in the gel electrophoresis lab, you determined the ________ of three different dna samples.

Answers

Answer 1

In the gel electrophoresis lab, you determined the genotype of three different DNA samples.

Gel electrophoresis is a process that allows you to separate fragments of DNA based on size. You place different size fragments of DNA into a gel, then apply current to the buffer surrounding the gel, the DNA will travel through the gel but move at different speeds based on the different fragment sizes. Larger fragments of DNA will move slower than smaller fragment sizes.

Let consider two different alleles:

the bigger allele -- Athe smaller allele -- a

The bigger allele happens to contain a restriction site that is allowed to be cleaved by a restriction enzyme while the smaller allele has a mutation in the restriction site that means that it will not be recognized and cleaved by that same restriction enzyme.

Let us remember that the person will have two copies of this gene and can be:

H0m0zygous dominant AAh0m0zygous recessive aaheterozygous Aa

An individual with h0m0zyg0us AA only have versions of the gene that will be cut by the restriction enzymes, each allele producing a small fragment of DNA and a medium-size fragment of DNA

An individual with the genotype aa only has the version of the gene that does not have the restriction site and therefore does not get cut by the restriction enzyme. The DNA sample from this individual will have a single larger fragment of DNA.

When you isolate DNA from heterozygous Aa, half of the DNA isolated has the allele with the restriction site that gets cut into two fragments while the other half will have the allele that does not have the restriction site and remains whole when a restriction enzyme is added.

Therefore, we can conclude that in the gel electrophoresis lab, you determined the genotype of three different DNA samples.

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Related Questions

19. Engineer It In some rivers and lakes, dams
are constructed to harvest energy by converting the
kinetic energy of moving water into electrical energy.
Water from behind the dam flows through a turbine
that transforms kinetic energy into mechanical
energy. The turbine turns a generator that converts
mechanical energy into electrical energy.
The water from behind the dam could flow to the
turbine either through an opening near the middle or
top of the dam or by flowing over the top of the dam.
Which type of water flow would be the best design for
sending a consistent flow of water past the turbines?
Explain your reasoning.

Answers

Answer:

A hydroelectric dam converts the potential energy stored in a water reservoir behind a dam to mechanical energy—mechanical energy is also known as kinetic energy. As the water flows down through the dam its kinetic energy is used to turn a turbine.

Explanation:

A three-paneled painting is called a __________. A. Diptych b. Triptych c. Polyptych.

Answers

Answer: B Triptych

Explanation: A three-paneled painting is called a Triptych.

Answer:

b.triptych

Explanation:

correct on ed

A cross in which only a single trait with two variations is considered is called a(n) cross or single factor cross.

Answers

Answer:

Monohybrid

Explanation:

which hormone increases acid secretion by the stomach?

Answers

Answer:

gastrin

Explanation:

During meal ingestion, the main hormone responsible for stimulating acid secretion is gastrin, which acts primarily by releasing histamine from enterochromaffin-like cells.

Does the waxy cutie affect where acertain pla y could be grown? Why?

Answers

Waxy skin - some leaves have a thick, waxy skin on their surface. This reduces water loss by transpiration . Water storage - some plants, known as succulents, store water in their stems, leaves, roots or even fruits.

The waxy covering on plant leaves, young stems, and fruit is called the "cuticle". ... The purpose of this covering is to help the plant retain water. In arid regions, that is very important. In wetter regions, the waxy coating may help prevent infection by disease organisms.

Plants that grow in arid zones of USDA zones 9 to 11 use waxy leaves to limit transpiration and retain water but also to protect leaves from the frigid temperatures sometimes found in the desert. Trees like the desert willow (Chilopsis linearis) have narrow, waxy leaves and striking purple flowers.

- BRAINLIEST answerer

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Answers

I also have the same problem

SOMEONE PLEASE HELP

What is a positive effect of the technological advance of going from a compass to a gps

Answers

Answer:

The positive effect of the application of a GPS over a compass is that one can get success in getting to his or her desired location. A GPS can inform about the exact location, and when it is combined with a map, it makes the person reach straight to where he or she desires to go

Explanation:

what does the word gastropoda mean​

Answers

Answer:

a large class of mollusks which includes snails, slugs, whelks, and all terrestrial kinds.

Explanation:

hope that helps :D!

after dogs, what is thought to be the first domesticated farm animal?

Answers

Answer:

sheep and goats

Explanation:

label the diagram below of the root cross section
Plzzzzz help ASAP

Answers

The labeling of the root cell is as follow:

The outer red part is the Epidermis. The inner white part is the Cortex. The innermost pink part is the Xylem.

Parts of a Root Epidermis - protects the tissues. Cortex - allows for transportation of materials. Xylem - allows for transportation of water.

In conclusion, the root is quite complex and has various parts to allow it fulfil its functions in keeping the plant alive.

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When muscle tissue is exercising under anaerobic conditions, the production of ______ is important because it ensures a continuous supply of NAD

Answers

Given what we know about the biological mechanisms behind anaerobic exercise, we can confirm that the production of lactate ensures a continuous supply of NAD.

In order for us to undergo any physical exercise, it is imperative that we are able to produce energy within our cells. The energy used by cells is in the form of a molecule by the name of ATP, which is produced primarily through a process known as Glycolysis.

This is relevant given that in order for glycolysis to happen, a key protein is required known as NAD+. Lactate is produced from pyruvate only under anaerobic conditions because these are conditions that lack oxygen which is the only other source of NAD+, therefore, pyruvate must be converted to lactic acid to make it possible to continue glycolysis and the production of ATP.

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A sieve is a container with a mesh or perforated bottom. What is the most common use for a sieve? A. to steam cook chemicals in the lab B. to store liquid substances C. to allow gases to escape from reactions D. to filter larger particles out of a mixture

Answers

The answer is D to filter please brainlyist

Answer: D

Explanation: Sieves are used to separate the solid smaller particles from a mixture. They are usually made of metal or fine plastic mesh, with openings between the wires that are sized according to the sizes of the desired particles in the mixture. Dried foods such as beans and rice are separated by using screens with openings of different sizes, small for whole grains, larger for cracked grains. Other kinds of products which require separation may be filtered through sieves, including sugar mixtures before they are packed or bottled.

The effect of pH on a certain enzyme is shown in the graph. At what pH would the enzyme be most effective?

Answers

Answer:

2

Explanation:

The enzyme will be the most effective between 8 and 10

The enzyme will show maximum activity in the range of pH between 8 and 10. This can be the enzyme's optimum pH as at this range it is found to be most effective. Thus, the correct option is 2.

What are enzymes?

Enzymes are the biocatalysts. They act as enzymes in the biochemical reactions by reducing the activation energy required by the reactants to form products. Enzymes remain unused in a reaction.

Enzyme activity depends upon different factors which include temperature, pressure, pH, etc. Enzymes show maximum activity at the optimum conditions of these environmental factors.

From the given graph, it can be interpreted that the enzyme shows maximum rate of action between the pH range of 8 and 10. Thus, this is the optimum range of pH.

Therefore, the correct option is 2.

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(LO 1.6) Insulin
is usually broken down during glycolysis.
is a hormone released when blood (glucose) is high.
is hydrophobic.
is released when you are fasting (not eating).
is a channel for glucose found in the inner mitochondrial membrane.

Answers

Answer:

is a hormone released when blood (glucose) is high

stem cells that not form embryos are embryonic stem cells​

Answers

Answer:

Fuels Data Center

Ethanol is a domestically produced alternative fuel most commonly made from corn. ... such as crop residues and wood—though this is not as common.

https://afdc.energy.gov › AFDC

Alternative Fuels and Advanced Vehicles

Ethanol. Ethanol is a widely used renewable fuel made from corn and other plant materials. It is blended with gasoline for use in vehicles.

People also ask

Which is a negative impact of the use of ethanol for energy?

What is the use of ethanol as an alternative fuel?தமிழில் தேடுங்கள்

எத்தனால் என்பது நமது கார்கள் மற்றும் டிரக்குகளை இயக்குவதற்கான மாற்று எரிபொருளுக்கு ஒரு எடுத்துக்காட்டு.

"hsjajajakqk structure and function?How are glucose and ethanol similar in structure and function?How are glucose and ethanol similar in structure and function?How are glucose and ethanol similar in structure and function?How are glucose and ethanol similar in structure and function?Ethanol fermentation, also called alcoholic fermentation, is a biological process which converts sugars such as glucose, fructose, and sucrose into cellular energy, producing ethanol and carbon dioxide as by-products.Ethanol fermentation, also called alcoholic fermentation, is a biological process which converts sugars such as glucose, fructose, and sucrose into cellular energy, producing ethanol and carbon dioxide as by-products.Ethanol fermentation, also called alcoholic fermentation, is a biological process which converts sugars such as glucose, fructose, and sucrose into cellular energy, producing ethanol and carbon dioxide as by-products.Ethanol fermentation, also called alcoholic fermentation, is a biological process which converts sugars such as glucose, fructose, and sucrose into cellular energy, producing ethanol and carbon dioxide as by-products.Ethanol is blended with gasoline at the fuel terminal to make E10, E15, or E85, ... Ethanol has the same chemical formula regardless of whether it is ...Ethanol is blended with gasoline at the fuel terminal to make E10, E15, or E85, ... Ethanol has the same chemical formula regardless of whether it is ...Ethanol is blended with gasoline at the fuel terminal to make E10, E15, or E85, ... Ethanol has the same chemical formula regardless of whether it is ...How are glucose and ethanol similar in structure and function? How do they differ?.How are glucose and ethanol similar in structure and function? How do they differ?.How are glucose and ethanol similar in structure and function? How do they differ?.How are glucose and ethanol similar in structure and function? How do they differ?.answer up thereanswer up thereanswer up thereanswer up thereanswer up thereanswer up thereanswer up thereanswer up thereanswer up thereanswer up thereLimiting factors fall into two broad categories: density-dependent factors and density-independent factors. ... Other density-independent factors include hurricanes, pollutants, and seasonal climate extremes. Density-dependent limiting factors tend to be biotic—having to do with living organisms.Limiting

Limitations of artificial classification

Answers

A major disadvantage of artificial classifications is that they have little predictive value; arbitrarily selected properties do not necessarily correlate well with each other. This generally means that they are of little use for purposes other than that for which they were designed.

Mountains can be formed when one plate is forced under another. The plate on top folds up like an accordion and forms mountains. The plate forced under usually melts. What event is most likely to occur after much of the plate forced under has melted?

A.a flood B.a tsunami C.a volcano eruption D.an avalanche

Answers

Answer:

C. Volcanic Eruption is the answer

One way that antibiotics can treat bacterial disease without harming human cells is by:

Answers

By protecting bacteria from synthesizing cell walls.

Which of the following choices is a component in the habitat of a wetlands ecosystem?A. Moss
B. Bacteria
C. Water
D. Blue Heron

Answers

Answer: C.-Water

Explanation: Water is the main component when it comes to wetlands.

Habitat is the environmental place where organisms like plant and animal lives. Water is the habitat component present in the wetland ecosystem. Thus, option C is correct.

What is an ecosystem?

An ecosystem is a biological community that lives together and has an interconnection with each other as well as their surroundings and other abiotic factors.

The ecosystem can be found in freshwater, marine, or terrestrial places. The wetland ecosystem is covered by water in all seasons and is seen in the form of swamps, marshes, etc.

The wetland is characterized by the presence of water that controls the erosion, water quality, abatement of the flood, etc.

Therefore, water is a component of the wetland ecosystem.

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11. Describe the potential energy at each letter in the image to the
right:
W)
X)
Y)
Z)

Answers

Answer:

THE ANSWER IS Z

Explanation:

Y=X X 14 FUFFN C CCN. J.

Will give brainliet no file plz

Answers

Answer: Hey Hi Where is the question??

Which population would most likely be able to persist over many generations, despite having limited genetic variability

Answers

Answer:

A population in a relatively stable and static environment

Explanation:

How is genomics related to genes and DNA?

Answers

Answer:

A gene consists of enough DNA to code for one protein, and a genome is simply the sum total of an organism's DNA.

Genomic DNA, or gDNA, is the chromosomal DNA of an organism, representing the bulk of its genetic material. ... In research, genomic DNA is useful tools in applications such as PCR, library construction, Southern blotting, hybridizations, SNP analysis, and molecular diagnostic assays.

Answer:

DNA

DNA is the molecule that is the hereditary material in all living cells.

Genes are made of DNA, and so is the genome itself. A gene consists of enough DNA to code for one protein, and a genome is simply the sum total of an organism's DNA.

DNA is long and skinny, capable of contorting like a circus performer when it winds into chromosomes. It's skinny as a whip and smart as one too, containing all the information necessary to build a living organism. In a very real sense, DNA is information.

WHAT IS DNA MADE OF?

DNA is a very large molecule, made up of smaller units called nucleotides that are strung together in a row, making a DNA molecule thousands of times longer than it is wide.

Each nucleotide has three parts: a sugar molecule, a phosphate molecule, and a structure called a nitrogenous base. The nitrogenous base is the part of the nucleotide that carries genetic information, so the words "nucleotide" and "base" are often used interchangeably. The bases found in DNA come in four varieties: adenine, cytosine, guanine, and thymine—often abbreviated as A, C, G, and T, the letters of the genetic alphabet.

How did people find out that DNA is the hereditary material?

DNA was largely ignored for decades after a German chemist, Friedrich Miescher, first isolated the white, slightly acidic substance from the nucleus of cells in 1869. No one knew what DNA's function was—in fact, some doubted that it had a function at all—so they pretty much left the stuff alone.

Very few people thought that DNA could be the hereditary material. Early studies of DNA suggested, erroneously, that the molecule was made up of the same sequence of four bases repeated over and over—ACGTACGTACGT… for example. No one could imagine how such a monotonously simple molecule could contain the information necessary to build a living organism.

But during the 1930s and 1940s, new experiments began to suggest that DNA might, in fact, be important. It turned out that different strains of bacteria can exchange DNA and that when they do certain traits, such as the ability to cause disease in humans, can be passed from one strain of bacteria to another. Scientists also learned that when a virus infects a cell it injects its DNA into the cell, which then produces many copies of the virus, suggesting that DNA contains instructions for building viruses. And they found that different species of organisms have different proportions of bases in their DNA—one species might have DNA that is 30 percent A, 20 percent C, 20 percent G, and 30 percent T, while another might have 20 percent A, 30 percent C, 30 percent G, and 20 percent T. People began to think that genetic information might be written in the differences between the DNA bases of different species.

What does DNA look like?

A DNA molecule is a double helix, a structure that looks much like a ladder twisted into a spiral. The sides of the ladder are made of alternating sugar and phosphate molecules, the sugar of one nucleotide linked to the phosphate of the next. DNA is often said to have a sugar and phosphate "backbone."

Each rung of the ladder is made of two nitrogenous bases linked together in the middle. The length of a DNA molecule is often measured in "base pairs," or bp—that is, the number of rungs in the ladder. Sometimes, this unit of measurement is shortened simply to "bases."The structure of DNA was worked out in 1953 by James D. Watson and Francis Crick, who worked together in the Cavendish laboratory in Cambridge, England. By the time they began their work in the early 1950s, it was clear that DNA is the hereditary material, and scientists were racing to find out more about the long-ignored molecule, picking apart the implications of each new detail. Everyone knew they couldn't really understand how DNA works until they understood how its nucleotide building blocks are put together.

(Im a Really fast Typer and Thinker)

the more energy that is present the slower the matter is able to move.
True or False

Answers

Answer: False.

Explanation:

Energy heats up molecules, making them move faster.

False. Guy above me has it right

What is the job of the enamel on your teeth?

Answers

Explanation:

Enamel helps protect your teeth from chewing, biting, grinding, crunching, and so on. Enamel also serves to sort of protect the teeth from potentially harmful temperatures and chemicals.

Members of the Native American Standing Rock Sioux tribe share history and bonds based on a common lineage of blood relatives. This form of relationship is also known as

Answers

Given what we know about the dynamics of distinct relationship types, we can confirm that the shared history and bonds based on a common lineage of blood relatives describe a relationship known as a kinship.

Kinship is the relationship being described in the question, it does not leave any room to further describe this word as its precise definition is that it is a bond based on common lineage and blood relationships.

The remaining options for the question have a common theme. These options are:

Household

Cohabitation

Village

What each of these have in common and the reason they do not describe the relationship being described in the question due to the fact that none of these options include a blood relation to the members involved. There are all situations of individuals living together, often with a common lineage, but do not specify a blood relationship.

This question was answered based on the complete question found online which states:

Members of the Native American Standing Rock Sioux tribe share history and bonds based on a common lineage of blood relatives. This form of relationship is also known as

a. cohabitation.

b. a household.

c. kinship.

d. a village.

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the right lung has ________ lobes, and the left lung has ________ lobes.

Answers

Answer:

3,2

Explanation:

Which type of animal have thick skin and long Eyelashes?​

Answers

Answer:

Camel

Explanation:

When dissolved carbon dioxide (CO2) combines with ocean water it forms a(n)

Answers

Answer:

Carbon dioxide (CO2) dissolves in the ocean and reacts with water to form carbonic acid—which in turn generates bicarbonate, carbonate, and hydrogen ions. Calcium and carbonate ions combine to form the solid calcium carbonate crystals in their shells and skeletons.

Explanation:

Part 3

What type of front is front 1? What types of weather conditions take place there?

U

Font Sixes

Characters used 0 / 15000

Submit

Answers

Answer:

Front 1 is a cold front.

Explanation:

At a cold front, cold air moves in, causing a drop in temperature.

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Which sentence with a compound subject joined by "or" has correct subject/verb agreement? (Use the Proximity Rule!) A. Either laptops OR watches is the best way to get on the internet. B. Either tablets OR phones is the best way to get on the internet. C. Either laptops OR computers are the best way to get on the internet. D. Either phones OR a computer are the best way to get on the internet. The molecule is illustrated in figure ________.A) is a saturated fatty acid B) stores genetic informationC) will be liquid at room temperature D) is a carbohydrate Can you use volume alone to predict whether an object will sink or float? Explain your answer Help with this and explain please Hi! I'm looking for some proficient actually (accurate) responses. depending on the responses ill probably give brainliest to the most accurate one. universalizing religion definition ap human geography Josef and Mai divided some stamps between themselves. Josef got 60% of the stamps. If Josef received 500 more stamps than Mai, how many stamps did Josef receive? The total cost of going to the theatre for 18 people is 803.16.How much will each person need to pay to cover the total cost? In Joe's fish tank, there are 5 goldfish and 12 snails. How does the ratio 5:17 describe Joe's fishtank?Choose the correct answer below.O A. The ratio 5:17 compares the number of snails to the number of goldfish.OB. The ratio 5:17 compares the number of snails to the total number of animals.OC. The ratio 5:17 compares the number of goldfish to the total number of animals.OD. The ratio 5:17 compares the number of goldfish to the number of snails. The time, t, it takes a runner to cover a specified distance is inversely proportional to the runner's speed. If Jann Avery runs at an average of 6 miles per hour, shewill finish a race in 2. 1 hours. How long will it take Jackie Donofrio, who runs at 4 miles per hour to finish the same race? Select each correct answer, you would really help me out! 3. Tata is a new member of Teratai English Club. She introduced herselfTata : Ladies and gentlemen A student graphed the position of a cart during a 7-second time interval.Which statement best describes the cart? A. The cart was stationary except for a short burst of acceleration of 0.5 m/s. B. The cart accelerated at a constant rate of approximately 0.8 m/s. C. The cart was stationary with a velocity of 0 m/s for the entire 7 seconds. D. The cart moved at a constant velocity of 0.5 m/s for the entire 7 seconds. Calculator15What is the value of x?93xBEnter your answer in the box2x-1X = An investor purchased 50 shares of stock in a company in 2015. At the time the investor purchased the stock, the value of thestock was priced at $5 per share. The investor sold all 50 shares of stock in 2019. The value of the stock at the time of salewas $6.50 per share. What is the capital gain the investor earned on this investment? (1 point)O $75O $325O $50O $250 If anyone could plz help ill really appreciate it Describe the internal control features that were missing that enabled the employee to commit this fraud.Describe what actions the employer should take to prevent this kind of fraud from happening in the future.High-Flying Auto Dealer Employee Steals $10M For LuxuriesThe scheming employee was able to electronically transfer funds from the company's online account to her own personal account and use the funds to buy four homes, 10 vehicles, Louis Vuitton luggage, jewelry, $30,000 in pre-paid travel funds and stow away more than $20,000 in savings, court records show. Authorities said she covered up the thefts with a series of fake transactions as well as listing phantom cars in inventory so they could be recorded as assets. help me solve please Operating under the color of law refers to _____. the requirement that jails treat incarcerated individuals equally the person bringing a legal action against a defendant the legal authority that some persons have over citizens the use of police force against a person of color Identify the ways that the Belgian colonial government justified and enforced their preferential treatment of Tutsi over Hutu in Rwanda.