You are doing an experiment in which you are using an in vitro translation system derived from rabbit reticulocytes, called a rabbit reticulocyte lysate, to translate mRNA in a test tube. The rabbit reticulocyte lysate contains ribosomes, translation initiation and elongation factors, tRNAs, aminoacyl tRNA synthetases, release factors, amino acids, and ATP. The lysate does not contain any mRNA, so you must add the mRNA you would like to translate to the system. A. When you analyze the proteins made from your lysate, you find that they are all only two amino acids long. At first, you think that there must be a mutation to a stop codon in the position of the third codon, so you do another experiment with a different mRNA. Again, all the newly synthesized proteins are only two amino acids long, so you conclude that there is a problem with some component in your lysate.

Describe ONE possible problem in the translation system (lysate) that would result in the limitation of newly synthesized peptides to two amino acids in length.

Answers

Answer 1

Answer:

Problems with transfection efficiency or absence of essential mRNA modifications (capping and poly-A tail)

Explanation:

Translation is the process of protein synthesis from RNA in which the genetic information encoded in a messenger RNA (mRNA) molecule is translated into a protein sequence composed of amino acids. Translation has three steps: initiation, elongation, and termination. During translation, different elements are required to initiate, continue and complete the process, which include essential molecules such as amino acids, mRNA, tRNAs, ribosomes, energy-carrying molecules (i.e., ATP, GTP), initiation factors (i.e., eIF1A, eIF3, etc) and elongation factors (eEF-2, EIF5A). For example, humans can't synthesize nine essential amino acids (i.e., threonine, tryptophan, phenylalanine, valine, methionine, leucine, histidine, isoleucine, lysine), thereby it is imperative to include these amino acids to synthesize proteins composed of them. On the other hand, it is also fundamental to take into account that the efficiency of transfection of the molecules required during translation, as well as the absence of modifications in the mature mRNA sequence, may alter the process in vitro.


Related Questions

Is your prediction supported by the membrane potential chart?

Answers

Answer:

The membrane potential of a resting neuron is primarily determined by the movement of K+start text, K, end text, start superscript, plus, end superscript ions across the membrane. ... Zero voltage across the membrane, as measured by a voltmeter with one electrode inside and one electrode outside the cell.

Answer:

Yes, it is. The chart shows that the initial charge of the neuron is negative. When the neuron is stimulated, sodium ions enter the cell. So, the voltage inside the cell changes to positive. When potassium ions move outward, the voltage decreases until it reaches its previous state.

Explanation:

Which of these lymphoid organs destroys bacteria before it can breach the intestinal wall and generates "memory" lymphocytes for long-term memory?

Answers

Answer:

Peyers patches

Explanation:

Peyers patches are group of lymphoid follicles found in the ileum of the small intestine.

This part of lymphatic system form part of immune system that help to control bacteria population in the small intestine and prevent the growth of pathogenic bacteria in the small intestines. It plays an important role in immune surveillance where it recognises pathogenic bacteria and destroy the bacteria.

It is a lymphoid organs destroys bacteria before it can breach the intestinal wall and generates "memory" lymphocytes for long-term memory because it form the part of the immune system which help to fight infection or foreign substances in the body, also by generating lymphocytes which is a white blood cells of the immune system which generate long term memory of foreign pathogens and destroying it.

interact to produce a trait in which neither dominant gene can produce its effect in the presence of the other.
O Multiple alleles
Chromosomes
O Carriers
Sex linked genes

Answers

The answer is multiple alleles

Answer:

A

Explanation:

Knowing the epidemiology and causative agent of Legionaries disease what questions would you ask of the victims or of their surviving relatives?

Answers

Answer:

Answered below

Explanation:

Legionnaires disease is a type of lung inflammatory disease or pneumonia, which is caused by infection from the bacteria Legionella bacterium.

Legionella bacterium can be spread by inhalation from water or soil. People with weakened immune systems are most susceptible as are smokers. Untreated legionnaires disease can progress to a fatality.

Some of the questions to ask of a victim or of surviving relatives include;

1) Any visits to swimming pools?

2) Source of drinking water?

3)Any visits to fountains?

4) Contacts with victims and their personal belongings?

5)Is any relative or victim immunocompromised? Do they have diseases that might lower their immune response?

6) Are they smokers?

14. Which of the following may produce more than one functional protein
from an mRNA transcript?
a. chromatin condensation c. epigenetics
h transcrintional regulation d. alternative mRNA processing

Answers

Answer:

D. Alternative mRNA processing.

Explanation:

why the swimmer push the water backward​

Answers

to go propele themselves across the water

Answer:

[tex]\boxed{\mathrm{view \: explanation}}[/tex]

Explanation:

Swimmers push water backwards to propel forwards. In other words, swimmers push their hands and legs against water to move forward. This is stated in the third law of motion, for every action, there is an equal and opposite reaction.

How many more phases occur during meiosis than during mitosis?
none
two
four
six

Answers

Answer:

there are 4 more phases that occur during meiosis than mitosis

Answer:

The answer is.....

Explanation:

C. Four

Fill in the blanks.
1. The phylum contains most common animals you are familiar with su
as eagles, dogs, foxes, and frogs.
2. While the earth is home to many members of this phylum, earthworms are
a member of
3. The most successful phylum on earth is which contains insects,
spiders, and crustaceans.
4. An animal that molts it exoskeleton is called a(n).
5. are cells that have the potential to become any type of cell; they hav
not yet received their genetic programming.
6. All members of the animal kingdom are multicellular, heterotrophic and
7. A starfish is a bilaterian deuterostome with symmetrical adults.
8. Complete the chart by describing what kinds of organs or tissues each type
germ layer becomes:
Moi

Answers

Answer:

1. chordata

2. nematoda

3. arthropoda

4. ecdysozoan

5. stem Cells

6. eukaryotic

7. radically

Chart answer:

Endoderm = Lungs, liver, pancreas, lining of gut organs

Mesoderm = Bone, muscle, heart, circulatory system

Ectoderm - Brain, nervous system, skin

Explanation:

Answer:

1. chordata

2. nematoda

3. arthropoda

4. ecdysozoan

5. stem Cells

6. eukaryotic

7. radially

8. Endoderm… Mesoderm becomes… Ectoderm becomes…

Lungs, liver, pancreas, lining of gut organs

Bone, muscle, heart, circulatory system

Brain, nervous system, skin

Explanation:

Please help me come up with an answer for this question thankyou so much! Compare and contrast the processes of cellular respiration and photosynthesis. Think of as many similarities and differences as you can (things to consider: inputs and outputs, the role of energy, the types of organisms that do each process, the location within cells)

Answers

Explanation:

The end result is glucose, with oxygen being the waste product. The two processes are similar in that they both produce energy, albeit in two different forms. They are different in that photosynthesis assembles the glucose molecule, while cellular respiration takes it apart.


what is the importance of autotrophic organisms for other organisms?

Answers

Answer:

the importance of autotrophic organisms with respect to energy flow and nutrient cycling in ecosystems. Primary producers usel solar or chemical energy to manufacture their own food and they use this energy through cellular respiration and growth and reproduction--energy used for NPP.

A pharmaceutical company is working on a drug to slow the spread of Ebola. Which of the following should be the company's focus?

a. Sanitizing the drinking water
b. Protecting the food supply
c. Person-to-person contact
d. Killing the insect vector

Answers

Answer:

C

Explanation:

Although Ebola isn't transferred by the air and through touch. Bodily fluids like sweat, saliva, urine, breastmilk, semen, and vomit all transmit the disease, if a person gets it in a cut or is directly introduced by it (sexual intercourse.)

How did Dinosaurs diminished from earth?​

Answers

Answer:

Hey there

It is believed that the dinoursars diminished from earth through a massive meteorite which weight 2 trillion tons which hit the earth causing maximum heat and it killed all the dinoursars

Explanation:

Have a nice day

it’s been a while since i’ve learned this, but dinosaurs got diminished my a huge meteorite and on the other side where it didn’t hit, there was toxic gasses that stayed for years

Research each insect, then compare and contrast the life cycles. You will need to write your observations and thoughts down. Be sure to include and describe each stage of development. Your observations, descriptions, and thoughts should be at least 150 words in length.



Incomplete Metamorphosis Complete Metamorphosis
grasshopper butterfly
dragonfly ladybug
cicada housefly
cockroach honeybee

Answers

Answer:

Larva and pupa is present in complete metamorphosis while nymph is present instead of larva and pupa in incomplete metamorphosis.

Explanation:

Complete metamorphosis has four stages i. e. egg, larva, pupa, and adult while the incomplete metamorphosis has three stages i. e. egg, nymph, and adult. Grasshopper, dragonfly, cicada and cockroach have incomplete metamorphosis while ladybug , housefly, butterfly  and honeybee have complete metamorphosis. Both type of insects lay eggs and after hatching a baby insect comes out called larva. After sometime this larva transforms from immature to mature, this stage is known as pupa. After pupa, the insect converts into an adult which is ready for reproduction. Nymph is a stage that comes in place of larva and pupa in incomplete metamorphosis. in this stage, the insect is small in size and lack of wings but in adult stage the wings appear.

Please help!!!!! science, please explain in sentence cause yes i don’t know where to find these answers !

Answers

Answer:

1. DNA replication.

2. rRNA - is called the ribosomal RNA which serve as structural components of protein-making structures known as ribosomes.

mRNA - is called the messenger RNA which serve as temporary copies of the information found in DNA.

tRNA - is called transfer RNA, that ferry amino acids to the ribosome to be assembled.

3. Protein synthesis.

Explanation:

Hope it helps.

Larry recalls his first memory of riding a red tricycle at the age of 4. Larry’s memory is: True False

Answers

Answer:

True

Explanation:

False memory refers to remembering an event differently from the way it happened or remembering events that never happened at all.

Larry's memory is a true memory because it doesn't sound like something that could be made up. Also, most children ride tricycles around the age of 4 or even younger.

Hope that helps.

Saliva is released by __________ and it acts on _______.

Answers

Answer:

Salivary glands, food

Explanation:

Salivary glands release saliva, and it acts on the food and turns into a soft pulpy mass called bolus. Bolus is the simplier form of food which helps in digestion of complex food.

Hope this helps. Mark me as the Brainleist if you want...

Answer:

salivary glands, food

Explanation:

Saliva is released by salivary glands and it acts on food.

Salivary glands produce saliva. The saliva acts on food and creates a soft lump called bolus during the chewing process.

Which of the following could be discovered by studying the gross anatomy of a cadaver?
The function of cells within the body
The types of cells within each organ
The function of the body's organs and systems
The structure of the body's organs and systems

Answers

Answer:

The structure of the body's organs and systems

Explanation:

In medicine, a cadaver is a dead human body dissected in order to study its anatomical structure. It is employed by medical students and other medical practitioners to study the structure of the human body.

Anatomy is a branch of science that has to do with the study of the structure of living organisms. Relating anatomy to cadaver, these dead bodies are dissected in order to view and study the structure of the different compartments of the body e.g organs, systems etc.

Below are two chemical equations. One is for cellular respiration and one is for photosynthesis. Correctly place the reactants and products in the right location to complete both equations.
Photosynthesis: H20 +H20------------->C6H12O6 +O2
+solar energy CO2
Cellular Respiration:O2+C6H1206 -------->CO2 + H2O
+02

Answers

Answer:

Photosynthesis: 6CO₂ + 6H₂O + solar energy ----> C₆H₁₂O₆ + 6O₂

Cellular Respiration: C₆H₁₂O₆ + 6O₂ ----> 6CO₂ + 6H₂O + ATP

Hope that helps.

The correct equation for cellular respiration and one for photosynthesis are:

Photosynthesis: 6CO₂ + 6H₂O + solar energy ----> C₆H₁₂O₆ + 6O₂

Cellular Respiration: C₆H₁₂O₆ + 6O₂ ----> 6CO₂ + 6H₂O + ATP

What is cellular respiration?

Cellular respiration is a process of converting food into energy that can be used in many processes. It happens when oxygen is combined with food material and releases carbon dioxide as a waste product.

The correct reaction of photosynthesis is when carbon dioxide and water are combined with sunlight to form glucose and oxygen.

The correct reaction of cellular respiration is when glucose and oxygen react and release carbon dioxide and water and energy,

Thus, the correct reactions are:

Photosynthesis: 6CO₂ + 6H₂O + solar energy ----> C₆H₁₂O₆ + 6O₂

Cellular Respiration: C₆H₁₂O₆ + 6O₂ ----> 6CO₂ + 6H₂O + ATP

Learn more about cellular respiration, here:

https://brainly.com/question/12671790

#SPJ2

Why were the advent of metagenomics, and the application of molecular techniques such as single-cell and next-generation sequencing, important for defining a microbial relationship

Answers

Answer:

Based on novel approaches, it has been seen that symbiotic associations prevails between a host and one to thousands of more microbes, which challenges the previous meaning of symbiotic association as one that takes place between the host and a single microbe. It was considered previously that symbiotic association’s takes place only between the pair of single microbe and host.  

However, newer approaches has revealed that such kind of association can take place between one microbe and many other hosts. A symbiotic association can take place between microbe-microbe, plants-plants, microbe-plants, and others. However, symbiotic association has never been found to take place between a bacteria and animal host. The symbiotic associations are always not parasitic.  

How can you guarantee a high expression of your protein in any expression vector?

Answers

Answer:

protein expression refers to the way in which protein are synthesized, modified and regulated in living organisms.

Explanation:

In the protein research,the term can apply either the object of study or the laboratory techniques required to manufacture proteins.

Which of the following techniques involves hybridizing a cDNA sample to a chip containing thousands of single-stranded DNA sequences, allowing one to study the expression of thousands of genes simultaneously?
A) PCR
B) Southern blot
C) FISH
D) Agarose gel electrophoresis
E) DNA microarray

Answers

Answer: Option E.

DNA micro array.

Explanation:

DNA microarray is a technique that involves hybridizing cDNA into a chip that contains thousands of single stranded DNA sequences. This technique enables one to study expression of thousands of genes at the same time.looks for extra (duplicated) or missing (deleted) chromosomal segments, sometimes called copy number variants (CNVs).

DNA microarrays are microscope slides which are printed with thousands of tiny spots in specific different positions, and each of the spots contain a known DNA sequence or gene.

primary use of DNA microarrays is transcriptional profiling.

The basic principle behind the DNA microarray is “ nucleic acid hybridization.

Which interaction is an example of commensalism?
O A. Insects helping plants reproduce while eating the nectar in their
flowers
O B. Bacteria living inside a human's stomach and making the person ill
C. Birds building nests in the branches of trees
O D. Mosquitoes eating the blood of cows and passing on a disease
SUB

Answers

C. The birds benefit from the shelter of the tree branches without harming the trees

Answer:

C

Explanation:

Commensalism is where one organism benefits while the other is neither harmed nor benefited.

A - Both parties benefit

B - Bacteria benefit, the human is harmed

C - Birds benefit, the tree neither

D - Mosquitoes benefit, cow is harmed

During which period did humans first appear on Earth? Quaternary Neogene Paleogene Cenozoic

Answers

Answer:

the answer is actually Quaternary, please double check your answer before giving them out to people

we dont want them to get a bad grade

Explanation:

i took the test

E2020

2. If a nucleus has 8 chromosomes, how many chromosomes will be present after Telophase I? 3. What advantage does crossing-over have in reproduction? 6. Compare and contrast spermatogenesis and oogenesis (where do they occur, how many gametes are formed, when do they develop, etc):

Answers

Answer:

Telophase I: 8 chromosomes composed of two sister chromatids  

Crossing over refers to the mechanism of recombination, this process being characterized in that increases the genetic variation

Spermatogenesis refers to the mechanism of formation of male gametes (sperms), while oogenesis is the process of formation of female gametes (oocytes)

Explanation:

Telophase I is the final step in meiosis I. During telophase I, the nuclear membrane is formed around the decondensed set of chromosomes and the nucleoli reappear.  

Spermatogenesis refers to the process by which haploid male germ cells (sperms) are generated within the seminiferous tubules in the testis, while oogenesis refers to the process of differentiation of the female egg cells (ova or oocytes) in the ovaries. Spermatogenesis is known to produce small and motile spermatic cells, while oogenesis is known to produce bigger non-motile oocytes.

What is the relationship between coronary artery disease and a heart attack?

Answers

Answer:

C. Coronary artery disease can reduce blood flow to the heart and cause heart attacks

Explanation:

im pretty sure

Which of the following practices that are needed to run a nuclear power plant has a negative impact on both air and water

Answers

Answer:

The options are

a.cooling of the power plant

b.mining of uranium

c.storage of radioactive waste

d.monitoring the reactor

The answer is b.mining of uranium

Explanation:

Uranium is known as a radioactive element which is used as a fuel to power a nuclear energy plant. Mining of uranium could cause it to mix with air and water which results in contamination.

When contamination occurs and it gets into the body of humans or animals by inhalation or ingestion it poses a high risk of cancer and kidney damage.

The uterine cycle describes the cyclic changes of thickening and degeneration that the endometrium goes through in a month. What is the order of events in one uterine cycle

Answers

Answer:

During the uterine month there are different phases through which the uterus passes, these phases are regulated by hormones and are responsible for producing the cycle necessary for fertilization.

Phase where menstruation occurs: This phase only happens if the woman was not fertilized and did not develop the diploid cell together with a sperm, since not being fertilized, all the uterine preparation that had been planned in the body for fertilization will be released as that we know "menstruation", in this phase estrogens and progesterone are low. The inner walls of the unfertilized uterus are released.

Follicular phase, in the follicular phase the ovaries prepare to release an egg and estrogen begins to rise. (From the first day of the period until ovulation)

Proliferative phase, in the proliferative phase, new vessels proliferate and the outermost layer of the uterus prepares itself for possible fertilization, is where spiral arterioles can begin to form again in the external cut of the myometrium.

Ovulation, here is where the mature ovum is called Graff's follicle, at this time estrogen reaches its peak and then descends.

Luteal phase, in the luteal phase the production of the luteal body is generated, at this stage progesterone takes center stage, and it is the range between ovulation and menstruation (if not fertilized)

Last phase, secret phase, in this phase there are two possible ends, if the woman is fertilized, the egg cell implants and begins the development of the embryo and if it is not fertilized, the entire external cut of the myometrium is prepared to be secreted.

Explanation:

A very important fact to clarify is that women are born with a quantity of ovules that at the end of this uterine cycle ceases to exist, this process is what we know as menopause.

That is to say that women have a quantity of ovules that will one day run out, and the body releases them from the menarche or the first menstruation, generating that in each released ovule a uterine cycle is completed, the day they end the woman will have reached menopause and would have no chance of being fertilized or completing the uterine cycle.

superficial layer of the endometrium is shed

basal layer of endometrium grows, forms gland and blood vessels

enriched endometrial blood supply

endometrial glands secrete nutrients into uterus

whats 20x20? thank you

Answers

Answer:

400 yayyyyyy

Explanation:

yvhjghkkkkhhh

Answer:400

Explanation:

20

x20

_____

00

+400

--------

400

Hemoglobin is the blood protein responsible for the transport of oxygen. Carbon monoxide disturbs oxygen transport by

Answers

Answer:

Answered below

Explanation:

Hemoglobin is found in the red blood cells and is responsible for the transportation of oxygen from the lungs to the various body cells.

Oxygen binds to hemoglobin because it has a high affinity for it. This aids its transportation. When it gets to the cells it unbinds and get transported into the cell for use in energy production.

Carbon monoxide is an odourless, dangerous gas which has more affinity for hemoglobin than oxygen. Therefore when carbon monoxide is inhaled, it quickly binds to hemoglobin, thereby displacing oxygen. It binds to hemoglobin for a longer period and due to this, the body does not get any oxygen and cells begin to die.

Symptoms of carbon monoxide poisoning include dizziness, headaches, fatigue and coma.

Explain why chemogenetic based methods of regulating cellular signaling result in poor temporal control of biochemical pathways relative to optogenetic based methods.

Answers

Answer:

They use different detecting methods to assess neuronal circuits

Explanation:

Chemogenetics is a technique widely used in neuroscience research to explore signaling interactions by means of genetically modified receptors capable of interacting with small molecules. Chemogenetics was first used to determine the function of the chalcone isomerase gene by inducing mutations that altered its substrate specificity. On the other hand, optogenetics is a bioluminescence-driven genetic technique used to control genetically modified neurons that express light-gated ion channel genes. This technique is also used to monitor neuronal networks. In consequence, optogenetic and chemogenetic techniques have recently been combined in order to analyze neuronal circuits, it by analyzing the same actuator molecule.

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Help plz down below with the question Fill in the table using this function rule.y=-6x-1 Help PLZ!!! Which statement accurately represents the arrangement of electrons in Bohrs atomic model? -Electrons move around the nucleus in fixed orbits of equal levels of energy. -Electrons move around the nucleus in fixed orbits of increasing levels of energy. -Electrons move randomly in the relatively large space surrounding the nucleus. -Electrons vibrate in fixed locations around the nucleus. The legal document identifying the rights and obligations of both the bondholders and the issuer is called the bond ______. This document describes the number of bonds authorized, their par value, and the contract interest rate. agreement document indenture article Grace starts with 100 milligrams of a radioactive substance. The amount of the substance decreases by 14 each week for a number of weeks, w. She writes the expression 100(14)w to find the amount of radioactive substance remaining after w weeks. Ryan starts with 1 milligram of a radioactive substance. The amount of the substance decreases by 40% each week for a number of weeks, w. He writes the expression (1 0.4)w to find the amount of radioactive substance remaining after w weeks. Use the drop-down menus to explain what each part of Graces and Ryans expressions mean. DeLong Corporation was organized on January 1, 2017. It is authorized to issue 14,500 shares of 8%, $100 par value preferred stock, and 450,000 shares of no-par common stock with a stated value of $3 per share. The following stock transactions were completed during the first year.Jan. 10 Issued 84,500 shares of common stock for cash at $6 per share.Mar. 1 Issued 5,150 shares of preferred stock for cash at $110 per share.Apr. 1 Issued 23,500 shares of common stock for land. The asking price of the land was $91,000. The fair value of the land was $81,500.May 1 Issued 84,000 shares of common stock for cash at $5.00 per share.Aug. 1 Issued 10,000 shares of common stock to attorneys in payment of their bill of $39,500 for services performed in helping the company organize.Sept.1 Issued 11,500 shares of common stock for cash at $7 per share.Nov. 1 Issued 2,000 shares of preferred stock for cash at $111 per share.Journalize the transactions. The mean one-way commute to work in Chowchilla is 7 minutes. The standard deviation is 2.4 minutes, and the population is normally distributed. What is the probability of randomly selecting one commute time and finding that: a). P (x < 2 mins) _____________________________ b). P (2 < x < 11 mins) _____________________________ c). P (x < 11 mins) ________________________________ d). P (2 < x < 5 mins) _______________________________ e). P (x > 5 mins) What was brinkmanship and what was the result of the policy in the 1950s? Identify how many distinct classes there were in Rome What is the image of the point by (-5,3) under a 270 rotation about the point (-7,-3) An inverse variation includes the point (4,17). Which point would also belong in this inverse variation? what is transmission of heat? Graph a line that contains the point (-7,-4)and has a slope of - 2/3 A concert starts at 7:45pm and ends at 1:35 am. How long was the concert? what is your understanding about politics?? n a group discussion about Frida Kahlo, Selma asks whether it is fair to say that Frida Kahlo is one of the most important female painters of the twentieth century. By asking this question, she has become the leader of the discussion. has provided her personal point of view. has become the moderator of the discussion. has provided an important fact about the topic. The graph of h(x) is a translation of f (x) = RootIndex 3 StartRoot x EndRoot. On a coordinate plane, a cube root function goes through (negative 3, negative 1), has an inflection point at (negative 2, 0), and goes through (negative 1, 1). Which equation represents h(x)? Ben has $500 in his savings account and the bank pays an interest rate of 10 percent a year. The inflation rate is 6 percent a year. The government taxes the interest that Ben earns on his deposit at 20 percent. Calculate the nominal after-tax interest rate and the real after-tax interest rate that Ben earns. Ann Chovies, owner of the Perfect Pasta Pizza Parlor, uses 20 pounds of pepperoni each day in preparing pizzas. Order costs for pepperoni are $10.00 per order, and carrying costs are $0.04 per pound per day. Lead time for each order is 3 days, and the pepperoni itself costs $3.00 per pound. If she were to order 80 pounds of pepperoni at a time, what would be the average invent Question 4. In the graph, lines f and g intersect at P(6,6). What is the area, in square units, of the shaded region? * E. 15 F. 21 G. 27 H. 30