Answer:
To concentrate the molecule of interest.
Explanation:
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The "capture" antibody binds to and retains the analyte from the sample while the rest of the matrix is rinsed away. An enzyme-conjugated "detector" antibody, raised against a different epitope upon analysis, is added to the plate.
What is Elisa Technique?ELISA stands for enzyme-linked immunosorbent assay. It is a commonly used analytical biochemistry assay. The assay uses a solid-phase type enzyme immunoassay to detect the presence of a ligand in a liquid sample using antibodies directed against the protein to be measured.
It is a plate-based assay technique designed for the detection and quantification of soluble substances such as peptides, proteins, antibodies and hormones. The antigen, called a target macromolecule, is immobilized on a solid surface called a microplate and then complexed with an antibody that is bound to a reporter enzyme.
There are many types of ELISA such as Sandwich ELISA. It measures the antigen between two layers of antibodies called capture and detection antibodies. The target antigen must have at least two antigenic sites capable of binding to the antibody
Thus, the "capture" antibody binds to and retains the analyte from the sample while the rest of the matrix is rinsed away. An enzyme-conjugated "detector" antibody, raised against a different epitope upon analysis, is added to the plate.
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Help, this looks simple yet I am confused.
Sarah, who is 15 years old, is performing a step challenge to strengthen her cardio-respiratory endurance. She commences by timing her resting heart rate for 6 seconds, then adds a 0 to the end of the number of heartbeats she counts . Sarah then after times her exercise heart rate for 6 seconds, then adds a 0 to the end of the number of heartbeats she counts, after she completes her 3-minute step challenge. She then rests for 1 minutes and after that minute she times her recovery heart rate for six seconds, then adds a 0 to the end of the number of heartbeats she counts. What are Sarah's heart rates throughout her resting, exercise, and recovery heart rate?
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Example: His resting heart rate is 7 beats throughout 6 seconds, add a 0 to the 7, which makes her resting heart rate 70.
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Questions:
Resting Heart Rate: __
Exercise Heart Rate:__
Recovery Heart Rate:__
please help
Your example shows that you add a 0 to the end of how many heartbeats you get throughout 6 seconds which was 7 so that would give you 70, however in Sarah's example it doesn't tell us how many times her heartbeats in 6 seconds so I can't give you the answer, but if that's how the question is supposed to be then the answer would be 60 for all.
I hoped that helped! Let me know if you have any questions!
Have a great day/night! :)
Which physiological demands are highly emphasized in baseball?
(Select all that apply.)
Answer:
Reaction time
Aerobic endurance or muscular endurance
Answer:
The physical demands of baseball are very specific to the sport. Baseball requires repetitive high intensity explosive movements, with time for full-recovery between plays, and all this spread out over nine innings
Once you can appreciate pop music as a form of _____________, rather than a guide for life, you can have fun with it while still accepting and appreciating yourself.
A.
religion
B.
entertainment
C.
rules
D.
advice
Answer:
D
Explanation:
how old do you have to be to get your nipples pierced
What is the best definition of detoxification?
A.
gradually but completely withdrawing from alcohol or drugs
B.
a lifelong commitment to living alcohol-free or drug-free
C.
acknowledging a person's problem with alcohol or drugs
freezer burn a grainy whitish or brownish spot on foods is caused by
too much moisture inside the food
moisture loss or ice crystals
temperature change in the freezer
ice crystal evaporating
Answer:
Moisture loss second one
Explanation:
the american academy of pediatrics recommends that all breastfed infants up to six months old be given what kind of supplement?
how can turkey be prepared for thanksgiving dinner
In terms of ethnicity, the __________ ideal has made its way around the globe and questioned the value of anything different.
A.
Hispanic
B.
German
C.
eastern
D.
westernized
Answer:
A
Explanation:
i am on the test
when performing hands-only cpr, how many times should you push in the center of the chest during a one minute period?
Answer:
Push down in the center of the chest 2-2.4 inches 30 times. Pump hard and fast at the rate of 100-120/minute, faster than once per second
Explanation:
you are spending some quality time with your grandparents and you notice your grandfather’s face drooping and he is having difficulty speaking. what should you do?
If you notice your grandfather's face drooping and he is having difficulty speaking, it could be a sign of a stroke, which requires immediate medical attention. In such a situation, it is important to act quickly by following these steps:
Call 911: Dial emergency services or the appropriate emergency number in your country to request immediate medical assistance. Inform the operator about the symptoms your grandfather is experiencing and follow their instructions.
Stay with your grandfather: Ensure that your grandfather is in a safe and comfortable position. Do not leave him unattended.
Note the time: Pay attention to when the symptoms started or were first noticed. This information can be helpful for medical professionals in determining the appropriate treatment.
Do not give anything to eat or drink: Refrain from giving your grandfather any food, beverages, or medication unless advised to do so by medical professionals.
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what part of a nephron is responsible for reabsorbing all the nutrients and most of the water?
Answer:
the proximal convoluted tubule
Explanation:
Filtered fluid enters the proximal tubule from Bowman's capsule. Many substances that the body needs, which may have been filtered out of the blood at the glomerulus, are reabsorbed into the body in this segment.
which form of contraception may prevent a woman from becoming pregnant after she has discontinued its use for up to one year?
Answer:
The hormone progestin in the birth control shot
Explanation:
A snakebite should be treated by elevating the bite.
True
False
Considering that overpopulation is a national concer, what is your take regarding unplanned pregnancies among young adults? How do you ascertain if one is ready to start a family?
Answer:
Eighty-six percent of pregnancies and you're both excited and eager to start a family, and you're feeling settled financially
Explanation:
whose responsibility is it to keep ladders in good condition?
list three eating habits that can help keep your digestive system healthy. Why is excersise important for digestive health?
Answer:
Consume plenty of fiber, limit fatty foods, and drink water. It stimulates peristalsis which can prevent constipation.
Physical activity increases blood flow to the muscles in the digestive system, which massage our food along the digestive tract – a process known as peristalsis – causing them to work more quickly and effectively. Research also suggests that exercise affects the balance of bacteria in the gut.
Explanation:
a patient is on a mechanical soft diet. which food item choice by the patient will cause the nurse to intervene?
Given our understanding of this diet type and the reasons behind it, we can confirm that a food item choice by the patient that will cause the nurse to intervene is red meat.
A mechanical soft diet is one in which the patient is allowed to eat solid food, however, the food must be soft and require a minimal amount of chewing. This diet is recommended for patients that have any form of digestive tract illness that may cause difficulty when chewing or swallowing food.
Red meat would cause the nurse to intervene as it presents a great risk for choking or other complications and is prohibited by this diet.
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Mr. Johnson has a piece of pipe 5 1/3 feet long. He decides to cut it into 3 equal pieces to use in making a railing for his porch. How long should he cut each piece?
A 1 7/9
B 4 1/2
C 10 1/2
D 16
Answer:
A 1 7/9
Explanation:
5 1/3 can be re written as 16/3 (as a fraction)
16/3 divided by 3 is 16/9
1 7/9 can be re written as 16/9 (as a fraction)
16/9=16/9
are you a moderately to severely immunocompromised individual?
Answer:
People who are moderately to severely immunocompromised make up about 3% of the adult population and are especially vulnerable to ilnesses because they are more at risk of serious, prolonged illness.
Explanation:
hipaa applies to my delivery of prescriptions on behalf of instacart because
The application of HIPAA to the delivery of instacart prescriptions is due to the customer's lawful status as a business associate of HIPAA when obtaining a prescription.
HIPAA is the acronym for the Health Insurance Portability and Accountability Act. This is an act passed by the United States Congress that called for a national standard to be implemented with the goal of protecting sensitive patient information regarding health from being disclosed without consent. It is comparable to the doctor-patient confidentiality agreements practiced by most health professionals.
Instacart Rx is an entity that is covered by HIPAA regulations. Upon being so, Instacart is considered a business associate of HIPAA along with all of its customers. Therefore, all workers and customers must comply with HIPAA regulations for the disclosure of information pertaining to patients.
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Give atleast 5 characteristics of a healthy child pls pls pls help me!! i will follow you
Answer:
. Self-Consciousness
growth and development
independence
dependency, vulnerability,
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Day-in-the-Life Scenario
You will now use your accumulated knowledge about the exercise physiologist career and the musculoskeletal system to design a scenario that provides a realistic understanding of the profession. Of course, you may also use additional research, but your scenario must be original.
Answer the following questions:
1. In what setting are you located?
2. Who are your most regular patients?
3. What is the problem the client is presenting, e.g., weak heart due to perfusion/bypass, lack of range of motion due to knee injury, etc.?
4. What kind of very general activities would you prescribe?
5. What is the description of at least one tool from the list in the lesson, along with an.
interpretation of the data it produces? (You may need to do additional research, so be sure to include a list of Works Cited for your teacher.)
6. What is a situation in which there is a potential hazard for one of your clients, and how did you respond to that situation? This could be a heart attack, a fainting spell, dangerous equipment, etc.
7. What is a situation in which you had to work with another member of the health team in order to get the best outcome for your patient?
8. What is a situation in which you had to educate the patient on what kind of exercise you were providing and why?
9. What ethical situation came about in which you had to check out the ASEP Code of Ethics, or, if the URL was unavailable, base your reaction on your own understanding of past ethical discussions?
10. What situation occurred in which you had to explain certification and licensure to the client?
You can combine all of these sections into interaction with just one client or describe a whole day with various clients.
Answer:
6454
Explanation:
Why is importan have a good relationship with others?
Answer:Healthy relationships can decrease stress and lead to a longer life. Research suggest that the social and emotional support from a committed relationship or strong friendship can be a buffer against stress. Studies also suggest many biological benefits, including lower blood pressure and stronger immune systems.
Explanation:
which of the lobes of the brain contains the gustatory cortex?
Answer:
The insula, which lies deep to the temporal, parietal, and frontal lobes, contains the gustatory cortex responsible for the perception of taste.Explanation:
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Synthetic drugs are safer than other drugs because they are made where?
In nature
At the grocery store
In your neighborhood
In a laboratory
what is the highest risk of exposure to hazardous drugs?
Because the hazardous substances are in their most concentrated form when they are being prepared, this is the phase of the process that has the greatest potential for exposure to the substances.
What are hazardous drugs?A drug is considered to be hazardous if it exhibits one or more of the following characteristics in experimental animals: genotoxicity, carcinogenicity, teratogenicity, or fertility impairment; serious organ or other toxic manifestation at low doses; or serious organ or other toxic manifestation at low doses.
In Group 1, we have the anticancer medicines (roughly, chemotherapy medications). Group 2 consists of potentially dangerous pharmaceuticals that are not antineoplastic, and Group 3 includes medications that may inhibit fertility in either men or women, as well as those that may pass into breast milk.
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what is lifestyle disease how this affect and individual like you
Answer:
atherosclerosis, heart disease, and stroke; obesity and type 2 diabetes; and diseases associated with smoking and alcohol and drug abuse
Explanation:
injuries to the ligaments around a joint are called
Answer:
Hey mate.....
Explanation:
This is ur answer....
A sprain is an injury to the ligaments which are soft tissue structures that stabilize a joint.Hope it helps!
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