How can you guarantee a high expression of your protein in any expression vector?

Answers

Answer 1

Answer:

protein expression refers to the way in which protein are synthesized, modified and regulated in living organisms.

Explanation:

In the protein research,the term can apply either the object of study or the laboratory techniques required to manufacture proteins.

How Can You Guarantee A High Expression Of Your Protein In Any Expression Vector?

Related Questions

How many more phases occur during meiosis than during mitosis?
none
two
four
six

Answers

Answer:

there are 4 more phases that occur during meiosis than mitosis

Answer:

The answer is.....

Explanation:

C. Four

Where is the last storage location for melted snow? (2 points) Where is the last storage location for melted snow? (2 points) Group of answer choices Rivers Ocean Streams Mountains

Answers

Answer:

Ocean

Explanation:

Ice is usually found in the glaciers in the mountains and once it gets heated it flows down through melting and forms a stream and enters into rivers and these streams combine and lead to the oceans. Thus these oceans become the ultimate storage units of melted snow. Ice and cannot stay in solid-state for too long.

Which of the following could be discovered by studying the gross anatomy of a cadaver?
The function of cells within the body
The types of cells within each organ
The function of the body's organs and systems
The structure of the body's organs and systems

Answers

Answer:

The structure of the body's organs and systems

Explanation:

In medicine, a cadaver is a dead human body dissected in order to study its anatomical structure. It is employed by medical students and other medical practitioners to study the structure of the human body.

Anatomy is a branch of science that has to do with the study of the structure of living organisms. Relating anatomy to cadaver, these dead bodies are dissected in order to view and study the structure of the different compartments of the body e.g organs, systems etc.

Explain why chemogenetic based methods of regulating cellular signaling result in poor temporal control of biochemical pathways relative to optogenetic based methods.

Answers

Answer:

They use different detecting methods to assess neuronal circuits

Explanation:

Chemogenetics is a technique widely used in neuroscience research to explore signaling interactions by means of genetically modified receptors capable of interacting with small molecules. Chemogenetics was first used to determine the function of the chalcone isomerase gene by inducing mutations that altered its substrate specificity. On the other hand, optogenetics is a bioluminescence-driven genetic technique used to control genetically modified neurons that express light-gated ion channel genes. This technique is also used to monitor neuronal networks. In consequence, optogenetic and chemogenetic techniques have recently been combined in order to analyze neuronal circuits, it by analyzing the same actuator molecule.

Please help me come up with an answer for this question thankyou so much! Compare and contrast the processes of cellular respiration and photosynthesis. Think of as many similarities and differences as you can (things to consider: inputs and outputs, the role of energy, the types of organisms that do each process, the location within cells)

Answers

Explanation:

The end result is glucose, with oxygen being the waste product. The two processes are similar in that they both produce energy, albeit in two different forms. They are different in that photosynthesis assembles the glucose molecule, while cellular respiration takes it apart.

Which of these lymphoid organs destroys bacteria before it can breach the intestinal wall and generates "memory" lymphocytes for long-term memory?

Answers

Answer:

Peyers patches

Explanation:

Peyers patches are group of lymphoid follicles found in the ileum of the small intestine.

This part of lymphatic system form part of immune system that help to control bacteria population in the small intestine and prevent the growth of pathogenic bacteria in the small intestines. It plays an important role in immune surveillance where it recognises pathogenic bacteria and destroy the bacteria.

It is a lymphoid organs destroys bacteria before it can breach the intestinal wall and generates "memory" lymphocytes for long-term memory because it form the part of the immune system which help to fight infection or foreign substances in the body, also by generating lymphocytes which is a white blood cells of the immune system which generate long term memory of foreign pathogens and destroying it.

help asap plss giving branlist

Answers

Answer:

Parasitic Relationship

Explanation:

Symbiotic relationship:

is a term that defines an interaction between organisms of different organisms.

Parasitic Relationship:

A relationship in which one organism is benefited and one is not.

Example: A tapeworm living at the gastrointestinal parts.

Mutualistic Relationship:

A relationship in which both organisms benefit.

Example: Egyptian plover bird and the crocodile.

Commensal Relationship:

A relationship in which both organisms are not benefited nor harmed.

Hope this helps ;) ❤❤❤

The uterine cycle describes the cyclic changes of thickening and degeneration that the endometrium goes through in a month. What is the order of events in one uterine cycle

Answers

Answer:

During the uterine month there are different phases through which the uterus passes, these phases are regulated by hormones and are responsible for producing the cycle necessary for fertilization.

Phase where menstruation occurs: This phase only happens if the woman was not fertilized and did not develop the diploid cell together with a sperm, since not being fertilized, all the uterine preparation that had been planned in the body for fertilization will be released as that we know "menstruation", in this phase estrogens and progesterone are low. The inner walls of the unfertilized uterus are released.

Follicular phase, in the follicular phase the ovaries prepare to release an egg and estrogen begins to rise. (From the first day of the period until ovulation)

Proliferative phase, in the proliferative phase, new vessels proliferate and the outermost layer of the uterus prepares itself for possible fertilization, is where spiral arterioles can begin to form again in the external cut of the myometrium.

Ovulation, here is where the mature ovum is called Graff's follicle, at this time estrogen reaches its peak and then descends.

Luteal phase, in the luteal phase the production of the luteal body is generated, at this stage progesterone takes center stage, and it is the range between ovulation and menstruation (if not fertilized)

Last phase, secret phase, in this phase there are two possible ends, if the woman is fertilized, the egg cell implants and begins the development of the embryo and if it is not fertilized, the entire external cut of the myometrium is prepared to be secreted.

Explanation:

A very important fact to clarify is that women are born with a quantity of ovules that at the end of this uterine cycle ceases to exist, this process is what we know as menopause.

That is to say that women have a quantity of ovules that will one day run out, and the body releases them from the menarche or the first menstruation, generating that in each released ovule a uterine cycle is completed, the day they end the woman will have reached menopause and would have no chance of being fertilized or completing the uterine cycle.

superficial layer of the endometrium is shed

basal layer of endometrium grows, forms gland and blood vessels

enriched endometrial blood supply

endometrial glands secrete nutrients into uterus

Which type of organism developed first?

Answers

al

answer: algae

explanation: because the were the first ones to adapt with water and land...

21. Evidence in support of the importance of endosymbiosis in the evolution of
eukaryotic cells is:
a, Organelles that have their own unique unique DNA and ribosomes
Organelles that have a double membrane
6. Organelles are in the same size range as free living prokaryotes
d. All of the above

Answers

Answer:

d. All of the above

Explanation:

The theory of endosymbiosis is important as it explains the evolution of  eukaryotic cells from prokaryotic cells.

This theory also explains the origin of chloroplast and mitochondria. Several evidences in support of the importance of endosymbiosis in the evolution of  eukaryotic cells are as following:

Organelles have their own separate DNA and ribososme.Organelles that have a double membrane  (inner and outer).Organelles are in the same size range as free-living prokaryotes.

Chloroplast and mitochondria are the examples of such organelles.

Hence, the correct answer is "d".

A pharmaceutical company is working on a drug to slow the spread of Ebola. Which of the following should be the company's focus?

a. Sanitizing the drinking water
b. Protecting the food supply
c. Person-to-person contact
d. Killing the insect vector

Answers

Answer:

C

Explanation:

Although Ebola isn't transferred by the air and through touch. Bodily fluids like sweat, saliva, urine, breastmilk, semen, and vomit all transmit the disease, if a person gets it in a cut or is directly introduced by it (sexual intercourse.)

Below are two chemical equations. One is for cellular respiration and one is for photosynthesis. Correctly place the reactants and products in the right location to complete both equations.
Photosynthesis: H20 +H20------------->C6H12O6 +O2
+solar energy CO2
Cellular Respiration:O2+C6H1206 -------->CO2 + H2O
+02

Answers

Answer:

Photosynthesis: 6CO₂ + 6H₂O + solar energy ----> C₆H₁₂O₆ + 6O₂

Cellular Respiration: C₆H₁₂O₆ + 6O₂ ----> 6CO₂ + 6H₂O + ATP

Hope that helps.

The correct equation for cellular respiration and one for photosynthesis are:

Photosynthesis: 6CO₂ + 6H₂O + solar energy ----> C₆H₁₂O₆ + 6O₂

Cellular Respiration: C₆H₁₂O₆ + 6O₂ ----> 6CO₂ + 6H₂O + ATP

What is cellular respiration?

Cellular respiration is a process of converting food into energy that can be used in many processes. It happens when oxygen is combined with food material and releases carbon dioxide as a waste product.

The correct reaction of photosynthesis is when carbon dioxide and water are combined with sunlight to form glucose and oxygen.

The correct reaction of cellular respiration is when glucose and oxygen react and release carbon dioxide and water and energy,

Thus, the correct reactions are:

Photosynthesis: 6CO₂ + 6H₂O + solar energy ----> C₆H₁₂O₆ + 6O₂

Cellular Respiration: C₆H₁₂O₆ + 6O₂ ----> 6CO₂ + 6H₂O + ATP

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what is the importance of autotrophic organisms for other organisms?

Answers

Answer:

the importance of autotrophic organisms with respect to energy flow and nutrient cycling in ecosystems. Primary producers usel solar or chemical energy to manufacture their own food and they use this energy through cellular respiration and growth and reproduction--energy used for NPP.

14. Which of the following may produce more than one functional protein
from an mRNA transcript?
a. chromatin condensation c. epigenetics
h transcrintional regulation d. alternative mRNA processing

Answers

Answer:

D. Alternative mRNA processing.

Explanation:

The iris in the human eye contracts and expands, controlling the amount of light that reaches the retina. What types of muscle control this?To which skeletal system does the sternum belong?

Answers

Answer:

1. sphincter muscle and dilator muscle

2. Axial skeleton

Explanation:

1.The iris in the human eye is responsible for controlling the exposure of of light that reaches the retina. The movement of iris is controlled by a muscles called sphincter muscle and dilator muscle. sphincter muscle constricts the pupil in bright light while dilator muscle expands the pupil.

2.  Sternum belongs to the axial skeleton, which is a long flat bone located in the central part of the chest. Axial skeleton consists of 80 bones and strenum is one of them. Axial skeleton functions to provide protection to vital organs such as brain, heart and lungs. Strenum functions to protect heart.

2. If a nucleus has 8 chromosomes, how many chromosomes will be present after Telophase I? 3. What advantage does crossing-over have in reproduction? 6. Compare and contrast spermatogenesis and oogenesis (where do they occur, how many gametes are formed, when do they develop, etc):

Answers

Answer:

Telophase I: 8 chromosomes composed of two sister chromatids  

Crossing over refers to the mechanism of recombination, this process being characterized in that increases the genetic variation

Spermatogenesis refers to the mechanism of formation of male gametes (sperms), while oogenesis is the process of formation of female gametes (oocytes)

Explanation:

Telophase I is the final step in meiosis I. During telophase I, the nuclear membrane is formed around the decondensed set of chromosomes and the nucleoli reappear.  

Spermatogenesis refers to the process by which haploid male germ cells (sperms) are generated within the seminiferous tubules in the testis, while oogenesis refers to the process of differentiation of the female egg cells (ova or oocytes) in the ovaries. Spermatogenesis is known to produce small and motile spermatic cells, while oogenesis is known to produce bigger non-motile oocytes.

How do plants get the nitrogen they need?

A.
From bacteria living in their roots

B.
From the air

C.
Directly from the soil

D.
Through photosynthesis

Answers

the answer is C. because active transport happens at roots
The answer is Directly from the soil

why the swimmer push the water backward​

Answers

to go propele themselves across the water

Answer:

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Explanation:

Swimmers push water backwards to propel forwards. In other words, swimmers push their hands and legs against water to move forward. This is stated in the third law of motion, for every action, there is an equal and opposite reaction.

Classify each nutrient as a macronutrient or as a micronutrient

Answers

Answer:

Macro: Phosphorous, Protein, Fat, Carbohydrates

Micro: Vitamin A, Sodium

Explanation:

Macronutrients are nutrients that are needed in large amounts. Micronutrients are needed in smaller amounts.

Through which vascular tissue do water and nutrients get transported to reach the leaves during transpiration? cuticle parenchyma xylem phloem

Answers

Answer:

Xylem is one of the two types of transport tissue in vascular plants, phloem being the other. The basic function of xylem is to transport water from roots to stems and leaves, but it also transports nutrients.

Explanation:

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Answer:

C. xylem

Explanation:

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a strand of dna contains the base sequence AGTT . What is the sequence of the complimentary strand of DNA

Answers

Answer:

The sequence is TCAA

Explanation:

Deoxyribonucleic acid (DNA) is a molecule composed of two complementary chains of nucleotides. The nucleotides are in turn composed of one nitrogenous base, one deoxyribose sugar, and one phosphate group. The four different types of nitrogen bases found in DNA are thymine (substituted by Uracil during RNA transcription), adenine, cytosine and guanine. These bases bind by complementary base pairing to form an antiparallel double-strand. The complementary base pairing rule states that thymine forms two hydrogen bonds with adenine, while cytosine forms three hydrogen bonds with guanine.

Answer:

TCAA

Explanation:

A matches to T and G matches to C.

(5 points)
The organ responsible for filtering up to 95% of th
maintaining body pH is the
pancreas.
urethra.
kidney.
A pancreas
B urethra
C kidney
D gall bladder

Answers

Answer:

C. Kidney

Explanation:

The kidney is a vital organ in the human body. It is bean shaped and helps in the filtration of toxic substances and wastes from the body fluids which is then reabsorbed into the bloodstream.

The kidney also controls the level of water, pH and sodium levels. It however controls the blood pH by excretion of hydrogen ions and forming bicarbonate which ensures the blood pH is within its optimal and normal range.

Answer:

kidneys

Explanation:

The kidneys are the body's filtration system, and are responsible for filtering out the fluids in the body. The biproduct of this is called urine and is removed from the body

Knowing the epidemiology and causative agent of Legionaries disease what questions would you ask of the victims or of their surviving relatives?

Answers

Answer:

Answered below

Explanation:

Legionnaires disease is a type of lung inflammatory disease or pneumonia, which is caused by infection from the bacteria Legionella bacterium.

Legionella bacterium can be spread by inhalation from water or soil. People with weakened immune systems are most susceptible as are smokers. Untreated legionnaires disease can progress to a fatality.

Some of the questions to ask of a victim or of surviving relatives include;

1) Any visits to swimming pools?

2) Source of drinking water?

3)Any visits to fountains?

4) Contacts with victims and their personal belongings?

5)Is any relative or victim immunocompromised? Do they have diseases that might lower their immune response?

6) Are they smokers?

Angiosperms (flowering plants) and vertebrates obtain nutrients from their environment in different ways. a. Discuss the type of nutrition and the nutritional requirements of angiosperms and vertebrates. b. Describe 2 structural adaptations in angiosperms for obtaining nutrients from the environment. Relate structure to function. c. Interdependence in nature is evident in symbiosis. Explain two symbiotic relationships that aid in nutrient uptake, using examples from angiosperms and/or vertebrates. (Both examples may be angiosperms, both may be vertebrates, or one may be from each group.)

Answers

Answer:

a. Angiosperm are plants (autotrophs) that use photosynthesis to produce their own food, while vertebrates are animals (heterotrophs) that obtain their food from other organisms. Vertebrates include fish, amphibians, reptiles, mammals and birds.

b. Adaptations in angiosperms include two reproductive structures 1- fruits (ovaries), whose main function is to disperse the seeds and 2- flowers that ensure pollination and protect the embryo sac during its formation.

c . Examples:

1- The mycorrhizal symbiosis between plant roots and symbiotic fungi plays a key role in nutrient uptake from the soil. Arbuscular mycorrhizal is a type of symbiosis where a fungus of the phylum Glomeromycota penetrates the cortical root cells of vascular plants to produce specialized structures named arbuscules.

2- Microflora in the vertebrate digestive tract. The microorganisms that live in the digestive tract of vertebrate species are beneficial for the absorption of nutrients. For example, in humans, Saccharomyces boulardii has shown to have a probiotic effect by increasing intestinal homeostasis, thereby enhancing nutrient uptake.

whats 20x20? thank you

Answers

Answer:

400 yayyyyyy

Explanation:

yvhjghkkkkhhh

Answer:400

Explanation:

20

x20

_____

00

+400

--------

400

Why were the advent of metagenomics, and the application of molecular techniques such as single-cell and next-generation sequencing, important for defining a microbial relationship

Answers

Answer:

Based on novel approaches, it has been seen that symbiotic associations prevails between a host and one to thousands of more microbes, which challenges the previous meaning of symbiotic association as one that takes place between the host and a single microbe. It was considered previously that symbiotic association’s takes place only between the pair of single microbe and host.  

However, newer approaches has revealed that such kind of association can take place between one microbe and many other hosts. A symbiotic association can take place between microbe-microbe, plants-plants, microbe-plants, and others. However, symbiotic association has never been found to take place between a bacteria and animal host. The symbiotic associations are always not parasitic.  

Why are the polychaete worms living near the underwater volcanic vent called Pompeii worms?

Answers

Because of the volcanic eruption of pompeii

The polychaete worms living near the underwater volcanic vent are called Pompeii worms because they look like the ancient Roman city of Pompeii and because they are the most heat-tolerant animals on Earth.

What is the significance of polycheata?

Polychaetes are found in many parts of the world and play many important roles in the environment, such as their importance in marine and freshwater ecosystems. They also have a rich fossil record, which helps scientists study evolution, polychaetes are commonly used as model organisms in biological research, and polychaetes are studied to clean up contaminated environments through bioremediation.

Hence, the polychaete worms living near the underwater volcanic vent are called Pompeii worms because they look like the ancient Roman city of Pompeii and because they are the most heat-tolerant animals on Earth.

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Hemoglobin is the blood protein responsible for the transport of oxygen. Carbon monoxide disturbs oxygen transport by

Answers

Answer:

Answered below

Explanation:

Hemoglobin is found in the red blood cells and is responsible for the transportation of oxygen from the lungs to the various body cells.

Oxygen binds to hemoglobin because it has a high affinity for it. This aids its transportation. When it gets to the cells it unbinds and get transported into the cell for use in energy production.

Carbon monoxide is an odourless, dangerous gas which has more affinity for hemoglobin than oxygen. Therefore when carbon monoxide is inhaled, it quickly binds to hemoglobin, thereby displacing oxygen. It binds to hemoglobin for a longer period and due to this, the body does not get any oxygen and cells begin to die.

Symptoms of carbon monoxide poisoning include dizziness, headaches, fatigue and coma.

Which of the following techniques involves hybridizing a cDNA sample to a chip containing thousands of single-stranded DNA sequences, allowing one to study the expression of thousands of genes simultaneously?
A) PCR
B) Southern blot
C) FISH
D) Agarose gel electrophoresis
E) DNA microarray

Answers

Answer: Option E.

DNA micro array.

Explanation:

DNA microarray is a technique that involves hybridizing cDNA into a chip that contains thousands of single stranded DNA sequences. This technique enables one to study expression of thousands of genes at the same time.looks for extra (duplicated) or missing (deleted) chromosomal segments, sometimes called copy number variants (CNVs).

DNA microarrays are microscope slides which are printed with thousands of tiny spots in specific different positions, and each of the spots contain a known DNA sequence or gene.

primary use of DNA microarrays is transcriptional profiling.

The basic principle behind the DNA microarray is “ nucleic acid hybridization.

Fill in the blanks.
1. The phylum contains most common animals you are familiar with su
as eagles, dogs, foxes, and frogs.
2. While the earth is home to many members of this phylum, earthworms are
a member of
3. The most successful phylum on earth is which contains insects,
spiders, and crustaceans.
4. An animal that molts it exoskeleton is called a(n).
5. are cells that have the potential to become any type of cell; they hav
not yet received their genetic programming.
6. All members of the animal kingdom are multicellular, heterotrophic and
7. A starfish is a bilaterian deuterostome with symmetrical adults.
8. Complete the chart by describing what kinds of organs or tissues each type
germ layer becomes:
Moi

Answers

Answer:

1. chordata

2. nematoda

3. arthropoda

4. ecdysozoan

5. stem Cells

6. eukaryotic

7. radically

Chart answer:

Endoderm = Lungs, liver, pancreas, lining of gut organs

Mesoderm = Bone, muscle, heart, circulatory system

Ectoderm - Brain, nervous system, skin

Explanation:

Answer:

1. chordata

2. nematoda

3. arthropoda

4. ecdysozoan

5. stem Cells

6. eukaryotic

7. radially

8. Endoderm… Mesoderm becomes… Ectoderm becomes…

Lungs, liver, pancreas, lining of gut organs

Bone, muscle, heart, circulatory system

Brain, nervous system, skin

Explanation:

Other Questions
Help PLZ!!! Which statement accurately represents the arrangement of electrons in Bohrs atomic model? -Electrons move around the nucleus in fixed orbits of equal levels of energy. -Electrons move around the nucleus in fixed orbits of increasing levels of energy. -Electrons move randomly in the relatively large space surrounding the nucleus. -Electrons vibrate in fixed locations around the nucleus. The legal document identifying the rights and obligations of both the bondholders and the issuer is called the bond ______. This document describes the number of bonds authorized, their par value, and the contract interest rate. agreement document indenture article Grace starts with 100 milligrams of a radioactive substance. The amount of the substance decreases by 14 each week for a number of weeks, w. She writes the expression 100(14)w to find the amount of radioactive substance remaining after w weeks. Ryan starts with 1 milligram of a radioactive substance. The amount of the substance decreases by 40% each week for a number of weeks, w. He writes the expression (1 0.4)w to find the amount of radioactive substance remaining after w weeks. Use the drop-down menus to explain what each part of Graces and Ryans expressions mean. DeLong Corporation was organized on January 1, 2017. It is authorized to issue 14,500 shares of 8%, $100 par value preferred stock, and 450,000 shares of no-par common stock with a stated value of $3 per share. The following stock transactions were completed during the first year.Jan. 10 Issued 84,500 shares of common stock for cash at $6 per share.Mar. 1 Issued 5,150 shares of preferred stock for cash at $110 per share.Apr. 1 Issued 23,500 shares of common stock for land. The asking price of the land was $91,000. The fair value of the land was $81,500.May 1 Issued 84,000 shares of common stock for cash at $5.00 per share.Aug. 1 Issued 10,000 shares of common stock to attorneys in payment of their bill of $39,500 for services performed in helping the company organize.Sept.1 Issued 11,500 shares of common stock for cash at $7 per share.Nov. 1 Issued 2,000 shares of preferred stock for cash at $111 per share.Journalize the transactions. The mean one-way commute to work in Chowchilla is 7 minutes. The standard deviation is 2.4 minutes, and the population is normally distributed. What is the probability of randomly selecting one commute time and finding that: a). P (x < 2 mins) _____________________________ b). P (2 < x < 11 mins) _____________________________ c). P (x < 11 mins) ________________________________ d). P (2 < x < 5 mins) _______________________________ e). P (x > 5 mins) Identify how many distinct classes there were in Rome What is the image of the point by (-5,3) under a 270 rotation about the point (-7,-3) An inverse variation includes the point (4,17). Which point would also belong in this inverse variation? what is transmission of heat? Graph a line that contains the point (-7,-4)and has a slope of - 2/3 what is your understanding about politics?? n a group discussion about Frida Kahlo, Selma asks whether it is fair to say that Frida Kahlo is one of the most important female painters of the twentieth century. By asking this question, she has become the leader of the discussion. has provided her personal point of view. has become the moderator of the discussion. has provided an important fact about the topic. The graph of h(x) is a translation of f (x) = RootIndex 3 StartRoot x EndRoot. On a coordinate plane, a cube root function goes through (negative 3, negative 1), has an inflection point at (negative 2, 0), and goes through (negative 1, 1). Which equation represents h(x)? Question 4. In the graph, lines f and g intersect at P(6,6). What is the area, in square units, of the shaded region? * E. 15 F. 21 G. 27 H. 30 What is the allele frequency? Steve paid $3.29 for a pizza. He now has $35.86. With how much money did he start? Which of the phrases below best describes the environmental issue depictedin the following illustration?A. El efecto invernaderoB. La capa de ozonoC. El medio ambienteaD. La contaminacin A total of $10,000 is invested in two mutual funds. The first account yields 5% and the second account yields 6%. How much was invested in each account if the total interest earned in a year is $575? Suppose a firm in a competitive market earned $1,000 in total revenue and had a marginal revenue of $10 for the last unit produced and sold. What is the average revenue per unit, and how many units were sold? which one of the following electronic configuration represents the element that forms simple ion with a charge of-3? (a)1s^2,2s^2,2p^6,3s^2,3p^1 (b)1s^2,2s^2,2p^6,3s^2,3p^3 (c)1s^2,2s^2,2p^6,3s^2,3p^6,3d^1,4s^2 (d)1s^2,2s^2,2p^6,3s^2,3p^6