The use of restriction enzymes and electrophoresis to generate a map of the DNA molecule is known as

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Answer 1

The use of restriction enzymes and electrophoresis to generate a map of the DNA molecule is known as RESTRICTION MAPPING. This technique is used to map an unknown fragment of DNA.

Restriction enzymes are enzymes that cut a DNA fragment at specific nucleotides sites, thereby generally smaller fragments of DNA.

Restriction mapping is a strategy used to map unknown fragments of DNA by digesting them into smaller fragments and then identifying the specific sites of the DNA breakpoints.

Restriction mapping can then be used to create a diagram of fragments of DNA marked with specific sites where they are cut by specific restriction enzymes.

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Related Questions

What are valence electrons?

Answers

Answer: : a single electron or one of two or more electrons in the outer shell of an atom that is responsible for the chemical properties of the atom.

Explanation:

What are the differences between an onion and a whitefish cell

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During mitosis, the animal cell will have visible centrioles, where the plant cell will not. In thin section slides, you will also see that the onion cells are all dividing along the same axis, while in the whitefish embryos the plane of division appears to be random.

Onion are something that you can eat and grow on trees while whitefish cells are a part of whitefish.

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What is the main purpose for plants to conduct photosynthesis?

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Plants are autotrophs, which means they produce their own food. They use the process of photosynthesis to transform water, sunlight, and carbon dioxide into oxygen, and simple sugars that the plant uses as fuel. These primary producers form the base of an ecosystem and fuel the next trophic levels.

what describes metal for being malleable?​

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Malleable metals will bend and twist into numerous shapes when affected by a hammer, whereas non-malleable metals might break apart into pieces. Examples of malleable metals are gold, iron, aluminum, copper, silver and lead. ... No metal is completely malleable, and all will break when under extreme stress


4. Arterioles are the

a. Vein system.
b. Arteries system.
c. Arteries and vein system.
d. Arteries and blood system

Answers

Answer:

b. Arteries system

Explanation:

Which layer of the atmosphere is experiencing this storm?

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Answer:

Troposphere

Explanation:

Almost all weather occurs in the lowest layer of the atmosphere, called the Troposphere. The exception being large storms like hurricanes and large Thunderstorms. These storms have clouds with great vertical development and the tops of these storms go into the Stratosphere.

lymph is largely composed of water that has escaped from

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Escaped from the Blood Plasma’s

Which is the odd
one? Explain.

A. RR
B. Rr
C. Aa
D. aa

Answers

Answer:

d aa its not capitalized like the other ones

Explanation:

. A kidney cell is an example of which type of cell?
a. somatic cell
b. germ cell
c. sex cell
d. haploid cell

Answers

Answer:

A. Somatic cell

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Explanation:

Adaptational characteristics of himalayan animals​

Answers

Answer:

Thick layer of body fat or bubbler, to reduce body heat loss. Strong hooves for walking on rock slopes. Thick fur on paws for protection from cold while walking on snow.

Explanation:

1) Thick layer of body fat or bubbler, to reduce body heat loss. 

2) Strong hooves for walking on rock slopes.

3) Thick fur on paws for protection from cold while walking on snow.

Photosynthesis (Where does the electron that is excited by light is taken). What is the source of the electron, where does it end up?

Answers

Answer:

The source of the light electron is the sun and it is taken in by the chlorophyll contained in the chloroplasts.

Explanation:

High-energy electrons from NADH and FADH2, are passed into and along the____ The energy from the electrons moving down the chain is used to move H+ ions across the_____ H+ ions build up in the_____ space, making it_____ charged and making the matrix negatively charged. in the inner membrane. H+ ions move through channels of______ The ATP synthase uses the energy from the moving ions to combine ADP and phosphate, forming high-energy______​

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Answers:

High-energy electrons from NADH and FADH2, are passed into and along the   electron transport chain   .

The energy from the electrons moving down the chain is used to move H+ ions across the   inner membrane   .

H+ ions build up in the    intermembrane     space, making it    positively     charged and making the matrix negatively charged.

In the inner membrane, H+ ions move through channels of   ATP synthase   .

The ATP synthase uses the energy from the moving ions to combine ADP and phosphate, forming high-energy         ATP          .

what insects don't have piercing and sucking mouth parts?
.​

Answers

Answer:

lice

Explanation:

they bite and chew on their host skin

Insect mouthparts come in different forms. The two most common forms are the chewing and piercing-sucking types (moth and butterflies have a different, unique form of mouthparts). To determine what type of mouth an insect has, get a good hand lens (10 to 15x) or a small microscope and a bright light.

Which structure gives you voluntary control over urination?

Answers

Answer:

d. External urethral sphincter

Explanation: is correct. post protected

7)
.......... and....
Are weak forces of attraction.

Answers

Answer:

inter molecular forces and van der wall forces are weak forces of attraction.

Explanation:

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As a part of her homework, June make a flowchart about the steps involved in hearing the sound energy produced by wind chimes.

A flowchart with four steps. The first step is the rods vibrate back and forth. The second step is wind chime rods hit each other. The third step is sound waves are created. The fourth step is the sound travels into your ears.

Which statement best explains the error in June's flowchart?

The wind chime rods hit each other before they begin to vibrate.
Sound waves are created before the wind chime rods hit each other.
The sound of the wind chimes travels to your ear before the rods vibrate.
The spin of the rods causes sound energy, not its vibrations.

Answers

Answer:

A is the final answer........

in response to action potentials arriving along the transverse tubules, the sarcoplasmic reticulum releases:

Answers

It release carbon dioxide

what gives animal cells their shape and structure?

Answers

Answer: Animal cell's shape and structure are determined by the cytoskeleton.

What is the cytoskeleton?

The cytoskeleton is present in all animal cells, prokaryotic (bacterial) cells contain a very rudimentary version of it, while plant cells do not have one at all. It consists of several types of large molecules that provide physical support to the cell as well as internal transport along its length for organelles like endoplasmic reticulum (ER), mitochondria, vacuoles, etc. The three main components are microtubules, intermediate filaments and microfilaments which give rise to different structures within the cell. Microtubules are long cylindrical tubes made up of two protein subunits (alpha and beta tubulin) that form a hollow tube. These are the main components of the cytoskeleton, they provide support to the cell while also having transport capacity for organelles like endoplasmic reticulum (ER), mitochondria, vacuoles, etc. Microtubules are dynamic structures which means their state is constantly changing i.e., growing, shrinking or moving around depending on requirements of the cell.

who has a German shepherd? I want a dog and need some information form a dog owner.Plz help​

Answers

Answer:

That is the type of dog that police use to find drugs, look for people, etc.

Explanation:

In angiosperm double fertilization, one sperm nucleus fertilizes the ______ to form the diploid ______, and a second sperm nucleus fuses with the two nuclei in the central cell of the embryo sac.

Answers

Answer:

one sperm fertilizes the egg cell, forming a diploid zygote

Explanation:

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In angiosperm double fertilization, one sperm nucleus fertilizes the egg to form the diploid zygote, and a second sperm nucleus fuses with the two nuclei in the central cell of the embryo sac.

The egg is the female gamete in an angiosperm. It is a haploid cell that contains one set of chromosomes. The zygote is the fertilized egg. It is a diploid cell that contains two sets of chromosomes. The zygote will eventually develop into an embryo. The central cell is a cell in the embryo sac that contains two haploid nuclei.

Double fertilization is a unique feature of angiosperms. It is a type of fertilization in which two sperm nuclei fertilize two different cells in the embryo sac. The first sperm nucleus fertilizes the egg to form the zygote. The second sperm nucleus fuses with the two nuclei in the central cell to form a triploid cell. This triploid cell will eventually develop into the endosperm, which is a food reserve for the developing embryo.

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You are attempting to carry out a transposon mutagenesis experiment using the pRL27 system. The Donor strain you use was giving to you by a lab mate who has been culturing it continuously for several months. When you set up your control plates, you find that the Donor strain grows poorly on Luria DAP Agar. Your transformation efficiency is very poor for this attempt at mutageneis. What likely happened to the Donor strain

Answers

The most likely happened to the donor strain is that the transposon is active in the donor resulting in the gradual accumulation of mutations. The accumulation of mutations is promoted by the insertion of the transposon.

A donor strain is a bacterial strain that contains a mobilizable fragment of DNA called plasmid.

These strains (donor strains) exploit the transference properties of a conjugative plasmid to modify a particular recipient strain.

Transposons, also known as jumping genes, are mobile genetic elements that alter the genome of an organism by jumping and thus inserting them into genetic sequences (i.e., into protein-coding sequences).

In this case, the presence of a transposon in the donor strain affected the expression of many genes required for the donor strain in order to grow into Luria DAP Agar.

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Which part of the plant cell does photosynthesis occur? Please help

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Answer:

Photosynthesis occurs in the chloroplast of the plant cell.

Explanation:

Please help me!! ASAP

Answers

Answer:

B) Mushroom --> Heterotroph

C) Algae --> Autotroph

A) Autotroph --> Producer

D) Hetrotroph --> Comsumer

Explanation:

Mushrooms are decomposers because like other fungi, they break down dead and decaying matter to make their own food

Producers, such as plants and algae, acquire nutrients from inorganic sources that are supplied primarily by decomposers whereas decomposers, mostly fungi and bacteria, acquire carbon from organic sources that are supplied primarily by producers.

Primary producer; organisms, such as plants and phytoplankton, that can produce their own food through photosynthesis or chemosynthesis; also called autotrophs.

Consumers are also referred to as heterotrophs in contrast to autotrophs, which are the producers of the food chain. Consumers therefore include animals and heterotrophic bacteria and fungi. Plants that are carnivorous and therefore consume organic matter as well are regarded as both consumers and producers.

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Discuss 2 other ways that scientists believe
kingdoms should be divided.

Answers

Answer:

Plants and animals? ...The question says two OTHER ways, and I don't know what ways have already been listed.

Explanation:

The two main kingdoms in the animal kingdom are plants and animals.

Scientists also list four other kingdoms including bacteria, archaebacteria, fungi, and protozoa.

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A DNA microarray contains immobilized DNA that corresponds to different genes. The microarray is hybridized with fluorescently labeled DNA probes reverse transcribed from the control and experimental mRNA samples. The control probe is labeled with a red fluorochrome, while the experimental probe is labeled with a green fluorochrome. What will be the color of the spot representing a gene with moderately upregulated expression in the experimental sample relative to the control one

Answers

The color of the spot of a gene with moderately upregulated expression will be lime-green. A DNA microarray is a technique used to determine the expression level of many genes simultaneously.

A DNA microarray is a laboratory technique used to identify the genes that are being expressed in a cell.

A DNA microarray contains many tiny spots of specifically labeled cDNA samples referred to as probes, which can be hybridized by base complementarity to transcribed (mRNA) gene sequences.

In the DNA microarray, the probes can be fluorescently marked gene sequences.

After hybridization, the DNA microarray is then scanned in order to determine the level of gene expression of each mRNA that hybridizes with their corresponding probes on the slide.

In this case, green spots evidence genes that are being strongly expressed in the sample.

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Question 41 A basement membrane anchors Multiple choice question. A) muscle tissue to nervous tissue. B) epithelial tissue to connective tissue. C) connective tissue to muscle tissue. D) brain tissue to nervous tissue. E) blood cells to plasma.

Answers

Answer:

B) Epithelial tissue to connective tissue

Explanation:

different types of cells in your body look and function differently. the basis for these differences is:

Answers

Different types of cells look and function differently, the basis for these differences is DIFFERENT PATTERNS of gene expression. This process (differential gene expression) leads to cell specialization.

Gene expression refers to the process by which the genetic information flows from a DNA sequence (i.e., a gene) to an mRNA molecule and finally to a protein.

In somatic (body) cells, the differential patterns of gene expression lead to the generation of different proteins and ultimately to different types of cells (cell phenotypes).

In consequence, cells are specialized to perform specific functions because different genes are being expressed.

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they overcame by the blood of the lamb and the word of their testimony

Answers

Answer:

Revelation 12:11

Choose the correct response to this statement: Presynaptic neurons synapse on the ________ of the postsynaptic neuron.

Answers

Answer:

Cell or body dendrites

Explanation:

The cell which connects the whole body is called neuron cell and the tissue made up from the following is known as nervous tissue.

The correct answer to the following is left.

The synapse is made of the following:-

Cell endingDendrite

The signal transfer to the whole body is in form of electrical and chemical signals.

Hence, the correct answer is left to the postsynaptic neuron.

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what percentage of extracellular fluid is accounted for by interstitial fluid and plasma?

Answers

Answer:

80%

Explanation:

Other Questions
When people enjoy whatever they are doing, they report some characteristic feelings that distinguish the (1. pleasure) _______________ moment from the rest of life. The same types of feelings are reported in the context of playing chess, climbing mountains, playing with babies, reading a book or writing a poem. They are the same for young and old, male and female, American or Japanese, rich or poor. In other words, the nature of enjoyment seems to be (2. universe) _______________. We call this state of (3. conscious) _______________ a flow experience, because many people report that when what they are doing is (4. special) _______________ enjoyable, it feels like being carried away by a current, like being in a flow.At present, (5. lament) _______________,few students would recognise the idea that learning can be like that. But if educators invested a fraction of the energy on (6. stimulus) _______________ the students enjoyment of learning that they now spend in trying to transmit information, we could achieve much better results. Once students (7. motivate) _______________ is engaged, once they can be (8. power) _______________ to take control of their own learning and provided with clear (9. feed) _______________ on their efforts, then they are on their way to a lifetime of (10. propel)_______________ acquisition of knowledge. How did the discovery of gold in California affect the national debate over slavery? Check all that apply. It encouraged the expansion of slavery because slaves were needed to work in the mines. Californias admission threatened to upset the balance of free and slave states. The gold rush proved that slavery wasnt necessary for economic growth. Does anyone know this? Examine the steps used to solve the equation. 12. 5x 10. 2 = 3(2. 5x + 4. 2) - 6 1. 12. 5x 10. 2 = 7. 5x + 12. 6 6 2. 12. 5x 10. 2 = 7. 5x + 6. 6 3. 12. 5x = 7. 5x + 16. 8 4. 5x = 16. 8 5. X = 3. 36 analyze the steps to determine which properties or procedures were used to complete each step step 1: step 2: step 3: step 4: step 5:. The same quantity of each solute is added to water. Which solution will have the greatest number of H30+ ions at equilibrium?(1 point)O weak acidO strong acidO strong baseO weak base in front of which building in washington, d.c., did dr. king deliver his "i have a dream" speech? help me i'll brainiest if its right Can I get some help about a descriptive essay about Coronavirus What is the sum in 1 1/3+ 2 1/3 + 3 2/3 + 4 1/2 + 5 1/2 + 6 3/4 HELP I NEED THIS!! I'LL MARK BRAINLIESTlines AB and CD are straight lines. find x and y A cubical box with sides of length 0.368 m contains 1.980 moles of neon gas at a temperature of 298 K. What is the average rate (in atoms/s) at which neon atoms collide with one side of the container? The mass of a single neon atom is 3.35x10-26 kg. BETWEEN WHAT TWO INTEGERS IS THE FIRST ZERO? write the following ratios as fractions. 48 shoes 72 boots 64 hours and 2 days 147 rulers to 490 bags the ________ perspective of psychological disorders attributed symptoms of such ailments to forces that were beyond scientific understanding, such as black magic or evil spirits. What is the density of helium gas at 17c and 773 torr?. 2/7 convert it into percentage friends i only need solution i know the answer the one who answer i will mark them as brainleast a design repeated as a unit in a pattern is called a ________. help me solve this exercise g A pipe of negligible diameter is to be carried horizontally around a corner from a hallway 8 ft wide into a hallway 4 ft wide. What is the longest length that the pipe can be help with this plesseee