In creating recombinant DNA, some restriction enzymes produce ______ ends in both the source DNA and plasmid DNA, and these ends can stick to each other by ______.

Answers

Answer 1

Answer: Single-stranded; complementary base pairing

Explanation:

Answer 2

Restriction enzymes are essential components of recombinant DNA technology because they can be extracted from bacterial cells and utilized in the lab to modify DNA fragments, including those that contain genes (genetic engineering).

What recombinant DNA produce by restriction enzymes?

An isolated bacterial protein called a restriction enzyme breaks DNA at specific sequence-specific locations to produce DNA fragments with a known sequence at each end.

For several laboratory techniques, such as genetic engineering and recombinant DNA technologies, restriction enzymes are essential.

Therefore, In creating recombinant DNA, some restriction enzymes produce Single-stranded  ends in both the source DNA and plasmid DNA, and these ends can stick to each other by complementary base pairing.

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Related Questions

How many seconds would it take DNA polymerase to replicate one set of human chromosomes (6,000,000,000 bytes long)

Answers

The typical human chromosome has about 150 million base pairs that the cell replicates at the rate of 50 pairs per second. At that speed of DNA replication, it would take the cell over a month to copy a chromosome.

The typical human chromosome has about 150 million base pairs that the cell replicates at the rate of 50 pairs per second.

What is chromosome ?

A chromosome is a thread-like structure present in the nucleus of cells such as plant, animal and human cells and it is made of a molecule of DNA (Deoxyribonucleic acid) and histone proteins.

It is composed of three components, DNA, proteins and RNA,  amount of the DNA content ranges from 30 to 40 %, the amount of proteins from 50 to 65%, and small amount of RNA from 1 to 10%.

Two types of protein present in DNA such as histone and non-histones proteins, histone constitute large amount (90% of total protein), whereas non-histone is present in small amount (around 10% of total protein).

The histone protein is basic, whereas, the DNA is acidic, so it can be neutralized; DNA in chromosome  is present in two forms such as Euchromatin and Heterochromatin.

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Which of these is not an example your body maintaining homeostasis?

A-When exercising, your body releases more energy to your muscles.
B-On a hot summer day, you produce sweat.
C-When you get a virus, your body systems react to fight it.
D-When you see a piece of cake, your mouth waters.

Answers

Answer:

D. When you see a piece of cake, your mouth waters.

That’s between A & B because maintaining homeostasis is dealing with temperature and how your body reacts to it so I’ll most likely say B because that’s your body reacting on a hot summer day which is homeostasis so you sweat because you hot and also I’m also talking about maintaining homeostasis in school!

Which molecule binds NMDA receptors and prevents the flow of ions at resting and hyperpolarized membrane potentials

Answers

Answer:

it is in the cell

Explanation:

Aupporting cells of the nervous system are collectively called:

Answers

Supporting cells of the nervous system are collectively called neuroglia.

Answer:

They are called neuroglia or glial cells.

gene expression is determined by several factors, including

Answers

Hi! Thanks for asking your question here on Brainly!

Answer:

Chemicals in the body of a living organism is one of the many factors determined in gene expression.

Explanation:

The presence of drugs and/or chemicals in a living organism's environment, this can also influence gene expression in the organism.

Hope this helps :D Happy Holidays!

Expressivity can be impacted by a number of elements, such as genetic make-up, exposure to dangerous chemical substances, other environmental effects, and age.

What are the factors that affect gene expression?

The level of transcription is where the majority of control over gene expression is exerted, and this is mostly due to the fact that proteins bind to certain locations on DNA.

It is possible for environmental elements such as nutrition, temperature, oxygen levels, humidity, light cycles, and the presence of mutagens to have an effect on which of an animal's genes are expressed, which in turn has an effect on the animal's phenotype.

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Along one side of a DNA molecule, the nucleotides are connected to each other by ________ bounds between _______

Answers

Answer:

Nucleotides are joined together by covalent bonds between the phosphate group of one nucleotide and the third carbon atom of the pentose sugar in the next nucleotide.

Explanation:

Along one side of a DNA molecule, the nucleotides are connected to each other by covalent bonds between the phosphate group pf one nucleotide and the third carbon atom of the pentose sugar (deoxyribose sugar) in the next nucleotide.

How nucleotides are arranged in DNA?

Two nucleotides are attached via a phosphodiester bond to form dinucleotide. Another  nucleotides are added in same manner to form polynucleotide chain.

In this polynucleotide chain, the nucleotides are connected to each other by covalent bonds between phosphate group of one nucleotide and third carbon atom of deoxyribose sugar and forms a phosphate backbone of DNA.

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Name three components of the cell membrane and explain how each contributes the semipermeable nature of the membrane.

Answers

The principal components of the plasma membrane are lipids ( phospholipids and cholesterol), proteins, and carbohydrates. The plasma membrane protects intracellular components from the extracellular environment. The plasma membrane mediates cellular processes by regulating the materials that enter and exit the cell.

when do alleles for two different genes—located in two different chromosomes—segregate?

Answers

Meiosis is the answer

___________1. Variety of species and abundance of species
___________2. Refers to the heterogeneity present in the world or a habitat,
ranging from macromolecules within the cells to biomes
___________3. Plant or animal that exist in small numbers
___________4. It is the loss of species
___________5. Species in danger of extinction
___________6. Number of different species present in an ecosystem
___________7. Relative abundance of individuals of each of those species
___________8. Refers to the role of that a species play in its ecosystem
___________9. They serve as biological smoke alarms
__________10. The world’s largest coral reef
__________11. Also known as the four major causes of loss of diversity
__________12. The process during which a large expanse of habitat is
transformed into a number of smaller patches of smaller
total area
__________13. Species that are introduced accidentally or intentionally
__________14. Also called overharvesting
__________15. They have broad niches

Answers

post it again and give figure pls

look at the picture below. what word describes what is happening to the nutrients and water in the biome?

Answers

Given what we know about the water cycle we can confirm that the process that happening in the biome is the process of condensation

Although the water cycle can be divided and subdivided into various processes, we can simplify it into four simple steps:

EvaporationCondensationPrecipitation Collection

The one saw in the biome and one of the most essential parts of the cycle is condensation. It is the collection of water molecules into a single group that, when dense enough, fall back onto the earth as rainfall. In nature these take the form of clouds, meanwhile, in the biome, they appear as water on the sides of the container.

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Answer:

recyled

Explanation:

What type or piece of material culture do you think gives the most information about your society? Why?

Answers

material culture, tools, weapons, utensils, machines, ornaments, art, buildings, monuments, written records, religious images, clothing, and any other ponderable objects produced or used by humans. If all the human beings in the world ceased to exist, nonmaterial aspects of culture would cease to exist along with them.

HOPE THIS HELPS!!!!

what is the electron transport chain​

Answers

Answer:

The electron transport chain is a cluster of proteins that transfer electrons through a membrane within mitochondria to form a gradient of protons that drives the creation of adenosine triphosphate (ATP).

Explanation:

Brainliest Please!

Define negative feedback in biology

Answers

Answer:

A negative feedback loop occurs in biology when the product of a reaction leads to a decrease in that reaction. In this way, a negative feedback loop brings a system closer to a target of stability or homeostasis

Explanation:

Negative feedback occurs when some function of the output of a system, process, or mechanism is fed back in a manner that tends to reduce the fluctuations in the output, whether caused by changes in the input or by other disturbances

describe the negative impacts to an ecosystem of large-scale monocultures of crop
plants and of intensive livestock production

Answers

Answer:

CHECK THIS LINK YOUR QUESTION WILL BE ANSWERED.

https://www.goconqr.com/mindmap/4978927/negative-impacts-on-an-ecosystem-of-large-scale-monocultures-of-crop-plants

Explanation:

what is true of a fossil that is classified as a transitional form?

Answers

Given our understanding of the situation, we can confirm that a fossil classified as a transitional form, will show characteristics both of its ancestors and of the descendants that derived from it.

Transitional fossils are just that, fossilized remains of a transitional phase of evolution. What this means is that as species evolve, there will always be a point in time where a species is at a halfway point in between the species from which it comes, and the species that it will become.

These fossils, due to being a halfway point, will have characteristics that belong to their ancestors, but also characteristics that belong to their descendants, which is only logical considering that evolution is a process that spans many generations, not something instant.

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What is a muscle group that performs opposite functions called?

Answers

Answer:

b. Antagonistic muscles

Explanation: is correct

PLEASE HELP ME IM TIMED!

Answers

Answer:

Prokaryotic and anaerobic

Hope this helped

A student is conducting an experiment with biochemical reactions and notices that at high temperatures, the reaction rate decreases significantly.

Which statement best explains the decrease in reaction rate?


The bonds of the substrate were breaking.

The enzyme bonded with the substrate.

The enzyme denatured and changed shape.

The activation energy was increasing.

Answers

Answer:

The enzyme denatured and changed shape

Explanation:

Enzymes are proteins, which can only function properly within a certain range of temperatures- when things get too hot, proteins undergo a structural change and are considered to be 'denatured'. This means that enzymes will not be able to function properly under those high temperatures, because the substrate bonding site would no longer be the right shape for said substrate.

The statement best explains the decrease in reaction rate is the enzyme denatured and changed shape. Thus option 3 is correct.

What are enzymes?

Enzymes are defined as a material that functions as a catalyst in living things, controlling the pace at which chemical processes take place without changing the substance itself.

There are six types of enzymes.

HydrolysesOxidoreductaseLyasesTransferaseLigasesIsomerase

The functions of enzymes are:

In human bodies, enzymes facilitate metabolic reactions. From breathing to digestion, they support.Health issues might result from having a too amount or too little of a certain enzyme. To help their bodies function as they should, certain persons with chronic diseases may need to take enzyme supplements.

Thus, the statement best explains the decrease in reaction rate is the enzyme denatured and changed shape. Thus option 3 is correct.

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Which of the following statements is TRUE?

If you have minor symptoms of PID, you do not need to be treated.
PID always causes severe pain.
*PID can result in having problems getting pregnant later in life.
PID occurs in males.

Answers

Answer:

c. PID can result in having problems getting pregnant later in life.

Explanation: is correct. post protected

Give an example in which you control temperature to influence a chemical change.

Answers

Answer:

100* farenhights

Explanation:

Which of the following is true of gravity?

1 it is a pulling force

2 it holds objects in orbit

3 it is a bending of space

4 it can alter the path of light

5 all of the above

Answers

Answer:

5. All of the above

Explanation:

Hope this helps!

throughout the ___________, a healthy dietary pattern can help minimize diet-related chronic disease risk.

Answers

Answer:

pregancy

Explanation:

Throughout the life, a healthy dietary pattern can help minimize diet-related chronic disease risk.

What are chronic diseases?

Chronic diseases are those who required long term treatments.

Examples are cancer, heart diseases, diabetes, or any disability.

These diseases need treatments in phases or sometimes the treatment is lifetime.

To avoid chronic diseases, we should follow a healthy lifestyle.

Thus, the fill in the blank is life.

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What is the term describing the process that occurs when a species evolves into a new species without a physical barrier separating populations?

Answers

Answer:

Sympatric speciation should be correct.

Explanation:

Hope this helps.

how do you collect maple leaves in animal crossing

Answers

Answer:

Explanation:

Similarly to how Cherry Blossom Petals work during the Spring, you can get Maple Leaves by searching for a large orange leaf falling from the sky on your island during this limited period in the Autumn. It's random when and where these leaves will fall on your island, so always be on the look out for one to appear!

The Mississippi river carries sediments into the Gulf of Mexico. What do you think will happen to the sediments after a few million years?.

Answers

They build up and create an underwater barrier or island until they reach the surface

Answer:

Explanation:

The Mississippi River carries tons of tiny rock fragments called sediments into the Gulf of Mexico. What do you think will happen to these sediments after a few million years? Gizmo Warm-up Over millions of years, rocks are broken down and transformed into other rocks. The Mississippi river carries sediments into the Gulf of Mexico. What do you think will happen to the sediments after. The Mississippi river carries sediments into the Gulf of Mexico. What do you think will happen to the sediments after a few million years?

(compound microscope)
1. Put a table lamp in front of the?
2. Put a low-power (, e.g., x5) ________into
the microscope.
3. Rotate a low-power (e.g., x4)
______________into position.
4. Look through the eyepiece. Adjust the __________________ until you see a bright circle of light.
5. Place a slide on the _________, and fix it with the __________. The ___________ on the slide should be right above the hole of the stage.
6. Look at the microscope from the side. Turn the ______
carefully to lower the objective until it is very close to the ________.
7. Look through the eyepiece. Turn the coarse
adjustment knob to raise the objective slowly until
you can see an __________ of the specimen.
7. Turn the ________ to get a sharper image. The image is now in focus.


Choices: microscope, eyepiece, objective, coarse adjustment knob, slide, specimen,
mirror, stage, clips, fine adjustment knob, image

Answers

In this exercise it is necessary to fill the gaps with the correct given word. So, the right order of words will be:

1. microscope

2. eyepiece

3. objective

4. mirror

5. stage

6. clips

7. specimen

8. coarse adjustment knob

9. slide

10. image

11. fine adjustment knob.

So, after filling all the gaps, the text and, consequently, the final answer will be:

1. Put a table lamp in front of the microscope.

2. Put a low-power (, e.g., x5) eyepiece into  the microscope.

3. Rotate a low-power (e.g., x4)  objective into position.

4. Look through the eyepiece. Adjust the mirror until you see a bright circle of light.

5. Place a slide on the stage, and fix it with the clips. The specimen on the slide should be right above the hole of the stage.

6. Look at the microscope from the side. Turn the coarse adjustment knob

carefully to lower the objective until it is very close to the slide.

7. Look through the eyepiece. Turn the coarse  adjustment knob to raise the objective slowly until  you can see an image of the specimen.

8. Turn the fine adjustment knob to get a sharper image. The image is now in focus.

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once the food is swallowed, it passes throught the what

Answers

Answer:

esophagus

Explanation:

Once food is swallowed, it passes through the esophagus into the stomach

what is the most dangerous animal in the world 2021

Answers

Answer:

mosquitos ...........

Match the vocabulary word with the proper definition.

Definitions

_____ 1. organism that has cells containing a nucleus and other organelles

_____ 2. an organelle inside eukaryotic cells where the DNA is located

_____ 3. cell without a nucleus

_____ 4. a structure within the cytoplasm of a cell that is enclosed within a membrane and performs a specific job

_____ 5. phospholipid bilayer that surrounds and encloses a cell

_____ 6. first person to use the word “cell”

_____ 7. tiny, non-living particles that may cause disease

_____ 8. the material inside the plasma membrane of a cell

_____ 9. cell that contains a nucleus and other organelles

_____ 10. organelle where proteins are made

_____ 11. discovered human blood cells

_____ 12. a single-celled organism that lacks a nucleus



Terms

a. Anton van Leeuwenhoek

b. cytoplasm

c. eukaryote

d. eukaryotic cell

e. nucleus

f. organelle

g. plasma membrane

h. prokaryote

i. prokaryotic cell

j. ribosome

k. Robert Hooke

l. virus

Answers

Answer:

1) Eukaryotic Cell

2)Nucleus

3)Prokaryotic cell

4)organelle

5)Plasma membrane

6)Robert Hooke

7)Virus

8)cytoplasm

9)Eukaryote

10)Ribosom

11)Anton van Leewenhoek

12)Prokaryote

How many rounds of mitosis produce 64 daughter cells?(1 point)

4


2

6


8

Answers

The correct answer is 6 rounds.

Hope this helps!

Answer:

6

Explanation:

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A skater is spinning with his arms outstretched. He has a 2 lb weight in each hand. In an attempt to change his angular velocity he lets go of both weights (by just opening his grip). Does he succeed in changing his angular velocity what is a limiting factor that keeps populations from growing The mean of 11 numbers is 49. Removing one of the numbers causes the mean to decrease to 45. What number was removed? Thomas is a member of the U.S. Navy. He did not report for scheduled duty in several weeks. He did not request time off from his supervisor.What type of law applies to this situation?A) CivilB) MilitaryC) JuvenileD) Common The diagram above shows the cell cycle. As you can see from the diagram, the cell spends most of the time in _______________________ during which it undergoes rapid growth and the DNA is replicated in order to prepare for Mitosis Match the equation to the type of solution. 3=3 A. One solution x=0. B. No solution 4=-2. C. Infinite many solutions QUESTION 1Fill the blank with the appropriate negative form of will .No, the technician________ install the air conditioner tomorrow. He will install it next week.QUESTION 2Fill the blank with the appropriate negative form of will .Fred ______ be here for the 4 th of July. He will be in Chicago the first week of July.QUESTION 3Fill the blank with the appropriate negative form of will .Marsha ______ shop in Macys. She says that it is too expensive.QUESTION 4Fill the blank with the appropriate negative form of will .The current economic situation______ last much longer. It will change in the next few months. please help me need help So is costume designer a majors in college that Ill be able to pick? please somebody help me just look at the picture At what values of x, does f(x) = 0?A. 2B. 8C. -4D. 0E. -1F. 4 In a group of 84 kids, three-sevenths bought their lunches.Of those who bought their lunches got a slice of pizza? Write your fraction in simplest form A product is marked down 15%; the sale price is $127. 46. What was the original price?. Work is shown to solve for the system of equations. Why does the answer for y indicate that the system has no real number solutions.O The solution does not solve for X first.O The values for Y are square roots of negative numbers.O The values for y have square roots.O The values for Y have a positive and a negative value. ________ is a meditative procedure that is especially useful in controlling discomfort related to cancer. how can we manage tour from Heathrow Airport to London? 1. The word 'apt' in "once the interviewer decides, 'Yes. I'm going to hire thisperson,' he relaxes. At that point, he's apt to shift into a more comfortableposition" (Paragraph 4) meansA. unlikelyB. likelyC. unableD. not ready soldiers fighting in the first world war were forced to dig trenches as protection from Which sentence uses the underlined yocabulary word INCORRECTLY? A. My furtive attempt to sneak out was foiled by the squeaking door. B. The sedate, focused environment of the classroom was almost unreal. C. We are all the predecessors of cave men. The American customer satisfaction index scores of McDonalds restaurants grew from 61 in 2002 to 71 in the year 2014. Find the average rate of change for the data. Show work.