What was the purpose of the cell lysis solution?

Answers

Answer 1
Cell lysis is used to break open cells to avoid shear forces that would denature or degrade sensitive proteins and DNA. Cell lysis is used in western and Southern blotting to analyze specific proteins, lipids, nucleic acids, reporter assays, immunoassays, and protein purification. Hope this helps:)
Answer 2

Cell lysis is used to break open cells to avoid shear forces that would denature or degrade sensitive proteins and DNA.

What is Cell lysis?

Lysis refers to the breaking down of the cell, often by viral, enzymic, or osmotic mechanisms that compromise its integrity. A fluid containing the contents of lysed cells is called a "lysate".

Mammalian Protein Extraction Reagent is a novel nonionic detergent designed for efficient whole-cell protein extraction from cultured mammalian cells. It gently and rapidly dissolves cell membranes at low concentrations without denaturing proteins.

Protein Extraction Reagent is a proprietary formulation of nonionic detergents that is optimized for the most efficient extraction of soluble proteins from bacterial cells.

Therefore, Cell lysis is used to break open cells to avoid shear forces that would denature or degrade sensitive proteins and DNA.

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Related Questions

a wheel with a rope or belt around it helps person lift or move something up or down

Answers

i think a wheel with a rope will lift someone up but a wheel???

Label this plant cell organelle.

Answers

Answer:

chloroplast

Explanation:

Chlorapst is the correct answer

How does climate change impact Earth?

Answers

Answer:

Effects on weather. Global warming leads to an increase in extreme weather events such as heat waves, droughts, cyclones, blizzards and rainstorms. Such events will continue to occur more often and with greater intensity.

Which Feature do prokaryotic and Eukaryotic cells share?

Answers

Answer:

Both prokaryotic and eukaryotic cells have structures in common. All cells have a plasma membrane, ribosomes, cytoplasm, and DNA

Explanation:

Hope this helped!!!

Answer:

they both have a plasma membrane

they both have DNA

they both have ribosomes and both go through protein synthesis

they both have cytoplasm

Brown eyes are d o m i n a n t over blue eyes in humans. A brown-eyed male marries a blue-eyed female. They have 10 children, all brown-eyed. Can we be certain that the man is h o m o z y g o u s d o m i n a n t? If the 11th child has brown eyes, will that prove the father's genotype is? Explain your reasoning, or show a Punnett Square. (word is spaced out because of brainly's guidelines.)

Answers

Answer:

No.

Explanation:

Based on the punnets square (assuming that eye color is a one gene trait in this question) There is a 75% chance they would have a child with brown eyes and a 25% chance that the child would have blue if the father is heterozygous. Theoretically the father could be heterozygous and still have 11 brown eyed kids. This just means that the Dad always passed down his dominant gene.

What is meant by natural variation in living things? *
Different species have different attributes.
Different individuals within a species have variation because they may express
different alleles for the same traits.
All the possible trait variations of a single population.
The number of organisms a given area will support.

Answers

Answer:

Different individuals within a species have variation because they may express

different alleles for the same traits

( please help) What type of mutation is shown in the diagram above?
A. Inversion
B. Insertion
C. Deletion
D. Point

Answers

Answer:

D.

Explanation:

I think it may be point because only one section is changing.

Will give brainliest

Answers

Answer:

B. 40%

Explanation:

I hope this helps you. If you are looking for something good to listen to, listen to Find me by Forest Blakk. Its very sad and calming at the same time.

Is sickle cell anemia a terminal or a non terminal disorder?

Answers

Answer:

terminal

hope this helps

have a good day :)

Explanation:

Answer:

terminal

Explanation:

its extremely deadly, usually you dont live past infancy but in some cases if they prolong it long enough you can live to your teens

HELP I NEED HELP ASAP HELP I NEED HELP ASAP HELP I NEED HELP ASAP HELP I NEED HELP ASAP HELP I NEED HELP ASAP

Answers

Answer:

an increase in water pollution

excessive rain

Describe what the scientific method is and why it is important to the scientific community.

Answers

Answer:

The scientific method is a process for gathering data and processing information. It provides well-defined steps to standardize how scientific knowledge is gathered through a logical, rational problem-solving method. Scientific knowledge is advanced through a process known as the scientific method.

Explanation:

I hope that helped..

name two strategies of human body defence system​

Answers

Answer:

You have external defense systems, which include your skin, tears, mucus, saliva, stomach acid, cilia (small hairs) and helpful bacteria. The skin, nails and hair form the integumentary system, which stops dirt and pathogens from entering the body.

Where is the immune system

Answers

The immune system is the bodies defense against invaders and is located in all body tissues with concentration in the mucosal surfaces and skin.

Answer:Primary lymphoid organs: These organs include the bone marrow and the thymus. They create special immune system cells called lymphocytes. Secondary lymphoid organs: These organs include the lymph nodes, the spleen, the tonsils and certain tissue in various mucous membrane layers in the body

Explanation: BRAINLIEST pls

How many kingdoms are there in the domain Eukarya? O A. 1 O B.2 O c. 4 O D. 3​

Answers

Answer:

There are 4 kingdoms

There are currently four kingdoms in the domain named Eukarya. The correct option is c.

What is domain?

In biological classification, a domain is a taxonomic category above the kingdom level. Bacteria, Archaea, and Eukarya are the three domains of life.

These domains are defined by differences in cell structure, biochemistry, and other characteristics of the organisms that inhabit them.

Bacteria and Archaea are both prokaryotic domains, whereas Eukarya is a eukaryotic organism domain that includes animals, plants, fungi, and protists.

In biology, a kingdom is a taxonomic rank made up of smaller groups known as phyla (or divisions, in plants).

Historically, the highest taxonomic rank, or the most general taxon used in classifying organisms, is kingdom.

Animalia, Plantae, Fungi, and Protista are the current kingdoms in the domain Eukarya.

Thus, the correct option is c.

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Draw a food chain that includes the ants, fungal gardens, mold, and bacteria. Label the drawings and add arrows to show how the four organisms interact.

Answers

Answer:

Symbiosis and decomposers.

Explanation:

The ants feed on both plants and insects so we can say that ants are omnivorous. They make a symbiotic relationship of mutualism with fungi such as mold which helps the ants to breakdown the cellulose of plants whereas plants provide habitat to fungi. The bacteria feeds on the dead body of ants and release nutrients by performing the function of decomposition so bacteria acts as decomposer so in that way these four organisms interact with each other.

PLEase help asap I will give BRAINLIEST/ CROWN!!! for the correct answer! LINKS AND SPAMS WILL BE REPORTED

Biologists measure the length of a tagged sea turtle. They learn that the turtle is 2.3 cm longer than the last time it was measured. Where did the sea turtle get the nutrients it needed to grow?

from the jellyfish it ate
from sunlight it absorbed
from the oxygen it breathed
from nutrients it absorbed from the water

Answers

Answer:

from the jellyfish it ate

Explanation:

it is right answer

PLEASE HELP ASAP!!! WHOEVER IS CORRECT WILL BE GIVEN BRAINLIEST!!! LINKS AND SPAMS WILL BE REPORTED!!


Sawgrass takes in light energy during photosynthesis. What happens to most of this energy?
1 point
It is stored as chemical energy
It disappears as it used up by the plant.
It is transferred to organisms that eat the grass
It is used to help the sawgrass grow and reproduce

Answers

Answer:

It is used to help the sawgrass grow and reproduce

Explanation:

Tell me if I'm right or wrong pls

Answer:

It is used to help the Sawgrass grow and reproduce.

Explanation:

Although it being transferred to organisms that eat the grass is a very plausible answer, the energy being used to help the Sawgrass itself makes more sense because the Sawgrass' needs come first. I could be wrong with this one, but that's what I think. Hope this helps!

Protein synthesis actually begins in the nucleus when ___ transcribe ___ ?

Answers

When DNA transcribes RNA

.The millionaire, Mr. Big, has just died. He has left behind a wife, daughter and a large inheritance. The news of his death has brought forth 2 men who claim to be the long lost son of Mr. & Mrs. Big. Before Mr. & Mrs. Big were married, they had an illegitimate child and had placed him up for adoption. They had tried to find him after they became wealthy but had no luck in locating him. A DNA sample was taken from Mrs. Big, the Big daughter and the two men who claim to be the long lost son. Which, if any, of the men are telling the truth? Analyze the DNA sample below to answer the question. Explain how you determine the correct answer.

Answers

Answer:

Explanation:

use this it might help

As per the question, DNA analysis can be used to determine the biological relationship between individuals, so if the DNA of the two men claiming to be Mr. Big's son matches that of Mrs. Big and Mr. Big's daughter, it would be strong evidence that they are indeed Mr. Big's biological children.

What is DNA analysis?

DNA analysis is a scientific technique that is commonly used to identify biological relationships between individuals. It works by comparing the genetic markers or sequences of the DNA samples collected from the individuals in question. DNA is the genetic material that contains the genetic instructions for the development and function of all living organisms. In the case of the Big family, if one or both of the men claiming to be Mr. Big's son have a DNA sequence that matches the genetic markers of Mrs. Big and the Big daughter, it would be a strong indication that he is indeed the biological son of Mr. Big. The DNA analysis would be able to provide information about the probability of the biological relationship based on the number of matching genetic markers or sequences.

Hence, DNA analysis can be used to determine the biological relationship between individuals, so if the DNA of the two men claiming to be Mr. Big's son matches that of Mrs. Big and Mr. Big's daughter, it would be strong evidence that they are indeed Mr. Big's biological children.

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HELP PLEASE I WILL GIVE YOU THE BRAINLIEST

Answers

Answer:

rain, steep, agriculture, ?, keep

Explanation:

Six examples of vectors​

Answers

Answer:

Mosquitoes West Nile Virus St. Louis Encephalitis Western

Answer:

Plasmid

Phage

Cosmids

Bacterial Artificial Chromosomes

Yeast Artificial Chromosomes

Human Artificial Chromosome

Explanation:

Hope this helps!

:)

True or false? Transcription produces a strand of mRNA that is based off the DNA
A. True

B. False

Answers

True cuz DNA strand that is transcribed for a given mRNA is termed the template strand!
Hope that helps!

Answer:

A. True

Explanation:

Transcription produces a strand of mRNA that is based off the DNA = True

Does anyone know how to do this?

Answers

Answer:

gang

Explanation:

How are all paths that have a displacement of zero similar?
30 POINTS!
PLS NO SAMPLE

Answers

Answer:

Displacement is identified as the net movement of a thing with detail to its initial location. One may journey out of his city and come back. ... This is true for all trails that have zero displacement that they all go back to their initial position.

Explanation:

Displacement is identified as the net movement of a thing with detail to its initial location. One may journey out of his city and come back.

Explanation:

The other guys response just want to say that its correct

Explica el impacto del desarrollo de las técnicas moleculares en los sistemas de clasificación de los organismos.

Answers

Answer:

Las técnicas moleculares, especialmente aquellas basadas en la manipulación del material genético, permitieron automatizar el proceso de identificación y clasificación de especies

Explanation:

Las técnicas moleculares usadas para la clasificación de organismos son aquellas principalmente relacionadas a la manipulación de su material genético. En especial, las técnicas de extracción de ADN, amplificación mediante la reacción en cadena de la polimerasa (PCR) y posterior secuenciación han posibilitado automatizar el proceso de clasificación de organismos. Los marcadores moleculares de ADN basados en secuencias génicas evolutivamente conservadas tales como, por ejemplo, la secuencia del gen Citocromo Oxidasa I (COI) en animales, son ampliamente usados para automatizar este proceso de identificación y clasificación de nuevas especies.

A female firefly responds to signals flashed by a male firefly. What are these fireflies participating in?
A courtship


B hibernation


C migration


D defense

Answers

Answer: I think it’s A

Explanation: the male is trying to attract the female for mating

The oldest crust on the ocean floor is found in the center of the mid-ocean

True or False

Answers

Answer:

that's all I can get

is that enough

earth is divided into large geographic regions or which are based on differences in climate​

Answers

The Answer is Biomes

So im about done with my essay how does it look so far


Vaping can expose you to nicotine, and that can cause cravings and other symptoms of addiction. It can also expose your lungs to toxic metals like nickels, lead, chromium, tin, and aluminum. Nicotine changes the way your brain works so as a teen your brain is still developing and that can make it where you're more vulnerable to nicotine addiction. You inhale 3 chemicals when you vape. When addicted to vaping you can get popcorn lung and the effects are, coughing, wheezing, chest pain, shortness of breath. It also can affect your memory. 1. Diacetyl: This food additive, used to make the flavor, will damage small passageways in the lungs. It's also a compound found in foods and additives used for dairy flavoring. It's found in brown flavors, such as caramel, butterscotch, and other sweeteners. 2. Formaldehyde: This can cause lung disease and contribute to heart disease. It's a colorless, strong-smelling gas used in making building materials and many household products. It is also used in pressed-wood products, like particle board, plywood, and fiber board. 3. Acrolein: It's most often used as a weed killer, this can also damage lungs. It's a colorless liquid with a piercing, acrid smell. The smell of burnt fat. Vaping has some effects like tobacco as in picking at your face so much it leaves scars. It also gives you mood swings. Vaping can lead to other addictions in life. You will feel more anxious than usual. It can also lead to cancer, diabetes, eye problems, sickness, gum infection and disease, heart problems, impotence, infertility, lung disease, pregnancy complications. If you're athletic it would be harder to do your sport because you would lose breath faster and you wouldn't be able to do it for a long time. So if you play sports and you vape you will have to quit your sport or quit vaping. It also makes you depressed (more) because of the brain damage you're causing .

Answers

Maybe get rid of “So” in the end and it seems good

Please help!
Some species of plants maintain a symbiotic relationship with bacteria that live in nodules on the plant's roots. Which is a likely function of such bacteria that would benefit the plant?
A) phosphorus fixation
B ) nitrogen fixation
C) aerobic respiration
D) photosynthesis

Answers

Nitrogen fixation
Answer:
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