The enzyme dihydrofolate reductase (DHFR) normally resides in the cytosol, and it can be imported into mitochondria by appending a mitochondrial signal sequence. However, when this modified DHFR is incubated with methotrexate, which is a substrate analog that binds tightly to the active site, the modified DHFR is no longer imported. Propose an explanation for this finding.

Required:
a. Consider a transmembrane protein that spans the inner nuclear membrane. Suggest a pathway by which such a protein could reach its destination.
b. Clathrin can assemble with multiple types of adaptors to create coated vesicles. Explain why this multiplicity of adaptors is useful to the cell

Answers

Answer 1

Answer:

Within the mitochondria, the import takes place post-translationally. The imported protein must be in an unfolded condition so that it can go through the mitochondria's import machinery. In the existence of methotrexate, the bound DHFR moiety remains in the folded state, and thus, stays on the surface of mitochondria.

It is witnessed that the presequence must be of the length of 55 amino acids to cover both the membranes of mitochondria and must be of about sixty amino acids length to associate with the mhsp70. If the presequence of this methotrexate bound DHFR is of seventy-five amino acids in length, it can associate with mhsp70 within the matrix, to initiate the ATP reliant import within the mitochondria. Due to the presence of various endocytic and intracellular sorting signals, multiple adaptors are essential.


Related Questions

A white-tailed deer cannot make its own food and must consume other organisms
for its nutrition. Therefore, the white-tailed is considered a (an)
A)heterotroph.
B)producer.
C)chemotroph.
D)autotroph. .

Answers

Answer:

A) Heterotroph

Explanation:

White tailed deers are consumers and do not produce their own food. Most mammals can’t either. Putting in the heterotroph category.

Hope this helps, good luck!

The answer is option A


HELP PLSSS, IM SO CONFUSED

Answers

4. DNA is something you’re born with and cannot change. it is designed specifically to you. it’s your “blueprint”

5. 94,696.9696- miles

In guinea pigs, the color of the fur is determined by a single gene with two alleles control. The brown allele (B) is dominant over the white allele (b). Two heterozygous guinea pigs are crossed. Draw a genetic diagram with the genotypic, phenotypic and ratio information of the P₁ and F ₁ generations of this cross.​

Answers

Answer:

       B          b

B      BB       Bb

b       Bb      bb

Genotype:

ratio - 1:2:1

1 BB = 25%

2 Bb = 50%

1 bb = 25%

Phenotype:

ratio - 3:1

3 Brown: 75%

1 White:25%

HOpe this helps

Define Vascular tissue?

Answers

Answer:

Vascular tissue is a complex conducting tissue found in vascular plants that is made up of several cell types. The xylem and phloem are the two main components of vascular tissue. Internally, these two tissues carry fluid and nutrients.

OAmalOHopeO

Two Bio 45 students are having a disagreement about renal function. Dan says that the kidneys work harder when you eat a high-salt diet, whereas Peter says that they work harder when you drink lots of water.

Answers

The kidney works harder when you drink lots of water, no water = kidney failure.

which of the following occurs during the process of asexual reproduction
Mitosis only
meiosis only
Mitosis and meiosis
none of the these​

Answers

Answer:

Explanation:

Reproduction may be asexual when one individual produces genetically identical offspring, or sexual when the genetic material from two individuals is combined to produce genetically diverse offspring. Asexual reproduction occurs through fission, budding, and fragmentation.

In mutualism, both organisms participating benefit from the relationship.
True or False?

Answers

Answer:

true

Explanation:

Dr. Nachman and his colleagues collected rock pocket mice across 35 kilometers of the Arizona Sonoran Desert, which included both dark, rocky lava outcrops and light, rocky, granite areas. They recorded substrate color, and coat-color frequencies for each location. Each site was separated from any of the others by at least eight kilometers. The researchers trapped a total of 275 mice. Their data are summarized below:

Collecting Site Substrate Color Number of Mice Phenotype
Light Dark
1 Dark 22 3 19
2 Light 80 74 6
3 Light 22 19 3
4 Dark 28 3 25
5 Light 58 25 33
6 Dark 65 9 56


Required:
a. Calculate the overall frequencies of light-colored mice and dark-colored mice caught on light-colored substrates.
b. Calculate the overall frequencies of light-colored mice and dark-colored mice caught on dark-colored substrates.
c. Using the Hardy-Weinberg equation and data from the table above, determine the frequency of the three genotypes on the light, rocky, granite substrate. For genotype frequencies use three decimal places and for calculations using the allele frequencies, you must round the allele frequencies to two decimal places.

Answers

Complete question

Dr. Nachman and his colleagues collected rock pocket mice across 35 kilometers of the Arizona Sonoran Desert, which included both dark, rocky lava outcrops and light, rocky, granite areas. They recorded substrate color, and coat-color frequencies for each location. Each site was separated from any of the others by at least eight kilometers. The researchers trapped a total of 275 mice. Their data are summarized below (numbers have been slightly modified for the purposes of this worksheet).

Field Data Summary

Collecting Site Substrate Color Number of Mice Phenotype Light Dark

1 Dark 22 3 19

2 Light 80 74 6

3 Light 22 19 3

4 Dark 28 3 25

5 Light 58 25 33

6 Dark 65 9 56

1. Calculate the overall frequencies of light-colored mice and dark-colored mice caught on light-colored substrates. [to 3 decimal places]

2. Calculate the overall frequencies of light-colored mice and dark-colored mice caught on dark-colored substrates. (to 3 decimal places)

3. Using the Hardy-Weinberg equation and data from the table above, determine the frequency of the three genotypes on the light, rocky, granite substrate. For genotype frequencies use three decimal places and for calculations using the allele frequencies, you must round the allele frequencies to two decimal places.

4. Using the Hardy-Weinberg equation and data from the table above, determine the frequency of the three genotypes on the dark, rocky lava substrate. (to 3 decimals). For genotype frequencies use three decimal places and for calculations using the allele frequencies, you must round the allele frequencies to two decimal places.

5. Which fur color seems to have the greatest overall selective advantage? Use data collected from both dark-colored and light- colored substrates to support your answer.

6.What environmental change gave a selective advantage for one coat color over another?

7. In a separate study, 76 rock pocket mice were collected from four different, widely separated areas of dark lava rock. One collecting site was in Arizona. The other three were in New Mexico. Dr. Nachman and colleagues observed no significant differences in the color of the rocks in the four locations sampled. However, the dark-colored mice from the three New Mexico locations were slightly darker than the dark-colored mice from the Arizona population. The entire Mc1r gene was sequenced in all 76 of the mice collected. The mutations responsible for the dark fur color in the Arizona mice were absent from the three different populations of New Mexico mice. No Mc1r mutations were associated with dark fur color in the New Mexico populations. These findings suggest that adaptive dark coloration has occurred at least twice in the rock pocket mouse and that these similar phenotypic changes have different genetic bases. How does this study support the concept that natural selection is not random?

8. To determine if the rock pocket mouse population is evolving, explain why it is necessary to collect fur color frequency data over a period of many years.

Answer:

1)

frequencies of light-colored mice  ≅ 0.74 frequencies of dark-colored mice  ≅ 0.26

2)

frequencies of light-colored mice ≅ 0.13 frequencies of dark-colored mice ≅ 0.87

3)

p² ≅ 0.02 q² ≅ 0.74 2pq ≅ 0.24

4)

q² = 0.13 p² = 0.4 2pq = 0.46

5) The dark-colored fur seems to have the greatest overall selective advantage

6) Dark lava changed the color of the substrate, from light to dark.

7) Because to produce dark color, animals from the different regions suffered different mutations that drove them to have almost the same dark fur color.

8) To see if the mice population is evolving. If it is, there will be a change in the allelic and genotypic frequencies over the years. If it is not evolving, the frequencies will keep equal through the years.

Explanation:

Due to technical problems, you will find the complete explanation in the attached files                  

Some plant seeds have special spiky barbs that can easily get tangled in an animal's fur. The spiky structure of these seeds most likely benefits a plant by

A. keeping animals far away from the parent plant.

B. keeping the seeds from being moved away from the parent plant.

C. attracting pollinators with their bright color and fruity smell

D. helping the seeds to be scattered in new areas to grow.

Answers

D. helping the seeds to be scattered in new areas to grow
D. helping the seeds to be scattered in new areas to grow.

Brendan made a diagram to compare and contrast the benefits of two types of social behavior.

The diagram shows 2 overlapping circles labeled Flocking and Herding. There is a z in Herding.

Which description belongs in the area marked "Z"?

reproduction
protection
foraging
hydrodynamic efficiency
please help this is an exam and its timeddddd

Answers

Answer:

Which description belongs in the area marked Z? Protection.

Explanation:

Have a great summer.

Herding is refer to as the act of bringing individual animals together into a group, maintaining the group, and moving the group from place to place or any combination of those.  The description belongs in the area marked "Z" is foraging. Thus, the correct option is C.

What is flocking?

A flocking is refer to as the large number of birds, especially those gathered together for the purpose of migration.  For example, a large number of animals, especially sheep or goats kept together and migrate.

Foraging behavior includes all the methods by which an animal acquires and utilizes sources of energy and nutrients. This also includes the location and resources consumption, as well as their recapture and storage, within the context of the larger community.

For more information regarding foraging, refer:

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How is waste (and the accumulation of it) not only harming wildlife, but ourselves?

Answers

Not many people knew about this, but many of our waste go back into the rivers and water companies collect that water and sell it back to us!
And it has recently been on the news that a pregnant woman had micro plastics found inside the umbilical cord! Everything we drink and eat, has an 100% chance of having mini micro plastics.

Conver 3 miles to feet

Answers

Answer:

1mile = 5280ft

3miles = 3x5280 = 15840ft

Answer:

the answer is 15840 feets

Which statement is true? (1 point)
O Fossils cannot provide information about relatedness of life forms.
O Fossils can provide information about when life forms existed.
O Fossils cannot provide information about life cycles and behaviors.
O Fossils can provide information about the entire evolutionary process of life forms.

Please help! I will give you Brainly !

Answers

Answer:

1. fossils can provide information about when life forms existed

2.3

3.they can show false relationships between organisms that are not reflected in the DNA

4.Living things have adapted and changed over time for specific, discoverable reasons

5.This suggests that all of these species share a common ancestor

Explanation:

Fossils give us information about how animals and plants lived in the past.

Fossils can provide information about when life forms existed.

Fossils can provide information about the entire evolutionary process of life forms.

What are Fossils?Fossil of an animal or plant has been preserved in Earth’s crust. It is the primary source of information about the history of life on Earth.Fossils are the undecayed remains of the living organisms. These remain preserved inside the lining of sediments.

Thus, we can conclude that the correct options are A and D.

You can learn more about fossils here:

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Artificial wetlands ________. are the major program for replacing lost natural wetlands purify water for use as bottled drinking water are a source of arsenic contamination in Bangladesh are created using xeriscaping methods can help purify water and also provide wildlife habitat

Answers

Artificial wetlands can help purify water and also provide recreational opportunities. They are the major program for replacing lost natural wetlands purify water for use as bottled drinking water are a source of arsenic contamination in Bangladesh are created using xeriscaping methods can help purify water and also provide wildlife habitat.

_______ are large geographical areas that have similar climate and weather patterns.

a.Symbioses
b. Zones of tolerance
c. Biomes
d. Communitie

Answers

Answer:

i think its c.............

Answer:

The answer is C. Biomes

Explanation:

Large areas with similar climate and weather are called biomes.

How can we control the laser light show to stop it from hitting the crowd

Answers

Answer:

just turn it off

Explanation:

Using mirrors to reflect the lasers and “bounce” them to other sides of the room

What are two things we need to consider to make living off Earth possible?​

Answers

Oxygen and Food? Tell me if that ain’t it :)

how scientists are able to determine climate information by just looking at tree rings? (how does it work?)

Answers

Explanation:

Because trees are sensitive to local climate conditions, such as rain and temperature, they give scientists some information about that area's local climate in the past. For example, tree rings usually grow wider in warm, wet years and they are thinner in years when it is cold and dry.

Answer:

Explanation:

Climate scientists compare the tree growth records to local weather records. For locations where a good statistical match exists between tree growth and temperature or precipitation during the period of overlap, the ring widths can be used to estimate past temperature or precipitation over the lifetime of the tree.

The diagram shows the human alimentary canal. In which parts does peristalsis take place?​ plss be sure abt it

Answers

Answer:

i think answer is 3 and 4

Explanation:

peristalsis is located at esophagus and in our digestive system

The parts where peristalsis will take place are ; ( C )  parts 3 and 4

Peristalsis is a series of muscle contraction occurring in the digestive system of Humans also these series of muscle contraction can be seen in tubes connecting the human kidney and bladder as well.  

From the diagram parts 3 ( esophagus )and 4 ( intestines ) are part of the human digestive system and peristalsis is very evident in these regions in the human body.

Hence we can conclude that The parts where peristalsis will take place are  3 and 4

Learn more : https://brainly.com/question/19838720

What is happening in these images?

Answers

there aren't any images lol

3. If you were looking at a cell that had all of its chromosomes lined up down the middle of
the cell, what phase must it be in?
1. Telophase
2. Prophase
3. Metaphase
4. Anaphas

Answers

Answer:

3. Metaphase

Explanation:

Metaphase is the stage in the cell cycle where the chromosomes line up in a line down the middle of the cell (some people call it the metaphase plate).

The way I like to remember it is: Metaphase is in the Middle.

I hope this helps! :D

Answer:

I'm sorry hindi ko po alam

Please answer only if you know this is a final 

Answers

Answer:

ecosystem

Explanation:

its also nice to keep a _______ in my purse

Answers

Answer:

its also nice to keep a __phone_____ in my purse

Explanation:

Chemical energy in the form of food is changed into what?

Answers

Food is the most important source of chemical energy for living things. The chemical energy in food is converted, or changed, by the body into moving mechanical energy and heat energy. People have invented ways to use the chemical energy of substances to power machines.

Energy is transferred from the Sun to Earth many by

Answers

Energy is transferred from the Sun to Earth mainly by [tex]\sf\purple{electromagnetic\:waves\:or\:radiation}[/tex].

D. electromagnetic waves.

[tex]\circ \: \: { \underline{ \boxed{ \sf{ \color{green}{Happy\:learning.}}}}}∘[/tex]

Scientists observed that the populations of top-level consumers in a particular ecosystem were rapidly decreasing. Further studies revealed that there was also a decline in producer productivity. Which other changes did the scientists most likely observe in the ecosystem?


increased producer diversity


decreased population size at all levels


decreased primary consumer populations only


increased primary and secondary consumer diversity

Answers

Answer:

The correct answer is - decreased population size at all levels.

Explanation:

A decrease in the productivity of producers of an ecosystem will lead to a decrease in all other organisms of the ecosystem including top-level consumers to the bottom-level producers due to the fact that producers provide energy and food to primary consumers.

It is because they are the only organisms that convert the sunlight to usable energy and food that transfer from one trophic level to other, so if the population of producers or their productivity will decrease then there will be decreased population size at all levels.

In fruit flies, the phenotype for eye color is determined by a certain locus. E indicates the dominant allele and e indicates the recessive allele. The cross between a male wild-type fruit fly and a female white-eyed fruit fly produced the following offspring.

Wild Type Male Wild Type Female White-eyed Male White-eyed Female Brown-eyed Female
F1 Generation 0 45 55 0 1

The wild-type and white-eyed individuals from the F1 generation were then crossed to produce the following offspring.

Wild Type Male Wild Type Female White-eyed Male White-eyed Female Brown-eyed Female
F2 Generation 23 31 22 24 0

Required:
a. Determine the genotypes of the original parents (P generation) and explain your reasoning.
b. Use a Chi-squared test on the F2 generation data to analyze your prediction of the parental genotypes.

Answers

Answer:

The genotypes of the original parents are

Male: X⁺Y   Female: X⁻X⁻

The Chi-square analysis leads us to accept the prediction of the parental genotypes.

Explanation:

Due to technical problems, you will find the complete explanation in the attached files.

(a) The genotypes for the original parents (P generation) are

Male: X⁺Y  

Female: X⁻X⁻

(b) The prediction of the parental genotype is accepted by the Chi-squared test analysis.  

What is the eye color pattern in fruit flies?

Drosophila Melanogaster is a fruit fly with abnormally big, brightly colored eyes.

The range in color from red to sepia to white reveals a lot about the genetic makeup of the fly.

(a)  E = + ⇒ Dominant allele coding for wild-type eyes

     e = - ⇒ Recessive allele coding for white eyes

Now, Crossing the : male wild-type with a female white-eyed

(Parental)   =  X⁺Y x X⁻X⁻

(Phenotype) =   wild   white

(Gametes)  =    X⁺ Y X⁻ X⁻

(Punnett square)  =  X⁺ Y

X⁻   =  X⁺X⁻  X⁻Y

X⁻   =  X⁺X⁻   X⁻Y

In (F1)generation = 2/4 = 1/2 = 50% of the progeny are expected to be wild-type females.

And 2/4 = 1/2 = 50% of the progeny are expected to be white-eyed males.

Thus, the percentages are almost 50:50, as the expected ones.

So, the genotypes of the original parents are

Male:      X⁺Y

Female:  X⁻X⁻

(b) Using Chi-squared test :

Observed fruit fly type:

Wild Type Male - 25

Wild Type Female - 31

White-eyed Male - 22

White-eyed Female - 24

Expected fruit fly type:

Wild Type Male - 25

Wild Type Female - 25

White-eyed Male - 25

White-eyed Female - 25

Now, chi square [tex]= \sum\dfrac{(O- E)^2 }{E}[/tex]

By putting numbers in the formula, we are getting

Wild Type Male - 0.16

Wild Type Female - 1.44

White-eyed Male - 0.36

White-eyed Female - 0.04

Now, X² = 0.16 + 1.44 + 0.36 + 0.04 = 2

Critical value = 7.82

X² (2) < critical value (7.82)

Thus, this is the evidence to accept the given hypothesis.

Learn more about fruit flies here:

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¿De qué manera pueden complementarse la
teoría Sintética y la teoría de los Equilibrios
Puntuados?

Answers

La teoría sintética

El redescubrimiento en 1900 de la teoría de la herencia de Mendel por el botánico y genetista holandés Hugo de Vries y otros llevó a un énfasis en el papel de la herencia en la evolución. De Vries propuso una nueva teoría de la evolución conocida como mutacionismo, que esencialmente eliminó la selección natural como un proceso evolutivo importante. Según de Vries (a quien se unieron otros genetistas como William Bateson en Inglaterra), en los organismos tienen lugar dos tipos de variación. Una es la variabilidad "ordinaria" observada entre los individuos de una especie, que no tiene consecuencias duraderas en la evolución porque, según de Vries, no podría "conducir a una transgresión de la frontera de la especie [es decir, al establecimiento de nuevas especies] incluso en las condiciones de la selección más rigurosa y continua ". El otro consiste en los cambios provocados por mutaciones, alteraciones espontáneas de genes que dan lugar a grandes modificaciones del organismo y dan lugar a nuevas especies: “La nueva especie se origina así de repente; es producido por el existente sin ninguna preparación visible y sin transición ”.

Muchos naturalistas se opusieron al mutacionismo y, en particular, los llamados biometristas, liderados por el estadístico inglés Karl Pearson, quien defendió la selección natural darwiniana como la principal causa de la evolución a través de los efectos acumulativos de variaciones individuales pequeñas y continuas (que los biometristas se supone que pasa de una generación a la siguiente sin estar limitado por las leyes de herencia de Mendel).

There are marked differences in the type of organisms found at four different locations at the same tidal height along ta rocky shore. What might account for this

Answers

Answer and Explanation:

Many elements can be responsible for this, among them, we can mention the presence and absence of predators in the different places on the rocky coast. This is because predators can influence not only the number of organisms found, but also the types of organisms.

Another element that may be the cause of this variability is the availability of resources necessary for the life of these organisms, in addition, the impact of the waves on the rocks, can cause the variability of the organisms, since some may not be able to resist the impact of the waves.

Cardiogenic shock shock results when: The body loses an excessive amount of blood The body's blood vessels are dilated The left ventricle cannot pump enough blood to meet the body's demands Trauma results in temporary loss of the autonomic function of the spinal cord

Answers

Answer:

The left ventricle cannot pump enough blood to meet the body's demands

Explanation:

Cardiogenic shock is a problem that occurs when the heart cannot pump the adequate amount of blood to supply all organs. In this situation, the left ventricle, responsible for pumping oxygen-rich blood, cannot pump enough blood to tend the body's demands. This causes blood pressure to drop and oxygen in the tissues to be lacking. In addition, this situation can cause accumulation of fluid in the lungs. This is a very dangerous problem, being one of the main causes of myocardial infarction.

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