Answer:
b. A second marker in the knock-out cassette, that if inserted into the genome results in cell death when plated on selective media.
Explanation:
General recombination, also known as homologous recombination, refers to the naturally occurring process of exchange of genetic material between pairs of homologous DNA sequences. This process (homologous recombination) can be exploited by genetic engineering to insert DNA segments of interest at target genes. Moreover, a cassette is a mobile DNA segment containing almost a gene and a recombination site, which is integrated into the locus/loci of interest by homologous recombination. A cassette may contain a DNA segment called 'negative marker' which prevents growth under particular conditions, while a positive marker permits growth under certain conditions. In consequence, a second marker consisting of a drug cassette may be used as a negative marker in order to evidence its insertion by inducing cell death when they are plated in selective conditions.
c. Challenge: You are working with a neuronal cell line isolated from a human brain tumor. It appears to have a neuronal phenotype when grown in culture on laminin coated dishes but appears to have more of a typical round, small (3 to 5 micrometer) phenotype when grown on a standard, collagen-only coated dish or a poly-lysine coated (positive charge only - no ECM component) dish. Under all 3 cases the cells attach to the substrate. You suspect that the reason the neuronal phenotype is supported by the laminin coating is due to the ability of laminin, but not collagen or poly-lysine, to bind to the integrin receptors RGD binding domain thus triggering a molecular cascade that leads to the expression of the neuronal phenotype. How might you test this hypothesis
Answer:
This process can be performed using immunofluorescence analysis via antibodies.
Explanation:
To test for the hypothesis
To test for the hypothesis we have to analyze whether the neuronal phenotype is due to the binding of laminin to integrins, and this will enable us to determine which integrins are abundantly expressed on the neuronal cell membrane. This process can be performed using immunofluorescence analysis via antibodies
Finally when no differentiation markers are expressed in the laminin coated plates with mutant cells we can now say that it expresses integrins
Put the following events in order.
The junctional fold of the motor end plate
must be depolarized and a wave of
depolarization travels along the sarcolemma
[Choose ]
[Choose)
The transverse tubules carry the depolarizing
current to the interior of the muscle cell where
they activate voltage-sensitive channels to
change shape.
[Choose
This shape change opens calcium-release
channels in the terminal cisterns of the
sarcoplasmic reticulum.
Calcium floods into the sarcoplasm
[Choose ]
Testosterone does which of the following?
OA. Develops accessory sex organs.
ОВ.
AIDS in spermatogenesis.
OC. Controls sex drive.
OD
All of the above
PLEASE HELP MEEE I NEED HELP ON THIS QUESTION
Answer:
C. Spore formation
Explanation:
Option C above is the correct answer.
Spore formation is actually a method of asexual reproduction. Fungi actually reproduce asexually via spore formation. Some examples of organisms that reproduce via spore formation are Rhizopus, mushroom, mucor, etc.
The spores are usually stored in sacs which are known as sporangia. When there is a bursting of the sac, spores that are single-celled, thick and thin walled are obtained/formed. The spores are usually resistant to environmental conditions.
NEED ANSWERED ASAP
A three letter base sequence on a mRNA molecule called a _____ translates for a particular amino acid.
Answer:
codon
Explanation:
Polio viruses contain DNA. The viruses in the polio vaccine have DNA in which the base sequence has several differences from the base sequence in the normal polio virus. Suggest how this makes them unable to reproduce in the human body.
Answer:
The polio strains cannot reproduce because they are weakened.
All living cell's are composed of?
Answer:
All cells are made from the same major classes of organic molecules: nucleic acids, proteins, carbohydrates, and lipids.
Explanation:
All cells are made from the same major classes of organic molecules: nucleic acids, proteins, carbohydrates, and lipids.
10 time asking because of link spammers.
What are the three parts to a feedback loop?
Select one:
Positive, negative, and neutral pathways.
Receptors, producers, and transmitters.
Stimulus, sensor, and response.
Input, output, and direction of signal.
Answer:
Stimulus, sensor, and response.
Which of the following best defines a food web?
Answer:
A chain of organisms in which each link feeds on the one ahead to gain energy.
Answer: A food web consists of all the food chains in a single ecosystem. ... Each food chain is one possible path that energy and nutrients may take as they move through the ecosystem. All of the interconnected and overlapping food chains in an ecosystem make up a food web.
Explanation:
Organisms use phosphorus to help maintain homeostasis Phosphorus is a building block of molecules that function in making
organisms.
3. Which of the following statements is not part of modern cell theory? A. All living things are made up of multiple cells. B. All living organisms are made up of cells. C. Cells are the basic building blocks of life. D. All cells come from pre-existing cells.
Answer:
A. All living things are made of multiple cells.
The definition of a parasitic relationship is what
Answer:
A parasitic relationship is one in which one organism, the parasite, live off of another organism, the host, harming it.
The skeleton of the human hand is similar in structure to
a. Bird's wings
b. Ancient fish
c. Dog's legs
d. All of the above
Answer:
a. Bird's wings
Explanation:
You may assume the Sickle Cell locus is in Hardy Weinberg equilibrium. Also, the term carrier implies that the person has a particular allele in his/her genotype, but is not affected with the condition. If the proportion of an African population that are susceptible to Malaria is 0.688, what proportion of the population are carriers for the Sickle Cell allele (HbS)
Answer:
The frequency of the population that are carriers is 0.284 or 28.4%
Explanation:
According to Hardy-Weinberg equilibrium equation, p² + q² + 2pq = 1
Also, the sum of the allele frequencies for all the alleles at the locus, p + q = 1.
Where where p is the frequency of the dominant allele and q is the frequency of the recessive allele in the population.
p² = dominant h0m0zygous genotype frequency
2pq = heterozygous genotype frequency
q² = recessive h0m0zygous genotype frequency
From the given data; the dominant h0m0zygous genotype, i.e., the proportion of an African population that are susceptible to Malaria, p² = 0.688
p = √0.6880 = 0.829
But p + q = 1; q = 1 - p
Since p = 0.829, q = 1 - 0.829
q = 0.171
Frequency carriers for Sickle Cell allele, i.e., the heterozygous genotype = 2pq
Frequency of carriers = 2 × 0.829 × 0.171 = 0.284
Therefore, the frequency of the population that are carriers is 0.284 or 28.4%
Which of the following refers to the diversity of available genes within a population?
A)
demographics
B)
genetic variety
C)
gene pool
D)
population distribution
E)
tolerance range
Q1: what is sleet?
Q2: how big can raindrops get?
Q1. It is a form of precipitation that consists of ice pellets often mixed with rain or snow. it is usually soft and contains some traces of ice. It can be dangerous to drive with this on the road.
Q2: They can get as big as 4 millimeters. rain drops don't usually get any bigger without seperating into two droplets.
Explanation:
(I hope this helps! Have a great day!)
A small proportion of humans have resistance to strains of HIV, the virus that causes AIDS. Their immunity is caused by a deletion in gene CCR5, a co-receptor on white blood cells needed for HIV infection. The proportion of people with resistance (genotype aa) to HIV is approximately 8%. If these alleles are in Hardy Weinberg equilibrium, what proportion of people are carriers for the HIV resistant allele?
Answer:
The correct answer is "40.6%". A further explanation is provided below.
Explanation:
The given values are:
The proportion of people with resistance is,
aa = 8%
or,
= 0.08 (q²)
q² = 0.08
q = √0.08
= 0.28284
Now,
⇒ [tex]p+q=1[/tex]
⇒ [tex]p=1-q[/tex]
[tex]=1-0.28284[/tex]
[tex]=0.717157[/tex]
hence,
For the HIV resistant allele, the proportion of people will be:
= [tex]Aa[/tex]
= [tex]2pq[/tex]
On substituting the values, we get
= [tex]2\times 0.71715\times 0.28284[/tex]
= [tex]0.4056[/tex]
= [tex]40.6[/tex] (%)
The proportion of the population with the carrier of HIV-resistant allele is 40.56%.
What is Hardy Weinberg equilibrium?
Hardy Weinberg equilibrium is the relationship between the variation from one generation to the next. The equation for the relationship can be given as:
[tex]p^2+2pq+q^2=1[/tex]
Where the proportion of dominant alleles is p².
The proportion of recessive alleles is q².
The proportion with the caries are 2pq.
The given recessive allele proportion in the population is 0.08. The proportion of dominant alleles can be given as:
[tex]q^2=0.08\\q=0.2828[/tex]
The dominant alleles can be given as:
[tex]p+q=1\\p+0.28284=1\\p=0.717157[/tex]
The carriers in the population can be given as:
[tex]\text{Carriers}=2pq\\\text{Carriers}=2\;\times\;0.717157\;\times\;0.2828\\\text{Carriers}=0.4056\\\text{Carriers}=40.56\%[/tex]
Thus, the carriers for the HIV gene in the population are 40.56%.
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What is species evenness?
a. The distribution of species throughout a habitat
b. The number of different populations in a community
c. The relative abundance of species in a community
The relative abundance of species in a community is known as species evenness. Therefore, option C is correct.
What is species evenness?Species evenness refers to the measure of the relative abundance of different species within a community or ecosystem. It describes the balance of the distribution of individuals among the different species present in a particular area.
For example, a community with high species evenness would have similar numbers of individuals in each species, while a community with low species evenness would have a few dominant species with many individuals and many less abundant species with fewer individuals.
Species evenness is often used in conjunction with species richness, which refers to the total number of different species present in a community, to provide a more complete picture of the biodiversity of an area. Thus, option C is correct.
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Select the correct answer.
Which is an example of dehydration synthesis?
A. DNA synthesis from nucleotides
B. conversion of proteins into its amino acids
C. conversion of lipids into fatty acids
On the planet Seltaeb, there is a rare recessive condition known as Hofner Bassis (BB) in the Blue Meanies, a race whose genomes are identical to that of humans. Individuals with this condition can be clearly identified, as they are very musically gifted. The two alleles at this locus are B (the trait allele) and b. If both parents are known to be carriers for the recessive gene, what is the probability that they will have an affected child
Answer:
The probability that they will have an affected child is 25%
Explanation:
Hofner Bassis (BB) is recessive gene and is expressed in an individual who have bb genotype.
Two carrier parents will have genotype = Bb
Bb * Bb
BB, Bb, Bb, bb
The probability that they will have an affected child is 1/4 = 25%
The Arg genes that encode the enzymes for arginine biosynthesis are located at several positions around the genome of E.coli, and they are regulated coordinately by a transcription regulator encoded by the ArgR gene. The activity of the Arg R protein is modulated by arginine. Upon binding arginine, ArgR alters its conformation, dramatically changing its affinity for the DNA sequences in the promoters of the genes for the arginine biosynthesis. A. Given that ArgR is a repressor protein, would you expect that ArgR would bind more tightly or less tightly to the DNA sequences when arginine is absent?
Solution :
The Arg genes is used to synthesize the arginine. Arginine is chemical building block. It is very important and is used by our body to make the proteins for our body. When the Arg ID is abundant, the expression for the biosynthetic genes must turned off. And if the ArgR behaves as the gene repressor, then the binding of the arginine increases the infinity for the regulatory sites, and allows it to the bind and then shut off the expression of the genes.
If the ArgR acts as the gene activator, then the binding of the arginine would be predicted to reduce the affinity for the regulatory DNA, and preventing the binding and the shutting off the expression for the Arg genes.
Which statement correctly describes the flow of energy in photosynthesis?
Answer:
DPhotosynthesis stores energy because energy is released when glucose is formed from carbon dioxide and water. The passage describes a condition that is inherited.
Energy from the Sun flows into plants through photosynthesis, which is also known as “putting together with light,” and eventually into humankind. In the end, humans are powered by the Sun's energy to eat and survive. Thus, option D is correct.
What describes the flow of energy in photosynthesis?Solar energy is converted into chemical energy during photosynthesis, which produces glucose from water and carbon dioxide. The by-product is the emission of oxygen. In the process of breaking down glucose during cellular respiration, oxygen is consumed, which also produces heat and chemical energy.
Photosynthesis is the tremendous energy flow that underlies electricity and is the foundation of all life. Whether we are vegetarians or not, we joyfully devour our food without giving the basic mechanism that provides us with this gift even the least thought.
Therefore, Because energy is released when glucose is created from carbon dioxide and water, photosynthesis saves energy. The paragraph mentions a hereditary ailment.
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which is the main chemical substance found in khat?
Answer:
The principal active components in khat are cathinone and cathine (norpseudoephedrine) (see also Drug profile on synthetic cathinones). Chewing khat releases these substances into the saliva; they are rapidly absorbed and eliminated.
Explanation:
As much as 25% of the fruits and vegetables grown in Chinese agricultural
regions rot before they can be brought to market. Which of these approaches
is a possible solution?
A. More-efficient preservation methods
B. A reduction in irrigation
C. Planting more seeds
D. Less-efficient ways of farming
Answer:
A.More-efficient preservation methods
What is the function of the placenta?
A. To protect the developing fetus
B. To help the developing fetus communicate with the mother
C. To hold the developing fetus in place
D. To provide the developing fetus with food, water, and oxygen
Answer:
it's either a or d I know this I'm in child development but I'm pretty sure it's a d but it might help protect the fetus because it's trying to keep it attached and keep it from floating around but it could be d because to provide developing food water and oxygen that would be the only way it could breathe and that's how it gets his nutrients because when the mother is breathing in oxygen and eating that's how the baby gets to take in all that stuff with the mother
To provide the developing fetus with food, water, and oxygen is the function of placenta. Therefore, option (D) is correct.
What do you mean by placenta?An organ that grows inside the uterus during pregnancy is the placenta. A developing infant receives oxygen and nutrients from this structure.
As your womb gets smaller, your placenta will fall out and the blood vessels that were holding onto it will close. This assists with forestalling a lot dying - despite the fact that draining a little is ordinary.
The placenta is the membrane that the mother and fetus use to exchange gases, nutrients, and other things. Syncytiotrophoblast, cytotrophoblast, embryonic connective tissue, and the endothelium of fetal blood vessels all contribute to the formation of the membrane.
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Please accurately define each term and explain the role of each term in the scenario below Phillip has been preparing to give the valedictorian speech at his high school since mid-February. He has a fear of public speaking that began in elementary school when he forgot a speech when he was running for student council president. He has been working with the drama teacher at the school to overcome this fear. As graduation approaches, he has started to have difficulty sleeping and has lost his appetite. He has worked hard to earn the opportunity and does think his message of finding his way as a child of immigrants and divorce is important. Still he is beginning to question whether or not he will be able to present the speech in front of such a large crowd. He has spoken to his guidance counselor about his concerns. Explain how each of the following concepts relates to the scenario:
. Phobia .
Sympathetic nervous system
• Intrinsic motivation
• Positive reinforcement
• Extraversion
• Altruism Elaborative rehearsal
Answer:
See explanation below
Explanation:
Phobia: Phobia refers to an extreme and irrational fear of something. It can also be an aversion to something. Philip has a fear of public speaking that began when he forgot a speech in elementary school.
Sympathetic Nervous System: This is one of the response to stressful situations. It is activated by the hypothalamus due to both physical and psychological stress. It directs the body’s rapid involuntary response to dangerous or stressful situations. As graduation approaches, Philip starts to have difficulty sleeping and loss of appetite.
Intrinsic Motivation: This refers to doing something just because it is enjoyable rather than for any external reward. Prior to the graduation, Philip has been working with his drama teacher to help him overcome his fear of public speaking.
Positive Reinforcement: This is a form of reward for performing a task. People can also seek positive reinforcement before they perform an action just so that they perform well. Philip tried to get reinforcement his guidance counselor before his presentation.
Extroversion: This is the inclination to obtain gratification from outside oneself. By questioning whether he would be able to present his speech and impress a large crowd, Philip is trying to obtain gratification from others.
Altruism: This refers to a selfless concern for the happiness and well-being of others. Philip is concerned about how his message would impact others who may be able to learn from his journey of being a child of divorce and immigration.
Elaborating Rehearsal: This is done by helping ones short term memory to store information in ones long term memory. Philip has been preparing to give the valedictorian speech at his high school.
In what form is energy stored in muscles
In the process of depletion, the soil loses the surface soil, which contains most of the organic matter.
True
False
When blood passes through a capillary bed in the
it downloards O2 and uploads CO2.
Answer:
Exchange of gases takes place at alveoli sacs of lungs, which are equipped with capillary vessels or bed.
Explanation:
On inspiration or taking air through nostrils, surrounding air enters the lungs and it reaches air sacs or alveoli. Here, the outside air will have more amount of oxygen when compared with oxygen in blood at that site. Hemoglobin in the blood have high bonding capability to oxygen, so it captures oxygen and takes it to heart for pumping into other parts of the body. Likewise, the circulations continues from lungs to heart > hear to body parts > body parts to heart > heart to lungs for purification.
What is the right answer
Answer: Full Moon
Explanation: The Earth blocks the sunlight radiated for the Moon, so parts of the moon would be shadowed by the earth as seen by people. You do actually, see the entire phase of the round ball during a Full Moon. It is during when the Earth's orbital plane is inclined, or angled, by a few degrees from the ecliptic plane, meaning the Earth doesn’t block its sunlight during this time.
Hope it helps! You can refer to the website for more: https://www.quora.com/Why-do-we-see-a-%E2%80%98full-Moon%E2%80%99-if-the-Earth-blocks-the-light-from-the-Sun