You are attempting to carry out a transposon mutagenesis experiment using the pRL27 system. The Donor strain you use was giving to you by a lab mate who has been culturing it continuously for several months. When you set up your control plates, you find that the Donor strain grows poorly on Luria DAP Agar. Your transformation efficiency is very poor for this attempt at mutageneis. What likely happened to the Donor strain

Answers

Answer 1

The most likely happened to the donor strain is that the transposon is active in the donor resulting in the gradual accumulation of mutations. The accumulation of mutations is promoted by the insertion of the transposon.

A donor strain is a bacterial strain that contains a mobilizable fragment of DNA called plasmid.

These strains (donor strains) exploit the transference properties of a conjugative plasmid to modify a particular recipient strain.

Transposons, also known as jumping genes, are mobile genetic elements that alter the genome of an organism by jumping and thus inserting them into genetic sequences (i.e., into protein-coding sequences).

In this case, the presence of a transposon in the donor strain affected the expression of many genes required for the donor strain in order to grow into Luria DAP Agar.

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Related Questions

What evidence of climate change on continents supports the theory of continental drift?

signs that glaciers existed in South Africa
similar coal fields on different continents
change in atmospheric temperature over time
same animal fossils found on different continents

Answers

Answer:

A

Explanation:

Edge2022

The evidence of climate change on continents that supports the theory of continental drift is similar coal fields on different continents. Hence option B is correct.

What is continental drift theory?

The theory of continental drift suggests that the Earth's continents were once joined together in a single landmass called Pangaea, which began to break apart around 200 million years ago.

This idea was first proposed by German scientist Alfred Wegener in the early 20th century, who noticed the striking similarities between the coastlines of Africa and South America.

Coal is formed from the remains of plants that lived millions of years ago, and its distribution can provide clues about how continents were once connected.

Coal fields found on different continents that match in age and composition indicate that the continents were once joined together in a larger landmass.

This supports the theory of continental drift, which states that the continents were once part of a single supercontinent that later broke apart and drifted into their present positions.

This evidence suggests that coal-forming swamps once existed in locations that are now separated by oceans and that these locations were once connected.

Hence option B similar coal fields on different continents is correct.

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Unranus gas a giant, storm that rotates similar to a hurricane?
True
False

Answers

Answer:

True

Explanation:

PLEASE HELP ME IM TIMED!

Answers

Answer:

All living things are composed of single cells or multiple cells

Explanation:

The cell theory states that all biological organisms are composed of cells; cells are the unit of life and all life come from preexisting life. The cell theory is so established today that it forms one of the unifying principles of biology.

Pls help I'll mark brainliest

Answers

Answer:

1. Carbon dioxide

2. Water

3. Glucose

4. Oxygen

5. reactants

6. products

7. carbon dioxide

8. oxygen

9. glucose

10. water

11. chloroplast

12. chlorophyll

Explanation:

Answer:

Explanation:

glucose + oxygen        carbon dioxide + water

5. reactants                      6. products

why is the cell membrane sometimes called the fluid mosaic model?

Answers

Answer:

Because it has many types of molecules that float along the lipids due to the many types of molecules that make up the cell membrane

Explanation:


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What is Cost ???​

Answers

Answer:

An amount that has to be paid or spent to buy or obtain something is called cost.

Answer:

brainliest me

Explanation:

thx for the points

What are the primary elements of the nervous system responsible for analyzing and transmitting information

Answers

Answer:

Neurons

Explanation:

Neurons are the fundamental units of the brain and nervous system, the cells responsible for receiving sensory input from the external world, for sending motor commands to our muscles, and for transforming and relaying the electrical signals at every step in between. Hope this helps :)

Does a candy bar, piece of beef, butter, or bread turn up with the color blue/black on a Lugol’s (starch) test?

Answers

Answer:

bread passes the test for starch

When a nerve impulse reaches the end of an axon of a neuromuscular junction, calcium flows ______ the synaptic knob.

Answers

Answer: flows INTO the synaptic knob.

When a nerve impulse reaches the end of an axon of a neuromuscular junction, calcium flows into the synaptic knob.

In a neuromuscular junction, there are three parts. They are the presynaptic terminal or synaptic knob, the synaptic cleft and the postsynaptic terminal. The calcium ions will work in the synaptic knob when the nerve impulse arrives.

Once that the nerve impulse reaches the end of the axon, it will affect the presynaptic terminal. In there, it causes the opening of voltage-gated calcium channels. As a result:

Calcium flows into the presynaptic terminal.The calcium makes the vesicles (with neurotransmitters) descend and fuse with the terminal membrane to release the neurotransmitters to the synaptic cleft.

In conclusion, the nerve impulse causes the opening of voltage-gated calcium channels, and calcium flows into the synaptic knob.

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Does surface area increase permeability of membrane

Answers

Answer:

The magnitude of the concentration gradient depends on the membrane permeability. The concentration gradient changes the available surface area. It is suggested that a high-permeability compound is absorbed mainly from the top of the villi, while a low-permeability

Explanation:


Humans can use starch as an energy source but are unable to use cellulose for energy. Which
of the following best explains the difference in how these molecules are processed in the body?
Humans can chew starch but cannot chew cellulose.
Humans dissolve starch in blood plasma but cannot dissolve cellulose
Humans make an enzyme to digest starch but cannot digest cellulose
Human muscle cells can absorb starch but cannot absorb cellulose

Answers

Answer:

human make enzymes to digest starch

If you help me I'll give you a brain list

Answers

I answered the first image in another question for you, though I noticed that the heart to body question was never answered (or at least properly answered).

The blood leaves our heart through the aortic valve, and travels into the aorta which ultimately leads to the body.

Hope this helps!

HOW
TO
PROJECT: BUILDING DNA
The structure of DNA resembles a twisted ladder called a double helix. For this project, you will build a DNA molecule by
placing its basic parts together like pieces of a puzzle.

Answers

The structure of DNA resembles a twisted ladder because both strands are linked by hydrogen bonds.

What is DNA?

Deoxyribonucleic acid (DNA) is a double helix composed of two long chains of nucleotides linked by hydrogen bonds.

In DNA there exist only four different types of nucleotides (i.e., Guanine, Cytosine, Thymine and Adenine).

In conclusion, the structure of DNA resembles a twisted ladder because both strands are linked by hydrogen bonds.

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10. What are the products of aerobic respiration? نقطة Ůاحدة a. Carbon dioxide and Water b. Glucose and Oxygen c. Glucose and Water d. Carbon Dioxide and Oxygen.

Answers

Answer:

a) Carbon dioxide and water

Explanation:

The reaction is called aerobic respiration, and it produces energy which transfers to the cells. Aerobic respiration makes two waste products:carbon dioxide and water.

Which adaptation is most likely to help a plant obtain water in a dry environment?

A. A waxy stem coating

B. Many sharp spines

C. A brightly colored flower

D. Many shallow roots

Please help me answer!!

Answers

Answer:

spines lose less water in hot enviroments and help protect against against animals so B.

The best advantage of sexual reproduction is?
It is more efficient than asexual reproduction
It is safer than asexual reproduction
It results in identical offspring
It increase genetic diversity through meiosis, crossing over, and random assortment of chromosomes

Answers

Answer:

it increase the genetic diversity through meiosis , crossing over and random assortment of chromosome

Explanation:

There are two main processes during sexual reproduction: meiosis, involving the halving of the number of chromosomes, and fertilization, involving the fusion of two gametes and the restoration of the original number of chromosomes.

while asexual reproduction doesn't form any reproduction that involves neither meiosis nor fusion of gametes

4.

Which process produces the greatest amount of ATP per glucose molecule metabolized?
respiration
c. fermentation
b. digestion
d. photosynthesis
A. Respiration

Answers

Explanation:

Explanation: The electron transport chain generates the most ATP out of all three major phases of cellular respiration. Glycolysis produces a net of 2 ATP per molecule of glucose.

Answer:

a

Explanation:

Identify the type of blood vessel shown below. How does its structure match its function?

Answers

Answer:

Can you also send the diagram

A body was found at 12:22am on April 19. The temperature of the
body when found was 94 degrees. What was the approximate time of death?

Answers

343883822828827382829

Special proteins that bind to enzymes during the cell cycle are called
a.CDK
b.Growth promoting factor
c.Cyclin
d.checkpoint

Answers

Answer:

cyclins and cyclin-dependent kinases (Cdks), are

Explanation:

I'm sorry if I'm wrong! :{

Cell process and transport toturial

Answers

Sorry Brainly can not provide this answer

Which of the following statements defines ovulation?

Ovulation is when an immature ovum develops into a mature ovum.
*Ovulation is the release of a mature ovum, which occurs once each month.
Ovulation is the production of an ovum that occurs each month.
Ovulation is the monthly discharge of the uterine lining.

Answers

Answer:

b. Ovulation is the release of a mature ovum, which occurs once each month.

Explanation:is correct. post protected

Ovulation is the release of a mature ovum, which occurs once each month. The correct option is B.

What is ovulation?

The process of an egg being released from the ovary is called ovulation. The egg is released and travels down the fallopian tube, where it remains for 12 to 24 hours before being fertilised.

Although ovulation is necessary for conception, more than simply an egg is required. For instance, there needs to be a clear path to the egg. A blocked fallopian tube prevents pregnancy from happening.

Around the time of ovulation, you could notice that your cervical mucus is wetter, clearer, and more slippery. After ovulation, there is a slight increase in body temperature that you might be able to measure with a thermometer.

Thus, the correct option is B.

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how is the structure of a leaf optimized for photosynthesis

Answers

Answer:

- The upper epidermis of the leaf is transparent, allowing light to enter

- They have a large surface area to volume ratio, allowing more light to enter

The upper epidermis of the leaf is transparent, allowing light to enter and they have a large surface area to volume ratio, allowing more light to enter.

What is responsible for photosynthesis to occur?

The mesophyll cell is responsible for photosynthesis occurs. The majority of the chloroplasts and the primary site of photosynthesis are located in the palisade layer. The area of storage and gas exchange is the airy, spongy layer. The stomata control the water and carbon dioxide equilibrium.

For gaseous exchange and photosynthesis, leaves are suited. Since they have a wide surface area and stomata, which enable carbon dioxide to enter the leaf and oxygen to exit, they are well-equipped for photosynthesis.

Chloroplasts, which is known as the house the chlorophyll in plants, are where photosynthesis occurs. The thylakoid membrane, a third inner membrane that creates lengthy folds inside the chloroplast and is bordered by a double membrane, is present inside chloroplasts.

Therefore, The upper epidermis of the leaf is transparent, allowing light to enter and they have a large surface area to volume ratio, allowing more light to enter.

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How does a frog survive in water as well as on land?

Answers

An amphibian can live both on land and in water. ... Instead of drinking water, frogs soak the moisture into their body through their skin. Frogs breathe through their nostrils while also absorbing about half the air they need through their skin.

Hope this helps :) - sorry if I am wrong - if I am right can I get brainliest please??

NEED URGENT HELP!

A. What are three things that can create microclimates?

B. What characteristics of these things help create a microclimate?

Answers

Topography (height), large bodies of water and urban areas can create microclimates

in a hot place, if theres a mountain there, the top of the mountain can be cold

cement in an urban area can absorb a lot of heat during the day and then release it at night

valleys can have cold air

What is the function of mitosis?
a) To produce four unique cells for sexual reproduction
b) To change one type of cell to another type of cell,
c) To heal a cell that has been damaged,
d) To produce two identical daughter cells to sustain an organism

Answers

Answer:

c

Explanation:

sorry if it's wrong but yea

Answer:

d

Explanation:

Receptors that are especially important for helping a person maintain balance are located in the (A) gyrus cinguli (B) inner ear (C) tendons (D) ossicles (E) ligaments

Answers

The receptors that aid in allowing a person to maintain balance are located within the inner ear.

The human ability to walk upright without falling, and a wide variety of other tasks that we perform as all thanks to our ability to maintain balance. This ability is given to us by structures located deep within the inner ear.

The inner ear is located deep within the lateral side of the head. This ear contains 3 semicircular canals or ducts, which are tiny structures filled with liquid. Each of these canals has an expansion at its end known as the ampulla, which holds within it the very small cellular balance receptors called crista.

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What is the importance of microtubules in cell division?(1 point)

They provide a pathway for organelles to move into the newly formed cell.


They reform the nucleus in the divided cell.


They organize and pull the chromosomes apart.
.

They start the process of cytokinesis.

Answers

Microtubules are very important in a number of cellular processes. They are involved in maintaining the structure of the cell and, together with microfilaments and intermediate filaments, they form the cytoskeleton. They also make up the internal structure of cilia and flagella.

Answer:

C) They organize and pull the chromosomes apart.

Explanation:

in the comments!

Hope this helps for all the other students looking for the answer!




Depletion time describes the length of time it takes to use up

Answers

Answer:

80% of a mineral reserve

Explanation:

i got 100% on this unit

2) What are the primary functions of all the Organic Compounds/Macromolecules?

Answers

Answer:

enter in formation of the body

Explanation:

provide cells with energystore energyhelp from cell membrance
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