occur when you run each assay:
The method described is Polymerase Chain Reaction (PCR), a molecular technique used to amplify specific DNA sequences. PCR involves a series of temperature cycles that allow DNA replication to occur in vitro.
It starts with denaturation, where the DNA strands are separated by heating. Then, primers specific to the target DNA sequence are annealed to the separated strands. Next, DNA polymerase extends the primers, synthesizing new DNA strands. This process of denaturation, annealing, and extension is repeated multiple times, resulting in the exponential amplification of the target DNA sequence if present.
A positive control sample that can be run alongside the test sample is a known sample containing the target DNA sequence of the microbe being tested. This positive control should yield a positive result, confirming that the PCR assay is working correctly and capable of detecting the target DNA sequence.
False positive results in PCR can occur if there is contamination in the laboratory. Contaminating DNA, such as stray DNA from previous experiments or reagents, can be amplified, leading to a positive signal even in the absence of the target microbe. False negatives, on the other hand, can occur if the primers used in the PCR assay do not match the DNA sequence of the microbe causing the infection. If the primers fail to bind to the target DNA, amplification will not occur, resulting in a negative result despite the presence of the microbe.
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Tachycardia with marked rise of blood pressure occur after administration of : Select one O a. Isoprenaline b. Noradrenaline O c. Dobutamine d. Adrenaline d e. Atropine
Tachycardia with a marked rise in blood pressure is observed when adrenaline is administered. Adrenaline is a naturally occurring hormone, also known as epinephrine.
That plays a vital role in the fight or flight response in the body.Tachycardia, an abnormally high heart rate, and hypertension, or high blood pressure, are among the possible side effects of the drug administration. Adrenaline is a medication that is often used in emergency situations.
The medication is administered by injection, and the dosage is determined by the patient's weight, the severity of their condition, and other factors. The medication stimulates beta receptors in the body, resulting in increased heart rate and constriction of blood vessels.
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When pyrimidines undergo catabolism the result is: Pyrimidines are eventually broken down into ammonia and eliminated as nitrogenous waste or reused in purine synthesis Production and elimination of uric acid Production of malonyl-CoA which is then reused in fatty acid and polyketide Synthesis. Production of chorismic acid and integration into polyketide synthesis
The correct answer is 1. Pyrimidines are eventually broken down into ammonia and eliminated as nitrogenous waste or reused in purine synthesis.
Pyrimidines are broken down by a series of enzymes into ammonia, carbon dioxide, and β-alanine. The ammonia can be used to synthesize new pyrimidines, or it can be excreted as a waste product.
The other options are incorrect.
Uric acid is a product of purine catabolism, not pyrimidine catabolism.
Malonyl-CoA is not produced from pyrimidine catabolism. It is produced from acetyl-CoA in the fatty acid synthesis pathway.
Chorismic acid is not produced from pyrimidine catabolism. It is produced from the amino acid tryptophan in the biosynthesis of aromatic amino acids, including phenylalanine, tyrosine, and tryptophan.
Therefore, (1) Pyrimidines are eventually broken down into ammonia and eliminated as nitrogenous waste or reused in purine synthesis is the correct option.
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1. In both the male and female cath, identify structures that hure a urogcritil function. a. Male cat b. Fernale cat QUESTIONS 2-11: Match the cat teproductive structure in columin A with the apeopeia
In the male cat the urethra and the pe.nis have a urogenital function, while in the female cat, the va.gina has a urogenital function.
What organs have a urogenital structure?Organs have a urogenital structure if they are important for both the reproductive and the urinary systems:
The urethra, and pe.nis: These organs are important for the transportation of spermatozoids in the reproductive system but also for the transportation of urine.Va.gina: Similar to the urethra this structure has a reproductive purpose but also allows the urine to be transported out of the body.Note: Here is the complete question:
In both the male and female cath, identify structures that have a urogenital function.
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Cell wall inhibiting antibiotics impair ______ Multiple Cholce
a. binary fission
b. cellular respiration c. feranentation
Cell wall inhibiting antibiotics impair binary fission in bacterial cells. Cell wall inhibiting antibiotics, such as penicillin and cephalosporins, target and interfere with the synthesis of peptidoglycan, a crucial component of the bacterial cell wall.
The cell wall provides structural support and protection to the bacterial cell. Binary fission is the process of bacterial cell division where one parent cell divides into two identical daughter cells. During binary fission, the bacterial cell elongates, replicates its DNA, and then forms a septum dividing the cell into two separate cells. The formation of a new cell wall is a critical step in the binary fission process.
By inhibiting the synthesis of peptidoglycan and disrupting cell wall formation, cell wall inhibiting antibiotics impair the process of binary fission in bacterial cells. This hinders the ability of bacterial cells to divide and multiply, ultimately inhibiting their growth and causing the bacteria to be more susceptible to immune responses or other antimicrobial treatments.
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9. Which of the following sunlight classes of UV radiation has the shortest wavelength? a) UVA b) UVB c) UVC d) UVD 10. Human Papillomavirus is the main cause of _____.
a) testicular cancer b) cervical cancer c) breast cancer d) hepatocarcinoma 11. The phenomena in which the integration of viral DNA into host chromosome that cause activation or disruption of a normal gene is known as ______.
a) insertional mutagenesis b) proliferating mutagenesis c) transforming mutagenesis d) constitutive mutagenesis
UVC has the shortest wavelength among the sunlight classes of UV radiation. Cervical cancer is mainly caused by Human Papillomavirus (HPV). The underlying mechanism is thought to be insertional mutagenesis, a process by which there is an integration of viral DNA into the host chromosome that causes activation or disruption of the normal host gene.
Among the sunlight classes of UV radiation, UVC has the shortest wavelength. UVA has the longest wavelength, followed by UVB and then UVC.
Human Papillomavirus (HPV) is the man causative agent of cervical cancer. HPV is a sexually transmitted infection that can lead to the development of abnormal cervical cells, which, if left untreated, can progress to cervical cancer. It is important for individuals, particularly females, to undergo regular screening tests, such as Pap smears and HPV testing, to detect and prevent cervical cancer.
The phenomenon in which viral DNA integrates into the host chromosome and causes activation or disruption of a normal gene is known as insertional mutagenesis. Viral DNA can insert itself into the host genome and affect the expression and function of genes.
This integration can lead to genetic changes that contribute to the development of various diseases, including certain types of cancer. Insertional mutagenesis is a mechanism through which viruses can alter the normal functioning of host cells and potentially drive cellular transformation.
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You are conducting a research project on bacteriophages and have been culturing a bacterial host in the presence of its targeting phage. After exposing the host to a phage for several generations you plate the culture and isolate a bacterial colony. You then culture this colony, make a lawn with this culture, and spot your phage stock on the surface. The next day, you observe that there are no plaques on the lawn. What would you conclude from this result? The phage has mutated to be ineffective on the bacterial host O The phage is temperate/lysogenic The bacterial isolate is a phage resistant mutant The top agar is interfering with phage absorption The bacterial isolate is susceptible to antibiotics
From the observation of the researcher where no plaques have been observed on the lawn, we can conclude that the bacterial isolate is a phage resistant mutant . What are bacteriophages? Bacteriophages are viruses that affect bacteria . They are specific to a particular type of bacteria.
Phages attach themselves to the bacteria and inject their genetic material into it. This can lead to the death of the bacterium. Bacteriophages have a wide range of potential uses, including the treatment of bacterial infections. In a research project on bacteriophages, if after exposing the host to a phage for several generations, no plaques are observed on the lawn, it means that the bacterial isolate is a phage resistant mutant.
Option 1: If the phage had mutated to be ineffective on the bacterial host, then no colonies of bacterial host would have grown in the culture.Option 2: If the phage were temperate/lysogenic, the phage would have integrated its genome into the bacterial chromosome, and the bacterial colony would have displayed turbidity or changed its colony morphology, but no plaques would have been seen on the lawn.Option 3: The bacterial isolate being a phage-resistant mutant is the correct answer.Option 4: The top agar is interfering with phage absorption, which may cause a problem in seeing the plaques in the lawn.Option 5: The susceptibility of bacteria to antibiotics is unrelated to the bacteriophages. Therefore, it is not an answer to this question.
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1. Which biological science is the one that I told you is a complex subject ke immunology? & biochemistry A. microbiology B. biochemistry
D. biophysics C. neuroscience E. cell biology 2. Mucous membranes are a part of the_____________
A. adaptive immune system B. lymph node C. physical barrier D. bone marrow E. Ethymus
1. The biological science that is as complex as immunology is: B. Biochemistry
Immunology is the study of the immune system and how it works to fight off pathogens. Biochemistry is the study of chemical processes and substances in living organisms. Both fields can be quite complex, but biochemistry can be just as complex as immunology.
2. Mucous membranes are a part of the: C. physical barrier
Mucous membranes are a type of physical barrier in the body's defense against infection. They line various organs and body cavities, such as the nose, mouth, throat, lungs, and reproductive organs. The mucus produced by these membranes helps trap pathogens and prevent them from entering the body.
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