To generate a knockin mouse, a scientist introduces to ES cells a construct with a mutant exon and within an adjacent intron, loxP sites flanking a neomycin resistance gene. What event will occur next?a. Crossing of heterozygous mice to a Cre-expressing strain.b. Homologous recombination between the mutant DNA and the corresponding gene in the mouse genome.c. Recombination between the loxP sites.d. Transplantation of cells into host blastocyst.

Answers

Answer 1

Answer:

b. Homologous recombination between the mutant DNA and the corresponding gene in the mouse genome

Explanation:

The Cre-lox recombination system is a versatile mammalian gene-editing tool used to produce site-specific recombination events in specific cell lines (e.g., embryonic stem or 'ES' cell lines) by facilitating the integration of a circular mol­ecule in genomic DNA. This system makes use of a Cre recombinase enzyme from bacteriophage P1 that has the ability to recombine specific sequences referred to as Lox sequences. By using this system, it is possible to generate knockout mice by crossing a strain of mice expressing Cre recombinase strain with a targeted loxP-flanked (floxed) mouse strain. The generation of a floxed mouse consists of 1-generation of a targeting construct containing homologous sequences with the mouse genome and loxP sites flanking the gene sequence of interest, and 2-homologous recombination of the targeting construct with the endogenous target gene sequence in the mouse genome.


Related Questions

Help I’ll give brainliest to whoever gets it right first

Answers

Answer:

solar eclipse

Explanation:

solar eclipse is the answer

Answer:

solar eclipse

Explanation:

when a solar eclipse occurs the sun is completely blocked in a solar eclipse because the moon passes between earth and the sun

1. For a(n) ecosystem to be healthy, it has to have lots of different kinds of plants and animals. 2. Name four types of ecosystems from those listed in the video. A. B. C. D. 3. Describe what Bill Nye was trying to demonstrate by removing some of the blocks in his tower of blocks. 4. All living things depend on _______________________________. 5. Name at least two species that have gone extinct. (four were shown) 6. The best way to wipe out a species is to _____________________________. 7. The largest ecosystem in the world is ______________, where 2/3 of all species live. 8. List five things you can do to increase biodiversity. A. B. C. D. E. 9. Of the 30,000,000 species on Earth, how many are lost each HOUR? 10. In conclusion, Bill Nye states that "As more and more species disappear, it's harder and harder to keep an ecosystem in balance because we don't know WHAT?

Answers

Answer:

4. food, shelter and water

5. Sabre-toothed Cat and Woolly Mammoth

6. food and water resources

7. World Ocean

9. 3 specie

Explanation:

All living things depend on their environment that supply food, shelter and water that is necessary for their survival. Sabre-toothed Cat and Woolly Mammoth are the two species that have gone extinct due to unfavourable environmental condition. The best way to wipe out a species is to remove food and water resources. The largest ecosystem in the world is World Ocean where two third of all species live. Of the 30,000,000 species on Earth, three species are lost each hour. In conclusion, Bill Nye states that "As more and more species disappear, it's harder and harder to keep an ecosystem in balance because every specie in the ecosystem balances other specie.

1. The ecosystem is defined as the relation of plants and animals, which depend on each other and work efficiently with the surrounding. For an ecosystem to be healthy, the diversity of the organisms are necessary and proper availability of resources.

2. The four types of the ecosystem are terrestrial, artificial, lentic, and lotic. Terrestrial refers to the ecosystem on land, artificial ecosystems can be human-made, lentic and lotic are the types of aquatic ecosystems.

3. In the video, Bill Nye was trying to demonstrate that everything depends on its building block and resources.

4. The biotic components of the ecosystem depend on food, shelter, and water.

5. Extinction refers to the complete elimination of an organism due to natural or human-made factors. Examples of extinct animals are the Wolly mammoth and Dinosaurs.

6. The best way to wipe out species is the change of ecosystem or the habitat in which the organism is thriving.

7. The largest ecosystem in the world is the ocean, where the majority of the species are found and some of them are yet to explore.

8. The biodiversity can be increased by increasing vegetation, diversifying the resources, making shelters available, and plenty of water.

9. Each hour approximately 3 species are lost. It can be due to natural calamity or human factors.

10. The preservation of the ecosystem is necessary, and the soon endangering of the species will lead to unbalance of the ecosystem, as species depend on each other.

To know more about ecosystem, refer to the following link:

https://brainly.com/question/1673533

Non living factors in an ecosystem are known as ______________________ factors.

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Answer: Abiotic

Explanation:

this is a real question, dont take it out of context GUYS. What is the white sticky stuff called that comes out of that plant that bugs die from if they eat? i also forget the name of the plant smh.

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Answer:

Mealybugs will commonly leave a white residue on a plant's leaves that resembles cotton. You will find this residue mostly on the stems and leaves. ... You may also find that the plant has a sticky residue on it. This is honeydew and is secreted by the mealybugs.

Explanation:

Answer: I d k

Explanation:

The average speed of an object is the

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Answer:-

B.) Distance Traveled by the object divided by the total time elapsed.

state the structure of dna

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DNA is a two-stranded molecule that appears twisted, giving it a unique shape referred to as the double helix. Each of the two strands is a long sequence of nucleotides or individual units made of: a phosphate molecule. a sugar molecule called deoxyribose, containing five carbons.

When pollution is added to a lotic water system, what happens to the oxygen level?

Answers

Answer:

in polluted systems, overgrowth of animals, plants and bacteria cause the oxygen to be used up quickly, sometimes causing fish to suffocate

hello please help i’ll give brainliest

Answers

Answer:

b

Explanation:

interaction between two different organisms living in close physical association, typically to the advantage of both.

HELP PLEASE 20 points

Answers

Aswer 10 because is ten 10

Answer:

Answer 10

Explanation:

11. All plant and animal life on Earth contains what element?
A)sulfur B)carbon C)silicon D)aluminum

Answers

Answer:

B.Carbon

I think sorry if it's wrong

Can someone please explain why food that has been kept at room temperature for a few hours is more likely to cause food poisoning than food that has been kept in the refrigerator?

Answers

Answer:

Because food kept at room temperature is somewhat exposed to air, and sometimes, the air can contain dangerous chemicals that were perhaps accidentally released, whereas food in the refrigerator doesn't apply to that problem.

Explanation:

Hope this helps, and please mark as brainlest! :)

Answer:

Because food that has been left out could be exposed to bacteria or get dried out, in the fridge it stays cooler and more fresh

ways of transmission of coronavirus from infected person to others?

Answers

Explanation:

We know that the disease is caused by the SARS-CoV-2 virus, which spreads between people in several different ways. The virus can spread from an infected person's mouth or nose in small liquid particles when they cough, sneeze, speak, sing or breathe.

6. Nitrogen Cycle - How can atmospheric Nitrogen become available for use by organisms?

Answers

Explanation:

Nitrates and ammonia resulting from nitrogen fixation are assimilated into the specific tissue compounds of algae and higher plants. Animals then ingest these algae and plants, converting them into their own body compounds. ... The action of these bacteria tends to deplete soil nitrates, forming free atmospheric nitrogen.

The atmospheric Nitrogen should become available for using the organism should be explained below.

Availability of atmospheric Nitrogen:

Here the nitrated and ammonia that should be lead from the fixation of the nitrogen that should be assimilated into the specific tissue compound with regard to algae and higher plants.

After this, the animal should be ingest these algae and plants now convert them into the compounds of the body.

Learn more about an organism here: https://brainly.com/question/17599295

no links and i know this isn't actually biology
3.
Which of the following characteristics of the Sun help life to exist on Earth?


1. The Sun’s gravity keeps the Earth in a consistent orbit.
2. The Sun’s radiation provides heat to the Earth.
3. The Sun’s light shines on half of the Earth at a time.
4. The Sun’s solar flares and storms shower the Earth with additional radiation.
5. The Sun’s spots cause cooler areas on the Sun’s surface.
6. The Sun’s position in relation to the Earth and Moon causes eclipses.

Characteristics 1, 2, and 3
Characteristics 2 and 3
Characteristics 2, 3, 4, and 5
Characteristics 3, 5, and 6

Answers

Answer:

characteristics

234

Explanation:

the people needs asun because the sun is shining and all tee time people needs the sun

Write a paragraph about air, land and water pollution.​

Answers

Answer:

AIR POLLUTION:

This tern is not something new, it has been existing since he time of industrial revolution where in big chimneys are pointed out in the air which releases harmful gases into the atmosphere. This phenomenon has been increasing since then as the industries have been developing simultaneously. Today, air pollutants have shown a great effect on plantations, health and life, decreasing the enjoyment of living, rising medical costs, causes global warming and also causes the destruction of the ozone layer.

One of the major causes of air pollution is the release of carbon di oxide into the atmosphere. This happens because of deforestation and fossil fuel burning. Sulfur dioxide is another main air polluter and is released into the atmosphere by the burning of sulfur containing compounds of fossil fuels. Sulfur dioxide is very harmful for the humans on high concentration and sulfur in the atmosphere is the main cause of acid rains. Chlorofluorocarbons also contribute to air pollution by reducing the ozone layer in the stratosphere. CFC’s are produced by the burning of plastic foam items, leaking refrigerator equipment and spray cans.

WATER POLLUTION:

Contamination of available natural water by industrial wastes and other waste products can be defined as water pollution. Factors causing water pollution can be categorized into two different groups; Point sources and Non- point sources.

Point sources are easy to identify and control. Some point sources of water pollution include waste products from factories, waste from sewage, waste from power plants , waste from underground coal mines and waste from oil wells. They are called point sources because these are the direct sources of water pollution and be reduced and monitoredNon- point sources are ambiguously defined and are much harder to control. The term non -point source encompasses a large range of sources like when rain and snow moves through the ground and picks up major pollutants and mover towards a major body of water, the runoff of fertilizers from farms animals and crop lands, air pollutants getting washed or deposited to earth, and storm water drained from streets parking lots and lawns.

LAND POLLUTION:

The contamination of and with different kinds of wastes is called land pollution. The major causes of land pollution are construction, domestic waste , industrial waste and agriculture.

The rising construction of building takes away the trees that takes away the homes of the animals living there. As there are more and more people inhabiting on our planet, the demand for food increases and the forests are cut down to make farm lands. In addition, Herbicides, pesticides, artificial fertilizers and animal manure are washed into the soil and pollute it. Tons of domestic waste is dumped everyday from homes offices etc. They can be recycled or burnt in incinerators. There are still more waste machinery products such as refrigerators and washing machines that are dumped in landfills simply because they cannot be recycled. Plastic factories, oil refineries, nuclear waste disposals, and large animal farms, all lead to land pollution.

WAYS TO STOP POLLUTION:

We can help to reduce global air pollution and climate change by few ways:

Driving a car that gets at least 35 mpg, walking, biking, and using public transportation, using CFL bulbs over incandescent bulbs, buying only energy efficient appliances, recycling newspaper, aluminum, and others, planting trees, avoid purchasing products that contains CFC’s, supporting much stricter clean air laws and enforcement of international treaties to reduce ozone depletion and slow global warming

pollution.

Describe three ways that the growth of suburban populations would have affected land and aquatic ecosystems around cities.

Answers

Answer: The three ways the growth of suburban populations( urbanisation) would affect the environment include:

--> Insufficient water availability,

--> Waste-disposal problems, and

--> High energy consumption.

Explanation:

The growth of suburban population is also called URBANISATION. This is defined as the rapid growth of an urban area which occurred due to the movement of individuals from rural areas into the urban area. There are different reasons why individuals migrate to urban areas these include:

--> for a better access to health care services

--> for more education opportunities

--> To combat the problems of unemployment and

--> for better participation in political and social activities.

The three ways the growth of suburban populations( urbanisation) would affect the environment include:

--> Insufficient water availability: the increase in the urban population creates a high demand for water use both for industrial and domestic uses.

--> Waste-disposal problems, and

--> High energy consumption.

Answer:

Deforestation, Water pollution, Introduction of invasive species

Explanation:

For ages, human beings have been altering the natural ecosystems and causing irreparable damages to nature. Development drove to an unmeasurable advance over new unpopulated areas.

Some of the principal human activities responsible for biodiversity loss are

• the uncontrolled use of unrenewable resources,

• overexploitation of renewable resources;

• land destruction for the mining industry, farming and cattle industry, buildings, and other infrastructures;

• the unplanned advance of cities, and opening of new roads to connect those cities.  

All of them involving

deforestation and fragmentation of natural landscapes; water, air, and soil pollution, species loss, and reduction of biodiversity. Introduction of invasive species.

And the growth of suburban populations is a frequent human activity causing an impact on nature. To mention a few ways in with this action might affect ecosystems, we can name the followings,

Deforestation:        

The growth of suburban populations is indefectibly related to deforestation.  New open areas are needed for buildings, roads, and service establishments, such as natural gas or water ducts, light, and others. There hardly exists planning about the cities´ growth and conscientious conservation of nature.

Water pollution:  

Water is severely polluted while constructing buildings and roads. The wastes of materials used for construction, oil coming from transport and machinery, human wastes, among other sources of contamination reach the rivers or lakes, polluting them and altering the natural equilibrium.

Introduction of invasive species:

It is very common to use ornamental vegetable species, proceeding from other areas or countries to arrange the gardens, streets, and parks. These species might become invasive and uncontrollable, causing severe damage in native endemic species by competing for resources. The whole ecosystem structure changes.

Deforestation, water pollution, and the introduction of invasive species are some of the causes of biodiveristy loss.

I need some ideas for experiments that show the process of evolution by natural selection. if this could be answered asap that would be super helpful!!

Answers

An example could be moths since certain moths in certain areas have a higher reproduction rate than others and have adapted camouflage to survive against predators

Please help with this question

Answers

Answer:

Foxes

Explanation:

The following statement is false. Please correct the statement to make it a true statement.

The retention time of a component can be used to tentatively identify the component, for this reason, this technique is considered a confirmatory lab test.

Answers

I’m going through a few minutes to see what I have done

Psychology that deals with police psychology, criminal profiling, and psychological
autopsies (case studies).

Answers

It’s called Criminal psychology, also referred to as criminological psychology, is the study of the views, thoughts, intentions, actions and reactions of criminals and all who participate in criminal behavior. Criminal psychology is related to the field of criminal anthropology.

help anyone need to get this done today???

Answers

simplest to complex: atoms, molecules, cells, tissue, organs, organ system, organism

I hope this helps!

Is the trait being tracked in the pedigree a dominant trait or a recessive trait?

Provide specific evidence from the pedigree (in terms of generation and individual’s numbers) and reasoning to support your claim.

Answers

Answer:

The trait being tracked is a recessive trait because less people have it.

7 people don't have it and 5 people do.

3. Which is not a true statement about the evidence to support
the origin of our universe?
A. Galaxies are expanding at an exponential rate in the
universe
B. The universe is continually heating up and gaining energy
C. Hydrogen makes up about 73%, and helium makes up
OUTPUT
about 25% of all the mass in the universe
STATION

Answers

Answer:

The answers that apply;

Researchers found cosmic microwave background radiation, which is a heat imprint left over from the big bang.

A redshift of light emitted by stars and galaxies indicates the universe is expanding

A primordial form of helium gas was found by the Endeavor space shuttle

Big Bang theory is a theory that endeavors to explain the origin of the universe. It stipulates that the universe exploded from a singularity and continues to expand today

Explanation:

Hope it helps:)

The statement 'the universe is continually heating up and gaining energy' is NOT true about the evidence to support the origin of our Universe.

The evidence states that the Universe is continually expanding, thereby celestial bodies are moving away from each other.

In this regard, it has been proposed that a faster expansion rate might be due to an unknown force that is pulling galaxies apart.

Moreover, it has also been suggested that as the Universe is expanding, its temperature slowly declines (i.e., the universe is becoming cooler) and loses energy.

In consequence, the Big Bang Theory about the origin of the Universe states that it cools down as it expands.

In conclusion, the statement 'the universe is continually heating up and gaining energy' is NOT true about the evidence to support the origin of our Universe.

Learn more in:

https://brainly.com/question/915856

Economic importance of monera and fungi essay

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Answer:

Bacteria are microorganisms and they are useful to us. Bacteria are economically important as these microorganisms are used by humans for many purposes. The beneficial uses of bacteria include the production of traditional foods such as yogurt, cheese, and vinegar. Microbes are also important in agriculture for the compost and fertilizer production. Bacteria are used in genetic engineering and genetic changes.

If there are only a few remaining members of an insect species living in one area, which would most likely cause this species to become extinct?

Answers

Answer:

introduction of new predators to the insects.

Answer:

the introduction of new predators to the insects’ environment

Explanation:

a cross between a white horse and a chesnut horse produce all light brown horses

Answers

Answer:

Incomplete dominance

Explanation:

- Eijiro <3

Answer:

incorrect.

Explanation:

there is a possibility. but in reality color does not effect the foal.

My Friesians grandfather was an Akhal teke that was gold. He gave birth to all black offspring as you can see in the attachment.


The real length of one villus is 0.8 mm
Calculate the image length if the villus is viewed at a magnification of 20
Use the equation:
size of image
magnification =
size of real object
[3 marks]

Answers

Answer: 16 mm

Explanation:

If I understood the given equation correctly, it could be rewritten as:

size of image = size of real object * magnification

Plug in the given values, and we get

size of image = 0.8 * 20 = 16 (mm)

Can anyone help? Please, Its very much needed. I can't move to the next part without this

Answers

Answer:

Biodiversity is defined as the variety of life forms existing on the planet and the number of species living within an area. The notion also covers the way diversity changes over time and moves from one location to another (“Facts on biodiversity,” n.d.). There is terrestrial and marine biodiversity that includes species living on Earth and in the sea. A number of species dwelling in a particular area helps to monitor the aspects of biodiversity. Maintaining biodiversity is important for people’s well-being since it plays a significant role in the contribution to human health and provision of raw materials. However, currently people often face the loss of biodiversity. Today this process is happening at a much faster pace than it did 50 years ago (“Facts on biodiversity,” n.d.). As a matter of fact, around 90 percent of all the fish on the planet was exploited within the last 50 years, a quarter of bird kinds were killed, and 70 percent of forests was destroyed. In addition, extinction threatens 18 percent of all mammals, 5 percent of the fish, and 8 percent of plants. Thus, it is possible to assume that the loss of biodiversity on Earth, which takes place nowadays, is directly linked to anthropological factors, including overhunting, overfishing, consumerism, and agricultural practices.

Explanation:

this weather system map shows 4 locations, W, X, Y, and Z across the united states. Which of the following statements is true based on the map?

Answers

B because it’s in the middle of the cold front and warm front

¿Qué opción justifica la inversión económica para realizar las investigaciones espaciales?

Answers

Answer:

Incluso una fracción del dinero gastado anualmente en exploración espacial podría salvar a millones de personas en países afectados por la pobreza y mejorar las condiciones de vida de las generaciones futuras. Los cimientos del mundo en el que vivimos se basan en gran medida en la ciencia y, de hecho, es vital ampliar nuestro conocimiento del universo.

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Please help me! It isnt hard im just not sure how to do it . After the death of Solomon, a. Israel was destroyed by the Phoenicians. b. the judges again ruled Israel. c. his kingdom split in two: the northern kingdom of Israel and the southern kingdom of Judah. d. the prophet Isaiah seized the throne. Trevor's new mastercard credit card has an introductory 6 month 0% APR. His average daily balance for the first month was $2,350. What is his finance charge for the first month 80 POINTSWhich group of words from the passage best helps the reader understand the meaning of the word extinction? In what way does this cartoon illustrate the concept of cause and effect? Solve the equation1 (x 2) {(x -3) (x 1)} = 0 OLEASE HELPWhich number produces a rational number when added to 1/2 A. 5.38516480.... c. 10D. 0.22 cheryl wants to buy a bicycle that costs 160$. if she saves 12$ each week, what is the fewest number of weeks she must save in order to buy the bicycle? please help me. f(x) = In(x)^6 find f'(x) What is Resume called ? *a. essayb. letterc. bio-datad. novel 9) An object with a height of 18 cm is placed in front of a converging lens. The image has aheight of 9.0 cm.a) What is the magnification of the lens?b) If the focal length of the lens is 6.0 cm, how far in front of the lens is the object?c) Where does the image appear? A heron is perched in a tree 50 feet above sea level. Directly below the heron, a pelican is flying 17 feet above sea level. Directly below the birds is a trout, swimming 23 feet below sea level.Select all the true statements.A The difference in height between the pelican and the heron is -33 feet.The difference in height between the pelican and the heron is -33 feet.B The difference in height between the pelican and the heron is 33 feet.The difference in height between the pelican and the heron is 33 feet.C The distance between the heights of the pelican and heron is -33 feet.The distance between the heights of the pelican and heron is -33 feet.D The difference in height between the pelican and the trout is -40 feet.The difference in height between the pelican and the trout is -40 feet.E The difference in height between the pelican and the trout is 40 feet.The difference in height between the pelican and the trout is 40 feet.F The distance between the heights of the pelican and the trout is 40 feet. Which expression is equivalent to 1/3b - 7 summary of on his blindness by john milton PLEASE HELPPP!!!!write the correct form of tener and ser which expert from the odyssey best shows odysseus Demonstrating the epic hero traits of strength and leader ship 7. How can you use nonverbal communication to express an idea to someone? gavin walks at a rate of 3 miles per hour.at this rate how far will walk in 4hours Which of the following is an example of improper form when walking at a fast pace? (5 points)Focusing straight aheadKeeping the chin upMoving hips forwardTensing hands into a fist "two less than the product of seven and a number?"