in gel electrophoresis, a loading dye is usually added to the sample of dna to be loaded in the wells of the gel. why would this be important?

Answers

Answer 1

Answer:

Gel loading dye is typically made at 6X concentration (0.25% bromphenol blue, 0.25% xylene cyanol, 30% glycerol). Loading dye helps to track how far your DNA sample has traveled, and also allows the sample to sink into the gel. ... It is important to use the same running buffer as the one used to prepare the gel.


Related Questions

Explain the relationship between the food we eat and the energy we have. What other reasons do we need to consume food?

Answers

The food we eat converts into energy in our body so we can operate.

Food is important because no food for a certain amount of time can cause health problems to death. This is the same for water.

The most common starting pathway for the breakdown of sugars is ___________________. a. respiration b. fermentation c. glycolysis d. anabolism

Answers

Answer:

respiration

Explanation:

Which of the following food items would be the most energy efficient to eat?
A. Grilled chicken
B. Steak
C. Apple
D. Shark fin soup

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Answer:

Apple

Explanation:

The food item that would be the most energy efficient to eat is an Apple. The correct option is C.

What do you mean by energy-efficient?

The meaning of energy-efficient is the objects that have high energy but are low in calories. Fruits and vegetables have ample amounts of energy and are good sources of natural sugar. These items are good for health and provide energy at the same time.

The food items that are given are grilled chicken steak, and shark fin soup are all non-veg items, and contain a large amount of fat in them. But apple is a fruit, and it is low calorie and rich in fibers and energy.

Thus, the correct option is C. Apple regarding the food items that would be the most energy efficient to eat.

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What is the term that describes the loud, high-pitched sound made with each inhalation that occurs when there is respiratory obstruction in the larynx or trachea

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Stridor, its as described in the definition above, a high pitched wheezing sound caused from a blockage

the vestibular system relies on hair cells that are similar in structure and function to those found in the auditory system.

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Answer:

There are five vestibular receptor organs in the inner ear: the utricle, the saccule, and three semicircular canals.

Explanation:

What is the base sequence of the DNA gene that originally produced this mRNA codon?

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Answer:

A, TCA. Using the corresponding bases working backwards, A becomes T, G becomes C and U becomes A. Hope this helps

Explanation:

8. Name three things that form during the cell cycle.​

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Answer:

Hair, Blood Cells, and Skin

PLEASE PLEASE SOMEONE HELP PLEASE BRAINILY I ASSURE YOU

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Salt marshes, mangroves, and seagrass beds absorb large quantities of the greenhouse gas carbon dioxide from the atmosphere and store it, thus decreasing the effects of global warming. ... Carbon sequestration — the process of capturing carbon dioxide from the atmosphere, measured as a rate of carbon uptake per year

noaa

After CO2 is produced by tissues, in what form is most of that carbon transported to the lungs for removal from the body

Answers

as dissolved HCO3 :)

The organelles of eukaryotic cells interact with each other to maintain homeostasis within the cell. The nucleus, endoplasmic reticulum, Golgi apparatus, chloroplasts, and mitochondria all contribute to protein transport, packaging, export, and energy harnessing. According to the endosymbiotic theory, certain organelles are proposed to have evolved from free-living bacteria that were engulfed by a larger cell. These organelles have multiple membranes, their own circular DNA, and can replicate by binary fission. The origin of which organelles are likely explained using the endosymbiotic theory?

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Answer:

mitochondria and chloroplast

Explanation:

USA test prep

Answer:

mitochondria and chloroplast

Explanation:

I did it

1. Where does the energy of food originally come from?​

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The energy content of all food molecules can be traced back to the Sun. It is the process of photosynthesis that locks the Sun's energy into simple carbohydrates like glucose.

the condition of having a diploid chromosome complement in which one chromosome lacks its homologous partner.

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Monosomy: the condition of having a diploid chromosome complement in which one chromosome lacks its homologous partner.

A trisomy is a chromosomal disorder characterized by an additional chromosome, so the person has 47 instead of 46.

Nullisomic is a genetic condition involving the lack of both the normal chromosomal pairs for a species (2n-2).

So, the correct answer is 'Monosomy'.

describe the function of the denticulate ligaments

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Answer:

The function of the denticulate ligament is the stabilize the spinal cord in the vertebral canal.

What is one way that microorganisms can be beneficial to humans? Question 11 options: Microorganisms cause disease. Microorganisms decompose wood. Microorganisms decompose waste. Microorganisms decay tooth enamel.

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Microorganisms decompose waste.

Answer: I’m am pretty sure the answer is microorganisms decompose waste.

Explanation:

This is a short question if anyone would be able to help me please i really need the help!!!!

Answers

What is it i hope i can helps you:(

what does it mean when we say a gene is turned off?

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When a gene is turned off, It no longer provides the directions for making proteins. this means that the proteins needed to fulfill a particular job ( let’s say, tolerate lactose ) aren’t produced.

why is it necessary to have 6co2 entering the chloroplast

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the photosynthesis reaction must be balanced. six carbons are needed to make glucose, so six CO molecules are needed.

if you were to take 10 breaths/min and inhaled about 600 ml/breath, your minute ventilation rate would be

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your minute ventilation rate would be 6 L/min :)

Differentiate How
does telophase II differ from
telophase I?

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Answer: Telophase I is the ending phase of the first division of meiosis which would split the parent cell into two daughter cells while telophase II is the ending phase of the second division of meiosis which would split the two daughter cell into four daughter cells and resulting in the end of the process.

Give an example of display behavior exhibited by a territorial animal.

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Answer:

Agonistic (aggressive) displays usually occur near the borders of a territory. When a strange howler monkey approaches the territory of others, resident males set up a tremendous din, warning the intruder off. Many songbirds sit on highly visible perches while singing, providing both auditory and visual displays.


mot nucleoxom co cau truc gom

Answers

Sorry i didn’t understand
Sorry

The Middle East and Eurasia (primarily Russia) have about 70% of the world's
Select one:
Оа.
natural gas reserves.
on
O b. coal reserves.
O coil reserves.
O d. peat reserves.

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a. Natural gas reserves

The stomata are flattened stacks of sacs and the --(blank)-- is a sticky substance where complex organic products of photosynthesis are made.

A. stomata
B. stroma
C. grama

Answers

the answer is well stomata

Answer: C

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Describing Cause and Effect Select the question mark. Choose the cause that brings about the effect. option a: the chicks hide in tall grass. b: not much grass grows on the nesting grounds. c: the chicks parents bring him good. d: the gray chick hatches.​

Answers

Answer:

Cause: Not much grass grows on the nesting grounds

Effect: Some Arctic tern chicks have nowhere to hide

For the following DNA segment, please make the RNA segment that matches it.
ATT CGA AAG

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Answer: DNA sequence: C C A C T C T T A C T T

mRNA sequence: G G U-G A G-A A U- G A A

Amino acid: GLY-GLU-ASN-GLU

Letter symbol: GENE

Explanation:

Whats up

DNA sequences that control traits are called
a. genes
b. proteins
C. enzymes
d. deoxyribose sugar

Answers

gene

Explanation:

the gene of a human being carried all the traits of the person which can be transferred to an embryo when fertilization occurs

A. Genes

Genes carry traits from the person to the embryo which is then given to the baby.

Time differences are detected by specialized neurons called coincidence detectors, while intensity differences are detected by a phenomenon called ______.

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Answer:

Sound Shadow

Explanation:

how many sperm are produced in the testes each day?

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Answer:

300 million..........

The Chemical composition of the cell membrane

Answers

Answer:

cell membrane

Explanation:

Cell membranes are composed primarily of fatty-acid-based lipids and proteins. Membrane lipids are principally of two types, phospholipids and sterols (generally cholesterol).

If you see a bird flying toward you this information is most likely processed in the: a. avian lobe b. temporal lobe c. occipital lobe d. limbic system

Answers

Occipital lobe processes visuals so, C.

If you see a bird flying toward you this information is most likely processed in the occipital lobe.

What is occipital lobe?

Occipital lobe lies at the back of the brain and is responsible for the vision, colour and motion of an object. It also helps in memorizing the shape, structure or face of an object.

The occipital lobe is situated in the cerebrum part of the brain between the parietal and temporal bone.

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Have i done this right, why did i not get the third mark 7.Are you sure that boys are more _______ than girls? To measure the current through and the voltage across a resistor in a circuit, you should place the ammeter in _____ with the resistor and the voltmeter in _____ with the resistor. With respect to the neurotransmitters associated with psychological disorders, _____ is related to major depressive disorder, while _____ is related to schizophrenia. white blood cells that circulate in the bloodstream and are an important part of the bodys immune system are called ________. Which of these are characteristics of third-person limited point of view? Choose three answers. The readers access to information is not limited to any one character. The narration follows the thoughts and feelings of only one character. The readers access to information is limited to just one character. The narration follows the thoughts and feelings of all of the characters. The thoughts and feelings of secondary characters are revealed through their words and actions Who was the fascist dictator of Italy who formed an alliance with Hitler during WorldWar 11?Joseph StalinBenito MussoliniFranklin RooseveltNeville Chamberlain A population has a mean of 80 and a standard deviation of 6. A sample of 49 observations will be taken. What is the probability that the sample mean will be larger than 82 Item 7How do chloroplasts support cellular functions?A) They provide structure to the cell.B) They synthesize, sort, and package proteins.C) They convert glucose into usable energy.D) They convert energy from the sun into glucose. what is ecosystem?hi guyswill anybody be my good friend solve pls brainliest Please help me please 38 What is the slope of the line on the graph? akiko sells 5 small paintings and 5 large paintings .Each large painting cost $20 more than a small painting .she earns $300. what is the price of a small painting What arguments does Hamilton make in support of lifetime tenure? what reason would support a counterclaim what happened to indentured servants who were freed 4. Fix the following sentence. First, tell me what is missing, then make thenecessary changes:The children on the bus.5. What is tense? x + y = 8 Now let x = 7. What is y? .1/2 of David's money is2/3 of Henry's money.(a) Express David's money as a fraction of Henry's money