The β chains of HbA and HbS were treated with trypsin, and the sequence of the N-terminal tryptic peptides are as given below. Do these peptides separate from each other in an electric field if the pH is 7.0? Explain in detail the reasoning behind your answer and include your calculations for the charge of each peptide in your answer.

HbA: Val-His-Leu-Thr-Pro-Glu-Glu-Lys

HbS: Val-His-Leu-Thr-Pro-Val-Glu-Lys

Answers

Answer 1

Yes The two peptides will separate from each other in an electric field when the pH is 7.0

The two peptides wil drift/separate from each other when the pH is 7.0 because of the reaction of trypsin enzyme and also the difference in charge between HbA and Hbs

Considering charge of each peptide

Since The isoelectric point of normal Hba is 6.9 and Hbs possess two fewer negative charges per hemoglobin molecules

The Hbs is more hydrophobicity than HbA due to the substitution of glutamic acids of HbS by valine residue.

Considering Electrophoresis

At a lower pH level Hbs shows a slower moving band than Hba

while a higher pH level the HbS peptide is less negatively charged while the carboxyl group of HbA is more negatively charged and Hba moves faster as well as an acidic pH.

Hence we can conclude that the two peptides will separate from each other in an electric field when the pH is 7.0

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Related Questions

Guys how to make my hair tall and healthy

Answers

i dont have an answer, just wanted to add if anyone knows how to take care of super curly, untamable hair plz comment and give me some tips. i got chemo curls and they keep getting wilder each day. (yeah im ok, if you're wondering)
Ask your doctors for supplements about your hair. Buy growth oil supplements. Don’t towel dry. Use the right brush. Coconut oil is another great way to make your hair smooth and healthy.

Answer the following qs :
1- Mention three uses or benefits of fungi ?

Answers

Answer:

Humans use fungi for many purposes, including as food or in the preparation of food. Humans also use fungi for pest control. In addition, fungi can be used to produce citric acid, antibiotics, and human hormones. Fungi are model research organisms as well.

Explanation:

Humans use fungi for many purposes, including as food or in the preparation of food. Humans also use fungi for pest control. In addition, fungi can be used to produce citric acid, antibiotics, and human hormones. Fungi are model research organisms as well.

What structures are found in all cells? select two options

Answers

You’ll need to attach an image showing the choices or type them. But structures that are found in both plant and animal cells include the cell membrane, ribosomes, cytoplasm, and DNA.

thrips are insects that feed on rose

Answers

Answer:

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In which of these does a chemical change take place?
mixture
compound
solution
none of the above

Answers

Mixture because it’s reacting to a chemical change

4. How does the life of a sperm cell vary among the mosses, ferns, gymnosperms and angiosperms?

Answers

Answer:

They evolved on land to begin with from earlier now extinct groups groups so there was no need to adapt to life on land, other than to adapt to different terrestrial environmental pressures. Land can be everything from next to a river to a hot desert to rocks to Antarctica, and plants grow in all those places.

As well, there are thousands of species that are floating aquatics or submerged aquatics, including many ferns, and even some gymnosperms grow as marginals, and many species of angiosperms, mosses, ferns, and even some gmnosperms grow as epiphytes, or as parasites, or other ways.

Which of the following does NOT happen during the light-dependent reactions of
photosynthesis?
ATP is produced
Oxygen is produced
Glucose is produced
NADPH is produced

Answers

Answer:

Glucose

Explanation:

Only glucose is produced in the light independent stage of the reaction

Why is it necessary for there to be variation in population in order for evolution by natural selection to occur?

Answers

Answer:

There needs to be variations in population in order for natural selection to occur because the entire point of evolution by natural selection is only the best variations will survive. So if there were no variations then there would be no natural selection because the animal's survival rate would be the same no matter how many times they reproduce because there are no different variations being introduced into the species. However, if there are variations then the animal's survival rate could be impacted because of the variations, for example, white mice would be easier to find for predators on a dark surface, while a darker mice would be harder to find for predators on a dark surface, thus, allowing the darker mice to prevail as they have a higher survival rate and the species will slowly evolve into the darker mice. But, if there were no evolution, in this case, then no matter what happens, the white mice would not be able to evolve into a darker mice because there are no such thing as variation. That is why it is necessary for there to be variation in order for evolution by natural selection to occur.

Which of the following statements is FALSE?

Answers

Answer:

I'll wait for some possible answers...

Why are there not many vaccines for fungal & parasite disease as we have for viral and bacterial disease?

Answers

Answer:

it is very easy to kill the pathogen by vaccination but it is very difficult to detoxify the toxins produced in the host.

Explanation:

Hopefully this helped!

Many livestock are being grazed on public lands because the fees to graze on public lands are cheaper than the fees for private lands. If we aren't careful, what can this cause? A. owners moving their livestock TO private lands B. increased fees for private lands C. overgrazing on public lands D. overgrazing on private lands​

Answers

Answer:

I would say that this would cause "overgrazing on public lands."

Explanation:

When people see that the fees are cheaper, they would send their livestock there. It might be alot of livestock though

what is chloride shift ​

Answers

The chloride shift is an exchange of ions that takes place in our red blood cells in order to ensure that no build up of electric change takes place during gas exchange.

How can carbon can be stored for a short time in the natural cycle?

Answers

Answer:

The carbon cycle is nature's way of reusing carbon atoms, which travel from the atmosphere into organisms in the Earth and then back into the atmosphere over and over again. Most carbon is stored in rocks and sediments, while the rest is stored in the ocean, atmosphere, and living organisms.

A neuron that stimulates the gastrocnemius muscle receives signals from multiple areas of the brain. This is an example of

Answers

Answer:

Convergence

Explanation:

The hummingbird is more closely related to a lizard than it is to a dragonfly. Explain why two species that look similar are not necessarily closely related.

Answers

Some species look similar because they evolved in similar environments. Similar environments impose similar challenges, and traits improving survival are favoured. These organisms would not be closely related, however, because they evolved from different species and different regions. This is known as convergent evolution.

The __________________ is the tunnel that cuts through the external auditory meatus delivering sound to the ear drum or the _____________________.

Answers

Answer:

The auricle (pinna) is the visible portion of the outer ear. It collects sound waves and channels them into the ear canal (external auditory meatus), where the sound is amplified. The sound waves then travel toward a flexible, oval membrane at the end of the ear canal called the eardrum, or tympanic membrane.

Explanation:

How do genotyping help scientists to determine what plabt are

Answers

Answer:

We can determine the genotype of the plant by observing the phenotypes of the offsprings resulting from test cross.

which fungus does contain mycelium?​

Answers

Answer:

Mycelium is part of the fungi kingdom and is the network of threads, called hyphae, from which mushrooms grow. Not all mycelia fruit mushrooms, depending on the environmental conditions, but all mushrooms come from mycelia.

Explanation:

differences between stamen and pistil​

Answers

Stamen is composed of anther and filament. The pistil is composed of stigma, style, and ovary. ... The key difference between stamen and pistil is that stamen is the male reproductive organ which produces pollens of angiosperms while pistil is the female reproductive organ which produces ovules of angiosperms.

I hop this helps!

4. Explain the law of conservation of energy.

Answers

Law of conservation state that energy cannot be created or destroyed

Describe the structure and function of areolar connective tissue.

Answers

Answer:

Areolar Tissue is loose connective tissue that consists of a meshwork of collagen, elastic tissue, and reticular fibres - with many connective tissue cells in between the meshwork of fibres. The fibres that form the mesh structure of areolar tissue include: Collagen Fibres.

1. What is the major source of energy for the brain

Answers

Explanation:

glucose

The mammalian brain depends on glucose as its main source of energy. In the adult brain, neurons have the highest energy demand [1], requiring continuous delivery of glucose from blood.

hope its helpful to you #

The major source of energy for the brain is glucose. Metabolism of glucose provides energy to the brain.

What is the brain?

The brain is a body part that operates the various functions of the body. It is present in the head of the body. It is divided into two parts, the left brain, and the right brain.

Energy is something that is produced by the metabolism of food. Energy7 is required to carry out all the processes of the body. To walk, run, work, eat, everything requires energy.

The main source of the energy in animals and plants is glucose. The food we eat converts into energy by the process of respiration. The energy is transported into all parts of the body by blood circulation.

Thus, glucose is the major source of energy for the brain.

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Did you know that your bum can make three states of matter? Solid, Liquid, ad Gas

Answers

Answer:

Nope

Explanation:

Nope

Nope

Nope

Nope

Nope

Nope

Nope

Nope

Nope

Nope

Nope

Nope

Nope

Nope

Nope

Nope

Nope

Nope

Nope

Nope


4. What is a function of the nucleus of an animal cell?
A. It is the place where energy is produced.
It stores the genetic information, the DNA (chromosomes).
C. It defends the cell from infections.
D. It captures sunlight to produce food.
5. Select a statement that best completes the phrase below. In a plant

Answers

Answer:

A. It is the place where energy is produced.

It stores the genetic information, the DNA (chromosomes).

Explanation:

explain how biodiversity affects ecosystem services.

Answers

ecosystem services provided by biodiversity, such as nutrient cycling, carbon sequestration, pest regulation and pollination, sustain agricultural productivity. Climate change and other stresses have the potential to make major impacts on key functions, such as pollination and pest regulation services.

How does the carbon stored in the bodies of living organisms move into rocks?(1 point)

Carbon dioxide released through respiration dissolves in certain rocks, like limestone.

Living organisms decay, releasing carbon into the soil, and soil is compacted into rocks.

Living organisms decay and become fossils fuels, which eventually become rocks.

Carbon dioxide dissolves in ocean water and is slowly absorbed by rocks in the ocean.

Answers

In the atmosphere, carbon is stored in the form of gases, such as carbon dioxide. ... This carbon can then be ingested and stored in animals that eat the plants. When the animals die, they decompose, and their remains become sediment, trapping the stored carbon in layers that eventually turn into rock or minerals.

When animals die, their bodies degrade into the soil, encasing the carbon in layers that eventually transform into rock or minerals. hence option b is correct.

What is rock?

Stone including limestone and its minerals is created when sediment and shell layers are bonded together over time.

Gases like carbon dioxide are among the forms of carbon that are stored in the atmosphere. Animals that consume the plants can then absorb and store this carbon.

When animals die, their bodies degrade into silt, encasing the carbon in layers that eventually transform into rock or minerals. Some of this silt may eventually turn into fossil fuels like coal, oil, or natural gas, which when burned, release carbon back into the atmosphere.

Therefore, when an animal dies to decompose into the soil which becomes rocks through carbon from living organisms moves into rocks, hence option b is correct.

Learn more about rocks, here:

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What are examples of devices that use electromagnetic waves? Check all that apply.


-FM radios
-microwaves
=TV remote controls

Answers

Answer:

FM radios and TV remote controls.

Define bottleneck effect.


Koyi hai? ✌️​

Answers

Answer:

When disaster strikes, an ecosystem can change very quickly. When an event causes a drastic decrease in a population, it can cause a type of genetic drift called a bottleneck effect.

Hope this helps ~

Answer:

The bottleneck effect is an extreme example of genetic drift that happens when the size of a population is severely reduced. Events like natural disasters (earthquakes, floods, fires) can decimate a population, killing most individuals and leaving behind a small, random assortment of survivors.

Pls help....
I will brainliest you if you are good answer. ​

Answers

Answer:

1. 38.1 cm

2. 8 days

3. 20 blankets

4. 10 cups of detergent

5. 15 dry cloths

Explanation:

1. 15 x 2.54 = 38.1

2. 192/24 = 8

3. 12/3 = 4

   4 x 5 = 20

4. 20/4 = 5

   5 x 2 = 10

5. 10/2 = 5

   5 x 3 = 15

The ___________________ contains receptors for monitoring the position of the head.

Answers

Answer:

Vestibular Receptors

Explanation:

Located in the ear, only in the ear. When you are talking about position of other body parts this is called proprioceptors (sense positions of like arms, legs and other body parts).

Utricle and saccule, both in the vestibule;
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