In real life, sequencing data is never as clean and clear as the sequencing data presented here. Typically, sequencing machines do not read eDNA sequences perfectly, occasionally mistaking a C for a T, for instance (though this is often <1% of the time). Additionally, there will even be some variability of the barcode among individuals of the same species! How can sequences without 100% identity to a reference still be useful for biodiversity assessment

Answers

Answer 1

Answer:

by using consensus sequences and complementing DNA barcoding data with other sources of information (e.g., morphological, and ecological data)  

Explanation:

DNA barcoding refers to the taxonomic method used to identify and classify species by comparing DNA sequences. Some of the most used barcoding genes include the 16 subunit (16S) of the ribosomal RNA (rRNA) gene in prokaryotes, the Cytochrome c Oxidase I (COI) gene in animals, the Internal Transcribed Spacer (ITS) sequence in fungi and the RuBisCO gene in plants. A good gene for DNA barcoding purposes must exhibit intraspecific variability, conserved regions in order to synthesize appropriate PCR primers, and the sequence need to be short in length (100 to 1000 bases). The main problem associated with this identification method is the confidence value between genetic sequences. For example, when COI sequences are identical, there is a 6 percent of chance that these sequences belong to different species. On the other hand, the percentage of intraspecific variability among DNA barcoding sequences is variable, even among closely related species. This problem, as well as bioinformatic errors related to sequence reads, can be partially overcome by aligning genetic sequences (including outgroups) in order to obtain consensus sequences. Moreover, taxonomists reasonably argue that DNA barcoding data should be complemented with morphological and ecological data in order to achieve accurate species identification.


Related Questions

What is a basic function of a cell

Answers

Answer:

The answer is below

Explanation:

They provide structure and support, facilitate growth through mitosis, allow passive and active transport, produce energy, create metabolic reactions and aid in reproduction.

Hope this helps:)

Which of the following is not a normal body response to heat?
Select one:
a. dilation of peripheral blood vessels
b. increased sweating
C. decreased rate of muscle activity
d. increased retention of blood in the core region
e. increased water loss

Answers

Answer:

c. decrease rate of muscle activity

What is the function of digestive enzymes ?
Answer in brief..I'll reward.​

Answers

➜ Digetive enzymes convert the large and insoluble food molecules into small water molecules in the process of digestion. For example, pancreas secretes enzymes like amylase, trypsin and lipase. The enzyme amylase breakdown the starch, trypsin breaks down the proteins and lipase breaks down emulsified fats into simple sugars, amino acids and fatty acids, respectively.

Question 2 of 10
Which of the following is a product of fossil fuel combustion that contributes
to the greenhouse effect?
A. N2
B. O,
C. CO2
D. H2O2
SUBMIT

Answers

C carbon dioxide (co2)

can someone help me right now

Answers

I choose a Temperate Forest in Eastern USA (Tennessee/North Carolina)

4 Biotic factors in a Forest
Plants such as trees and shrubs
Animals such as moose
Insects such as ants and mosquitoes
Fungi such as mushrooms

4 Abiotic factors in a Forest
Sun proving sunlight to all the plants
Rocks creating a rough terrain
Water flowing in rivers to provide plants and animals with essential electrolytes and minerals
Rich Soil providing essential nutrients for plant growth, which in turn provide a food source for herbivorous animals such as moose.

One interaction I see between biotic and abiotic factors is that the water flowing in rivers and streams inside my Temperate Forest is providing essential electrolytes and minerals such as Magnesium and Calcium to animals such as moose and deer drinking the water, or plants nearby the stream, who utilize the water in their process of cellular respiration to create glucose which feeds the rest of the plant and in turn will feed an herbivorous animals looking for fresh plants to eat.

g Classify the statements as describing deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). DNA RNA is a single molecule that can be over 10,000,000 nucleotides long is usually double stranded includes the base thymine can be translated into a protein is the genome for prokaryotic organisms is usually single stranded includes ribose sugar is a single molecule that can form a complex secondary structure

Answers

Answer:

DNA:

- is a single molecule that can be over 10,000,000 nucleotides long

- is usually double stranded  

- includes the base thymine

- is the genome for prokaryotic organisms

RNA:

- can be translated into a protein  

- is usually single stranded  

- includes ribose sugar  

- is a single molecule that can form a complex secondary structure

Explanation:

Deoxyribonucleic acid (DNA) is the hereditary genetic material of both prokaryotic and eukaryotic organisms, while RNA is the genetic material of many viruses. DNA is a double helix molecule composed of four types of nucleotides: a nitrogenous base (Adenine, Thymine, Cytosine and Guanine), a five-carbon sugar (i.e., deoxyribose), and a phosphate group. On the other hand, RNA is usually a single-stranded molecule whose nucleotides contain ribose sugars and Uracil bases replace Thymine bases. During translation, a type of RNA molecule referred to as messenger RNA (mRNA) is used as template to direct the synthesis of a polypeptide chain (protein). This RNA molecule can fold to form stable double-stranded RNA hairpins (i.e., secondary structures).

What are sedimentary rocks ?? USE IN YOUR OWN WORDS.

40 POINTS DONT SKIP !

Answers

Answer:

Rock that has formed through the deposition and solidification of sediment

Explanation:

Fungi are all eukaryotes that are not animals, plants or protists.
TRUE
FALSE
Check it

Answers

Answer:

true

Explanation:

this is it I think.:)

Question:
Soil is sometimes called a bridge between life and the non-living world. Explain what that means.
es )

Answers

Explanation:

The non living things in its environment is called a " matrix,"

A matrix is what supports a non living thing to reproduce, triple its existence & reproduce in mass quantities, like soil bacteria, and others..

If an animal has 21 chromosomes before replication, how many do they have after?

Answers

Answer:

Animals usually have 22 pairs of homolog chromosomes (autosomes) and as sex chromosomes 2 x chromosomes (in female), or 1 x and 1 y chromosome (in males), thus in total 46 chromosomes per body cell (see human karyogram; 2n = 46) and 92 chromatids per cell after replication.

Explanation:

42

Given the following sequence of DNA, write the correct sequence of complementary DNA bases.

TACCGATAA

Given the following sequence of DNA, write the correct sequence of complementary mRNA.

ACCGTACTG

Given the following sequence of DNA write the correct sequence of amino acids.

ATAGGCGTA

Answers

Answer:

1. ATGGCTATT

2. UGGCAUGAC

3. mRNA: UAU-CCG-CAU, amino acids: tyrosine(tyr)-proline(pro)-histidine(his)

Explanation:

For DNA, the complementary bases are T=A and C=G. This means every time you see one of these letters, the corresponding letter goes with it. For mRNA, the bases are T=A, A=U, C=G. It is the same except now, there should be no "T" in the mRNA sequence. With amino acids, you would first code for the mRNA sequence, every three letters being the amino acid sequence. To find the amino acid, you would look at a codon chart unless your teacher wants you to memorize them.  The first three letters of the amino acid are the abbreviation.

Question 7
5
Parents are exposed to hazardous chemicals. How might this affect their
future offspring?
The chemicals could make the parents sick before they have offspring,
The offspring could inherit damaged DNA from the parents.
The offspring could have red hair

Answers

Answer:

The offsprings could inherit damaged DNA from the parents.

Explanation:

The hazardous chemicals may cause mutations to the DNA.

How much energy will the cheetah get if it eats the gazelle?

Answers

Answer:

alot

Explanation:

what are the limitations of cell theory​

Answers

Answer:

The shortcomings or drawbacks of cell theory are: Viruses are considered as acellular entities or organisms that do not have cell machinery, yet they are taken into account as organisms in this cell theory. Matthias Schleiden and Theodor Schwann did not know the mechanism of the cell.

Explanation:


Increased carbon dioxide in the atmosphere leads to increased absorption of
Greenhouse Gases
Solar Radiation
Water Vapor
Volcanic ash

Answers

Answer:

Solar Radiation

Explanation:

Increased carbon dioxide in the atmosphere leads to increased absorption of Solar Radiation.

If a plant fall which way will the roots grow

Answers

in the direction of the gravitational pull

Answer: The roots grow whichever way gravity will push them.

how many degrees in a triangle ​

Answers

Answer:

The internal degrees in a triangle add up to 180

Explanation:

Answer:

Three are three angles in a triangle which all add up to exactly 180 degrees

Explanation:

Which of the three fundamental tissues have a secondary cell well? (Parenchyma, collenchyma and sclerenchyma)

Answers

The answer to your question is Sclerenchyma cells have thick lignified secondary walls. Answer: Sclerenchyma

what is xylem and phloem in science?​

Answers

Answer:

The xylem is one of the two types of tissue transported from the vascular plants, the other being the phloem.

Answer:

Xylem and phloem Plants have tissues to transport water, nutrients and minerals. Xylem transports water and mineral salts from the roots up to other parts of the plant, while phloem transports sucrose and amino acids between the leaves and other parts of the plant. Xylem and phloem in the center of the plant root

Explanation:

Hope this helps!!

name and explain the emergent properties of water?​

Answers

Answer:

Water has high specific heat, high heat of vaporization, and an evaporative cooling property that together causes it to tend to maintain a constant temperature. Water temperatures can change, of course, they just change more slowly than the temperatures of other substances.

Am I correct? Tell me

Answers

Answer:

Yep, at least from what I know.

Correct

Explanation:

Answer:

I believe you are correct.

Explanation:

Air masses form over regions where the air is stable for a long enough time. The air takes on the characteristics of the region. Air masses move when they are pushed by high level winds.

HELPPPPPPPPPPPPPPPPPPPP

Answers

Answer:

Grabbing a handful of mixed candy out of a bowl.

Explanation:

For all other options you are choosing specific items... such as a book from the library, or choosing items written on your grocery list, or choosing what to pack for lunch. Bycatch in fishing refers to catching extra fish, other than what you are selecting for. By grabbing a handful of mixed candy from a bowl you are not selecting for a specific 'fish'.

Plz help/will give brainlest!​

Answers

It’s B hope I have a nice day
I know one of the answers is the third choice

money supply and inappropriate government policies of economics fluctuations ​

Answers

Answer:

Both are responsible for the economics fluctuations.

Explanation:

Fluctuations occurs in the economic condition of a country due to the inappropriate polices of the government because polices of government has direct impact on the the growth of economy of a country whereas the money supply tells us the current status of the economy of a country. The money supply refers to the total value of money available in an economy at a specific time so we can conclude that both money supply and inappropriate government policies leads to the economics fluctuations of a country.

The process of fermentation takes place

Answers

Answer:

Fermentation is an anaerobic process in which energy can be released from glucose even though oxygen is not available.

Explanation:

Fermentation occurs in yeast cells, and a form of fermentation takes place in bacteria and in the muscle cells of animals.

d) During Transcription, a nucleotide has been deleted. Explain the effect this will have
on DNA as well as what happens in each step along the way to a protein.

Answers

Answer:

this mutation may change the open reading frame of the resulting RNA sequence and its final product, which is a protein in the case that this gene is used to synthesize a messenger RNA (mRNA) sequence

Explanation:

During the transcription, a region of DNA named 'gene' is used as template to produce an RNA molecule, typically a primary transcript of mRNA (pre-mRNA). Subsequently, this pre-mRNA suffers a process named RNA processing in order to generate a mature mRNA which is finally used to create a protein by a process called translation. If a deletion occurs during transcription, it may change the open reading frame (ORF) of the resulting mRNA when the mutation occurs in an exon of the protein-coding gene (i.e., occurs a frameshift mutation), while this deletion may not have any effect if it is localized within the introns which are removed during RNA processing. A frameshift mutation will change the amino acids that are added to the nascent polypeptide chain during translation.

Match the term to its description.


Match Term Definition

Elliptical galaxy A) Has a central core from which curved arms spiral
outward

Irregular galaxy B) Has a large flattened core

Lens galaxy C) Forms a perfect sphere or an ellipse and is
flattened to some degree

Spiral galaxy D) Does not have a distinct regular shape

Answers

Answer:

Hello!

Explanation:

☁️Match the term to its description.

⠀⠀⠀⠀⠀Match Term Definition

--------------------------------------------------------------------------------------------------------------------------

Elliptical galaxy A) Has a central core from which curved arms spiral outward

Irregular galaxy B) Has a large flattened core

Lens galaxy C) Forms a perfect sphere or an ellipse and is flattened to some degree

Spiral galaxy D) Does not have a distinct regular shape

--------------------------------------------------------------------------------------------------------------------------

correction

Elliptical galaxy A) It forms a perfect sphere or an ellipse and is

flattened to a certain extent

Irregular galaxy B) Does not have a distinctive regular shape

⭐Galaxy lens C) Has a large flattened core

⭐Spiral galaxy D) It has a central nucleus from which curved-armed spirals

Exterior

I hope I've helped :D

regards

atte:

Ángela

❤️

Answer:

yup

Explanation:

he's wrong

I did it and I got the question wrong.

all of it

Can small carbon compounds be formed into large carbon compounds?

Answers

Answer:

ya it can also be the largest compound but it needs alot if pressure

Carbon is the only element that can form so many different compounds because each carbon atom can form four chemical bonds to other atoms and because the carbon atom is just the right, small size to fit in comfortably as parts of very large molecules.

NEED HELP ASAP

Which of the following increase the speed of action potentials in the nervous system?

Answers

Answer:

Where are the answers to choose from?

In any case, the ranvier nodes and myelin increase the speed

A man with AB blood is married to a woman with AB blood. What blood types will their children have

Answers

Answer:

A AB and B all three are possible

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