The size of the human genome is 3.2x109bp. The 3.2kb long fragment is to be amplified by Polymerase Chain Reaction (PCR) using the human genome as a template. How many cycles of PCR is necessary for the amplified target sequence at least 50% (by mass) of the total DNA in the reaction mixture. Explain how you arrived to this number.

Answers

Answer 1
20 cycles of PCR is necessary for the amplification target sequence

Mass is proportional to sequence length.

Let one base pair have mass M.

Thus,

[tex]3.2*10^9 bp[/tex] will have mass [tex](3.2*10^9) * M[/tex]

And the 3.2kb target will have a mass of

For the total amount of amplified target sequence to constitute 50% (by mass) of the total DNA in the reaction mixture, it has to be amplified to such an extent that the total mass of the amplicon matter is also [tex](3.2 * 109) * M[/tex]

PCR amplification happens geometrically, doubling the DNA target with each cycle.

Thus, if the number of cycles in N,

Then,

[tex](3.2 * 10^9) x M = 3.2 x 1000 x M x (2)N\\\\or 10^6 = (2)^N\\\\or 6 = N log2\\\\or N = \frac{6}{log2} = 20[/tex]

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This client has high blood pressure and a low heart rate. How does high blood pressure lead to a low heart rate

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the tissue of the heart may become thicker in an attempt to beat harder due to the increase in blood pressure. It's harder for this thickened tissue to conduct electrical impulses. As a result, your pulse might slow down because it takes longer to transmit electrical impulses

What are the metabolic pathways involved in maintaining blood glucose levels??

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The metabolic pathways involved in maintainance of blood glucose levels includes:

GlucoseGlycolysisGlycolysis Eicosanoid ReceptorGlycolysis Eicosanoid Receptor EnzymesGlycolysis Eicosanoid Receptor Enzymes Adenosine

In the body, the metabolism of glucose and other six carbon sugars starts with the catabolic pathway which is called glycolysis.

In the catabolic pathway, sugars are oxidized and broken down into pyruvate molecules.

Hence, the corresponding anabolic pathway by which glucose is synthesized is termed gluconeogenesis.

Read more about metabolic pathways

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DID I CLICK THE RIGHT ONE
help me lord

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Answer:

the answer is A

Explanation:

Hope this helps

The correct answer is C

According to a report by the American Broadcasting Corporation (ABC) on December 23, new research shows that the number of deaths in my country during the first and second waves of the new crown epidemic may be severely underestimated, especially among the elderly and the poor. The actual number may be It is 12 times higher than the official statistics, that is, more than 6 million people. According to the report, if this data is accurate, my country will become the country with the largest number of deaths from the new crown epidemic in the world so far, surpassing the 811,000 people in the United States.

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Answer:

It sounds like it would be one of the worst countries in the world for c-19. That sounds like it checks out, but could you provide a link?

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What are hematologic stains and their composition ?

Answers

Answer:

Hematology stains and reagents are essential for clinical and medical cytology research. ... Romanowsky stain solutions are used in hematology. They are composed of methylene blue, oxidative products of methylene blue (Azure A, Azure B, Azure C, and Thionin) and eosin dyes.

Explanation:

the distal end of the femur and the proximal of the tibia and fibula from the ___ joint

Answers

Answer:

syndesmosis

Explanation:

The inferior tibiofibular joint is a fibrous joint, precisely a syndesmosis. The articular surfaces of this joint are the triangular convex surface on the medial aspect of the distal end of the fibula and the reciprocally concave fibular notch on the distal end of tibia

Hope it helps

In which area would a veterinary scientist NOT consider the possibility of running across anthrax? A. Antarctica B. the Southeastern United States C. the United Kingdom D. Anthrax is found all over the world.

Answers

Answer:

D

Explanation:

Anthrax is found all around the world

Anthrax is found everywhere, so there is no place where a veterinary scientist would not consider the possibility of running across anthrax. The correct option is D.

What is anthrax?

An uncommon bacterial disease called anthrax is spread through mosquitoes, insects, and other animals. Victims may feel unwell, develop skin ulcers, or experience respiratory problems. Anthracis produces dry spores that can spread through the materials for a very long time after the first release.

Anthrax is thought to have originated in Egypt and Mesopotamia. Currently, it is present in the areas of central and South America, Africa, and Asia.

The three types of anthrax are:

cutaneous (skin)inhalation (lungs)gastrointestinal (stomach/intestine)

Therefore, the correct option is D. Anthrax is found all over the world.

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Answer:

to what

Explanation:

Answer:

•••• answer to what ••••

whats something that affects your social relationships in a positive way and why? Please help

Answers

Answer:

Something would be your clothes

Explanation:

everybody says that "first impression is the most important" and that also means your clothes beceause, you can have Einstein's brain and it doesn't matter at first glance it matters only how you look (clothes, hair, etc.)

is the following statement true or false? fracture that is not specified as displaced or not displaced should be coded to displaced

Answers

Answer:

True

Explanation:

In ICD-10-CM a fracture not indicated as displaced or nondisplaced should be coded to displaced, and a fracture not designated as open or closed should be coded to closed. While the classification defaults to displaced for fractures, it is very important that complete documentation is encouraged.

when determining the nature of a drug inquiry, how much detail required is also considered the ___ of the inquiry

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The determination of the amount of detail needed in a drug inquiry is known as the Scope of the inquiry.

A Drug InquiryIs established to acquire certain information on a drug. Can be used to check the effectiveness of a drug.

In a drug inquiry, the inquirer will need a variety of information to satisfy the purpose of that inquiry. The amount of information needed is known as the scope of the inquiry.

In conclusion, the answer is scope.

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DOES ANYONE KNOW PLEASE HELP ASAP !!!!!!

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Answer:

B. Formulary management

Explanation:

Formulary management is an integrated patient care process which enables physicians, pharmacists and other health care professionals to work together to promote clinically sound, cost-effective medication therapy and positive therapeutic outcomes.

8. According to the National Center on Elder Abuse, family members
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Answer:

True

Explanation:

True.
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Help pls
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Answers

Answer:

Macronutrients are the nutrients your body needs in larger amounts, namely carbohydrates, protein, and fat. These provide your body with energy, or calories. Micronutrients are the nutrients your body needs in smaller amounts, which are commonly referred to as vitamins and minerals.Feb 14, 2020

Explanation:

Answer:

I'm also stuck on that one

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Answer:

A hemostat to achieve matricectomy: the process of surgically or chemically destroying all or part of the base nail portion called the nail matrix.

1After the IRB reviews and approves your protocol, informed consent, and associated documents, you should receive:

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After approbation, you should receive written notification of the IRB decision and the approved versions of the protocol and informed consent forms. IRB reviews all research projects involving humans.

The Institutional Review Board (IRB) is a panel of experts designated to review biomedical investigations involving human subjects.

This Board (IRB) reviews all research projects before the step of data collection in order to approve (when appropriated), disapprove or modify them.

The main classes of IRB reviews include exempt, expedited, and full.

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Answer:

It's D

Explanation:

Hope this helps

ok here PLEASE HELP ME !!!!!

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Answer:

The answer is C

Answer:

I thought it was D-

Explanation:

a substance held capable of prolonging life indefinitely b(1) : cure-all (2) : a medicinal concoction

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Answer:

It's B

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May I get Braineist Pls?

Question 60 (5 points)
What is the minimum number of minutes of daily physical activity recommended by the Choosemyplate.gov?
са
10
b
75
ООО
С
30
d
60

Answers

Answer:

C) 30 minutes

Explanation:

The PAG (physical activity guidelines) recommends at least 30 minutes of physical activity in a daily basis.

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Answers

Answer:

Ascites (ay-SITE-eez) is when too much fluid builds up in your abdomen (belly). This condition often happens in people who have cirrhosis (scarring) of the liver. A sheet of tissue called the peritoneum covers the abdominal organs, including the stomach, bowels, liver and kidneys. The peritoneum has two layers.

Order: ABC 175 mg po. Stock: ABC 350 mg po scored tablets. How many tablets would the patient take per dose? _______________________

Answers

The number of tablets that the patient will take per dose is 0.5 tablets.

Using this formula

Number of tablets=Order in mg po /Stock in mg po

Where:

Order=175 mg po

Stock=350 mg po

Let plug in the formula

Number of tablets=175mg/350mg

Number of tablets=0.5 tablets per dose

Inconclusion the number of tablets that the patient will take per dose is 0.5 tablets.

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Chronic migraine sufferers should

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Answer:

People who suffer from chronic migraines should consult their doctor to get their opinion and a potential diagnosis. Migraine can be formed by pinched nerves, stress, or a potential disorder. If you have a migrain, in order to self treat it you should lay down in a dark room with a cool rag over your eyes. Too much movement can cause dizziness and in worse cases hallucinations.

Talk to their doctor and get the doctors opinion

Name three top priorities of the Medical Assistant?

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Take vitals
Assisting physicians during exams
Explaining procedures

The medical assistant's top three priorities are assisting the doctor during the examination, taking vitals, and explaining the medical procedure.

Three main top priorities of the Medical Assistant are,

1. Medical assistant assists the doctor during the patient's examination and follow all the instruction by the doctors.

2. Medical assistant takes all the vitals like Blood pressure and heart rate. The medical assistants take the blood samples from the patient and give injections.

3. Medical assistants gather all necessary information from the patient and prepare a record of the patient's medical condition and history.

Therefore, the medical assistant's top three priorities are assisting the doctor during the examination, taking vitals, and explaining the medical procedure.

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Needed this asap, what are the dangers of constipation​

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Answer:

The dangers of constipation are:

Swollen veins in your anus. Straining to have a bowel movement may cause swelling in the veins in and around your anus.Torn skin in your anus A large or hard stool can cause tiny tears in the anus.Stool that can't be expelled. Chronic constipation may cause an accumulation of hardened stool that gets stuck in your intestines.Intestine that protrudes from the anus Straining to have a bowel movement can cause a small amount of the rectum to stretch and protrude from the anus.
You could get swollen veins, torn skin which causes some stretch marks, and stool that can’t be removed

Is there any difference between premature and preterm babies??

Answers

Answer:

there is I think is this for science?

Preterm labor is labor that begins early, before 37 weeks of pregnancy. Labor is the process your body goes through to give birth to your baby. Preterm labor can lead to premature birth. Premature birth is when your baby is born early, before 37 weeks of pregnancy.

Please Help! I don't know if I coded this right or not. (Medical Coding & billing)

The physician reported a level 4 service. A complete EKG & Spirometry.

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Answers

Answer:

380-the real code this time

Explanation:

95×4=380

MY MESSAGE

MARK ME THE BRAINLIEST PLSSS

How does the stomach virus spread? I been 4 ft away from a infected person. But no contact with vomit. should I be fine?

Answers

Answer:

It depends on what type of virus it is but you should be fine as long  as you didn't make contact with them like using the same spoons or forks and stuff like that if its one of those viruses but some of them can be air born

Explanation:

It depends on which type of virus I hope it helps you

Maria recently adopted a vegan lifestyle. Which nutrients does she need to focus on to ensure that she does not develop deficiency symptoms

Answers

Answer:

Protein and iron

Explanation:

Protein slows down digestion so it will make you feel full. So she would not need to go get a high calorie snack afterward. Iron will help transport oxygen throughout the body.

What are the major things a urinalysis is looking for ?

Answers

A urinalysis is a test of your urine. It's used to detect and manage a wide range of disorders, such as urinary tract infections, kidney disease and diabetes.

The urine analysis is done to observe the presence of blood, sugar, amino acids, bile pigments, etc. The presence of these unwanted molecules explains the type of disease.

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Urine is formed in the nephron in the kidney. In humans, there are two kidneys, and each kidney has millions of nephrons. Nephrons are the structural and functional units of the kidney.

The blood is filtered at the nephrons, and the filtrate enters the nephron. Active transport returns necessary ions and molecules in the filtrate, such as glucose and amino acids, to the body. After the urine is formed, it is passed down to the urinary bladder through the ureter.

The blood cells are large, so they don't enter normally in the urine. If glucose, bile pigments, and blood are present in the urine, it shows that the kidney is not in good condition. Many other diseases happen if the kidneys don't work well.

Hence, urine analysis is looking for unwanted particles in the urine, such as glucose, blood cells, etc.

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