You are trying to program E. coli bacteria to generate N-acylethanolamide, lipids that have been found to help suppress appetite. In order to do this, you need to transfer the gene for the enzyme N-acyltransferase from the plant Arabidopsis Thaliana into the E.coli bacteria.

The transgene, which codes for ***********, will also need to have a**********
for RNA polymerase and a ********** that would fluoresce to let you know the insertion was successful. You would also need a********** , a small circular piece of bacterial DNA.
Then you would hunt for a *********** that would cut the plasmid once and also cut the transgene out from its host.

FILL IN THE **********

Answers

Answer 1

Answer:

The transgene, which codes for N-acylethanolamide, will also need to have a transcription initiation site for RNA polymerase and a selectable marker (reporter gene) that would fluoresce to let you know the insertion was successful. You would also need a plasmid, a small circular piece of bacterial DNA. Then you would hunt for restriction enzymes that would cut the plasmid once and also cut the transgene out from its host.

Explanation:

A transgene can be defiend as a gene (in this case, the N-acylethanolamide gene) which has been transferred by using genetic engineering techniques from one organism to another. The RNA polymerase is an enzyme required to copy a DNA sequence (e.g., a gene) into an RNA sequence, during transcription. RNA polymerases bind to specific DNA sequences called transcription initiation sites on the DNA template, and this binding triggers the initiation of the transcription. A plasmid is a small circular DNA molecule separated from chromosomal DNA which is capable of replicating independently of the chromosomal DNA. Plasmids are widely used in genetic engineering to clone/transfer/manipulate transgenes. During transgenesis, the plasmid is cut with a specific restriction enzyme in order to ligate the transgene of interest into the plasmid. Finally, the green fluorescent protein (GFP) gene is a selectable marker (i.e., a reporter gene) that indicates which cells were successfully modified because its expression can be detected without any substrates.


Related Questions

Strong contrasts in texture have what effect on a monochromatic design?

Answers

Answer:

It prevents monotony

Explanation:

Strong contrasts in texture have a primary effect on monochromatic design by ensuring it’s more eye catching and not too plain.

Contrasts is a term used to describe the display of an objects dissimilar sides. A monochromatic light is known to have just one color and the strong contrasting texture however makes it possible for it to have different color differences thereby preventing monotony.

Organisms can pass on genetic information through asexual or sexual reproduction. Which of the following statements best describes how asexual reproduction differs
from sexual reproduction?
During asexual reproduction, offspring receive DNA from several parents rather than just one parent.
During asexual reproduction, offspring receive DNA as individual atoms rather than genes.
During asexual reproduction, offspring receive DNA from one parent rather than two.
During asexual reproduction, a parent cell divides by meiosis rather than mitosis.

Answers


During asexual reproduction, offspring receive DNA from one parent rather than two.

Which is not a difference between a population showing exponential growth and a population showing logistic growth?

A. Food limits
B. Disease limits
C. Carrying capacity
D. Mutation rate

Answers

Answer:

D

Explanation:

Answer:

D

Explanation:

Hello brainliest pls?

You have identified a new, recessively inherited trait in dogs: floppy ears. To determine if the gene for Floppy Ears is X-linked or not, you should:

Answers

Answer:

Cross a non-floppy-eared male to a floppy-eared female and observe if males offspring have the floppy ear trait

Explanation:

Mammals (e.g. dogs) have two sex chromosomes: X and Y. In the XY sex-determination system, the female gamete (i.e., the ovum) contributes to an X chromosome, while the male gamete (i.e., the sperm) contributes either an X chromosome or a Y chromosome: normal females are XX and normal males are XY. In consequence, a male always inherits its X chromosome from the mother and its Y chromosome from the father. In this case, the presence of the floppy ear trait in all males F1 offspring obtained by crossing a non-floppy-eared male to a floppy-eared female indicates that this trait is linked to the X chromosome because males inherit the floppy ear trait from the mother.

1. Catalytic converters reduce which of the following pairs of pollutants in car exhaust? a.)Carbon dioxide and ozone
b.)Carbon monoxide and hydrocarbons
c.)Carbon dioxide and particulates
d.) Lead and mercury
e.)Methane and particulates​

Answers

Answer: B

Explanation: By most estimates, catalytic converters fitted inside the exhaust pipe of a gasoline-operated car convert over 90% of hydrocarbons (HC), carbon monoxide (CO) and nitrogen oxides (NOx) from the engine into less harmful carbon dioxide (CO2), nitrogen and water vapour.

The catalytic converter used in the exhaust of the cars reduces carbon mono oxide and hydrocarbons.

What is a catalytic converter?

The catalytic converters are the devices used in the exhaust of the vehicles run on petrol and diesel.

They convert the harmful toxic gases into the less harmful pollutants by a redox reaction.

By most estimates, catalytic converters fitted inside the exhaust pipe of a gasoline-operated car convert over 90% of hydrocarbons (HC).

carbon monoxide (CO) and nitrogen oxides (NOx) from the engine into less harmful carbon dioxide (CO2), nitrogen, and water vapor.

Thus the catalytic converter used in the exhaust of the cars reduces carbon mono oxide and hydrocarbons.

To know more about Catalytic converters follow

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What genetic and other changes might have caused these cells to escape normal cell cycle regulation these cells are hela cancer cells

Answers

Answer:

Cancer cells such as Hela cells escape to normal cell cycle by mutations that lead to the misexpression and/or overexpression of regulatory molecules (e.g., cyclins and cyclin-dependent kinases) and faulty checkpoint control of the cell cycle  

Explanation:

The term cell cycle refers to the mechanism of DNA replication and cell division, which involves a series of sequential steps. The control of the cell cycle depends on 1-cyclins and cyclin-dependent kinases (CDKs) that regulate the cell cycle by a cascade of protein phosphorylations and 2- a group of checkpoint controls that monitor the completion of critical cellular events. HeLa is an immortalized cell line derived from cervical cancer cells. These cells (HeLa) contain 76 to 80 total chromosomes (instead of the 46 chromosomes that contain normal-diploid body cells), some of which are heavily mutated. These mutations can affect CDKs that control the cell cycle as well as checkpoint-control mechanisms (for example, those resulting from DNA damage), thereby cells do not enter programmed cell death (apoptosis) and proliferate uncontrollably.

¿ que sustancias produce energía por combustión ?

Answers

Answer:

En la combustión completa se produce: vapor de agua, dióxido de carbono y energía calórica. En cambio, si la combustión es incompleta, se produce monóxido de carbono.

how many chromosomes in the dna?​

Answers

Should be 23 I think?

Please help. I need this asap 30 points please

Answers

Answer:

Hydrosphere- 2nd example. (Rain falls from the...)

Lithosphere- 3rd Example (Volcanoes erupt...)

Biosphere- 1st example. (Plants absorb carbon...)

Explanation:

:) good luck!

There is you're answer :D

Explanation:

The Hydrosphere is Rain that falls from the cloud to replenish the plants.

The Lithosphere is Volcanos and related to the crust of the earth.

The Biosphere is planted absorbing carbon dioxide during photosynthesis.

PLEASE HELP, 20 POINTS! An example of meiotic cell division is shown below. In the diagram, genetic material is shaded according to the parent from which it was inherited.

(diagram shown below)

Based on the diagram, which processes contributed to the genetic diversity of the daughter cells?

A.
cytokinesis and differentiation
B.
differentiation and independent assortment
C.
independent assortment and crossing over
D.
crossing over and cytokinesis

Answers

Answer:

d, crossing over and cytokenesis

Answer:

It would be c. independent assortment and crossing over

Explanation:

(No. of chromosome pairs) X 2=
(No. of chromosomes)


Please help this is science

Answers

Answer:

.ejejejeejejejjejeeje

1c) If the amino acid sequences in the proteins of 2 organisms are similar, why will their DNA also be similar?

Answers

Answer and Explanation:

Because the DNA sequence determines a protein's amino acid sequence, a gene shared by two closely related organisms should have similar, or even identical, amino acid sequences. That's because closely related species most likely diverged from one another fairly recently in the evolutionary span. Thus, they haven't had as much time to accumulate random mutations in their genetic codes.

A vaccine used against an infectious disease may contain??

Answers

Answer:

antigens

Explanation:

a vaccine may contain that

names of five natural things from the word grid given below
AS T R PILM
o w LS PR P 0
RO AM
MS O LILIN
ART OTWAN
MIT
THE
IN
0
01
OTA NIMA
NA 0 OM
Q
RIO
R
LISI N​

Answers

Answer:

AIR

SEA

ANIMAL

PLANT

MONTH

Explanation:

These are the least I could get

Please help if you don't know don't answer. No links, please
write a short paragraph explaining how the disease spreads among individuals. Include all of the following terms.
Susceptible host
Agent of disease
Reservoir
Portal of exit
Mode of transmission
Portal of entry

Answers

Answer:

Jfjfjdjdjxjdjfhfhfjfkfhfjdjfjfjdbjdjdjd

while looking at a graph, you notice a period of time where the line is perfectly horizontal what is most likely taking place during this time period

Answers

Answer:

The correct answer would be - the y-axis variable is constant.

Explanation:

In a normal line graph, there are generally two variables on two different axis one is at the x-axis and the other is on the y-axis that represents the correlation between these two variables. If there is a change in the variable present on the x-axis the change on the variable on the y-axis establishes the relationship among these variables.

If there is a period on the graph where the line is completely horizontal it means the variable present on Y-axis has no change during this period or is constant. Suppose a graph is plotted between speed or velocity on the Y-axis. A perfectly horizontal line on this graph means that speed is not changing over time and remain at te same speed.

Match the following

1. If not properly disposed of and treated, this can spread deadly bacteria.
soil
2. Drinking water that is contaminated by bacteria or chemicals can cause this.
100 gallons of water
3. Water pollution affects the earth's water supply as well as this.
water
4. The average person in America uses this each day.
water cycle
5. This occurs when livestock waste or crop chemicals enter the water supply.
sewage
6. This is the constant movement of water between the earth and the atmosphere.
agricultural pollution
7. Every living thing on earth needs this.
diarrhea

Answers

Answer:

1. Sewage

2. diarrhea

3. atmosphere

4. 100 gallons of water

5. agricultural pollution

6. water cycle

7. water

Explanation:

Answer:

1. Sewage, 2. Diarrhea, 3. Agricultural Pollution, 4. 100 Gallons of Water, 5. Soil, 6. Water Cycle, 7. Water. I took it on Edge so I know the answer. I hope you and everyone reading this have a good day!

Achieving sustainability of diverse ecosystems and resources in the ocean depends upon collective and individual actions. Describe what steps you would take to achieve this sustainability as a citizen.

Answers

Answer:

As a citizen, you would do things like help clean up the beaches, not use things that could pollute the ocean as much, or even spread awareness about the ocean’s pollution.

Explanation:

The role of the citizen is to help keep the ocean well in any way they can.

Biochemistry involves the production and degradation of biological molecules such as nucleic acids, proteins, lipids, carbohydrates, and vitamins. Changes in the production or degradation of these important molecules can cause disorders and diseases in humans. For each of the human disorders and diseases listed below, indicate whether it is caused by a change in: (A) nucleic acids (for example: DNA or RNA); (B) amino acids/proteins; (C) lipids (for example: cholesterol or triglycerides); (D) carbohydrates/sugars; or (E) vitamins. Provide only one (1) answer for each disease.

Answers

Answer:

Disorders are present below.

Explanation:

Degradation of nucleic acids causes Lesch-Nyhan syndrome and mitochondrial depletion syndromes in human body, degradation of proteins causes phenylketonuria and tyrosinemia diseases in humans, degradation of lipids causes Gaucher disease and Tay-Sachs disease. Due to these diseases of lipids, we have not enough enzymes to break down lipids, degradation of carbohydrates leads to diabetic ketoacidosis, hyperosmolar coma and hypoglycemia, and degradation of vitamins causes anemia and scurvy diseases in the body.

A. Somalia B. Kenya C. Spain D. Nigeria​

Answers

Answer:

Somalia

Explanation:

why would someone get tested for Osteogenesis Imperfecta

Answers

Osteogenesis imperfecta is tested for OI so if a family member has OI then a health care provider can test the DNA of the fetus for the presence of an OI mutation.

Answer:

Well I have osteogenesis imperfecta and I only got tested because I had broken 3 bones and I would just barely fall and it really didn’t make sense. So my mom demanded test

Explanation:

Match each step of the scientific method with its description.

Answers

Answer:

Sorry but

Explanation:

You didnt add an image its just the text, sorry

Joanne is going out to her car after taking a night class, and just as she is about to open the car door, a man appears who has his pants down to his knees and is masturbating. According to the text, Joanne's best immedaite response prior to notify campus security would be to:
A.scream loudly
B.stand still and watch for awhile
C.start laughing and tell him to put the silly thing away
D.calmly ignore the man

Answers

Answer:

D

because hes doing it for attention

D is the answer to your question!!

How can you tell from a food web which organisms will have the greatest biomass?

Answers

Answer:

You can tell by which animals are at the top of the food chain.

Explanation:

You can tell this because if you count all the biomass below the animals at the top of the food web (which eat all the biomass below it), there is much more biomass below these animals (the ones at the top of the food chain), than any other animal below it. For example, if the rat ate a grasshopper it will receive its biomass, but if the python then eats the rat, then the python will be getting the biomass of the rat and the grasshopper, compared to the rat just getting the biomass of the grasshopper. So since there is much more biomass below the animals at the top of the food web, this means that the animals at the top of the food web receive the most biomass (when they eat the animals below them) out of the whole food web individuals.

Bony Fish (bass etc.) and marine mammals (like dolphins) evolved some similar adaptations for survival in aquatic environments. However, they evolved separately and are not closely related evolutionarily. What type of evolution does this represent?

Answers

Answer:

Convergent evolution

Explanation:

Convergent evolution is a type of evolution of similar features and/or structures between organisms that are not phylogenetically related. This type of evolution is known to create analogous structures/organs that exhibit similar or the same functions but were not present in the last common ancestor of these taxa. An example of analogous structures (and therefore also of convergent evolution) are the wings of bats and of insects (e.g., butterflies). Conversely, divergent evolution is a type of evolution where species phylogenetically related, i.e., species that share a common ancestor, evolve and accumulate differences over time.

PLEASE HELP ASAP!! WILL MAKE YOU THE BRAINLIEST!!!
What is a trophic level?
a group of organisms that can convert one form of energy into another form
the total amount of energy that is present in an ecosystem
an organism's environment, as well as physical factors that make up that environment
a living organism's position in the flow of energy through an ecosystem

Answers

Answer:

A living organism's position in the flow of energy through an ecosystem.

After a forest fire, what would happen first?
HELP PLEASEw

Answers

i think the answer is B.

please solve my question

i will mark u as brilliant



Given below are a few activities carried out by agricultural farming.

1. Adding fertilizer or manure to the soil.

2. Removing the husk to feed cattle.

3. Removing weeds from the field

Which of the above helps to increase the amount of nutrients available to the crop plant?

A) Only 1 b) Only1 and 2

c) Only 1 and 3 d) All: 1, 2. And 3

Answers

Answer:

Adding fertilizer or manure to the soil.

Explanation:

This is because fertilizer or manure are formulated substance that contain different nutrients which when applied to the soil will add nutrients to the soil and make nutrients available for plants absorbing and these nutrients absorbed will help improve yield. They contain nutrients that help boost plants growth.

Mitosis is the process through which a cell replicates its DNA and produces two identical daughter cells. If a cell undergoing mitosis is currently in anaphase as shown in the diagram below,

Answers

B because I’m in 7th grade I learned abt this

The duplicated genetic information found in a parent cell's nucleus is divided into two identical daughter cells during the fourth stage of mitosis, referred to as anaphase. Thus, option B is correct.

What mitosis process through which cell replicates its DNA?

The sister chromatids are able to separate because the protein “glue” holding them together is degraded. Each chromosome now exists on its own. The opposing ends of the cell are drawn to the chromosomal pairs in each pair.

The replication fork is connected to a number of proteins that aid in the  The way DNA polymerase creates new strands is mostly by include nucleotides that complete each (template) strand. The S-stage of interphase is when DNA replication happens.

Therefore, Sister chromatids split from one another and are drawn to the opposite ends of the cell during anaphase.

Learn more about mitosis here:

https://brainly.com/question/11473163

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Which of the following is believed to be common among all mass extinction events?(1 point)

a significant change in the global climate

the type of species that went extinct

the type of event that causes the extinction

the number of species that went extinct

Answers

Answer:

D the type of event that causes the extinction

The correct answer is a significant change in the global climate, as due to this is believed to be common among all mass extinction events.

Climate extrude interacts with threats which include habitat loss and overharvesting to similarly exacerbate species declines. The decline of species and ecosystems can then boost up weather extrude, growing a remarks loop that similarly exacerbates the situation.

What is global mass extinction?

The mass extinction is an end result of human activity, pushed through populace boom and overconsumption of the earth's herbal resources. The 2019 international biodiversity evaluation through IPBES asserts that out of an expected eight million species, 1 million plant and animal species are presently threatened with extinction.

Therefore it is clear that a significant change in the global climate is believed to be common among all mass extinction events.

To learn more about mass extinction refer to the link ;

https://brainly.com/question/1048615

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A rental car company charges $53.75 per day to rent a car and $0.12 for every mile driven. Jaya wants to rent a car, knowing that: She plans to drive 125 miles. She has at most $230 to spend. Write and solve an inequality which can be used to determine xx, the number of days Jaya can afford to rent while staying within her budget. Solve the equation. Then check your solution. two-fifths + a = One-fifth a. Three-fifths c. StartFraction 3 Over 10 EndFraction b. Negative one-fifth d. Negative three-fifths Please select the best answer from the choices provided A B C D What happens when people are overly motivated by what other people think and say? Some ways aptitude can be learned includeborrowing itbuying itformal educationexperience What is the graph for y = -x + 10x - 23 What does the quotient rule mean? subtracting ,adding ,multiplying or dividing !!!!!MAJOR HELP PLS!!!!!!!!!!I will give 1 Brainly!!!!!What is the function equation for the input/output table?X|Y2|105|138|1611|19A.y=8xB.y=x+8C.y=5xD.y=x+3Image example is above Discuss five possible reason why some people in your community do not participate in New York campaign anybody randomly start listening to 2000's music boppin to beyonce rn. Find a polynomial f(x) of degree 5 that has the following zeros. 1, 8, -7, 5, -4. Leave your answer in factored form two similar triangles are shown. what is the value of x? pls help. What is the measure of BD? Need this done as soon as possible. Please DONT use g00gle translate as this is an exam grade and needs to be authentic. Thank you. Part CTaking into account the Aborigines' Dreamtime tradition, why did native Aborigines view the European colonization of Australia as a belligerentaction? Scientists use various methods to determine the age of matter. Which method below would a scientist most likely use to determine the absolute age of a rock layer? 1.Identify the oldest fossilized remains in the rock layer.2.Measure the magnetic strength of the material in a sample of the rock.3.Compare the amount of stable daughter elements present with the amount of radioactive parent elements.4.Compare the composition of the rock sample with other rock samples of known age. what makes festival and Phillipine theater important to the lives of the Filipinos? why? Fassst. A or b or c or d plzzz ........................................................ Calculate the amount of heat energy is needed to raise the temperature of 5.00 grams of lead from 25.0 to 35.0 degrees Celsius, if the specific heat capacity of lead is 0.129 J/g/C. Find the measure of ZC. Write your answer as a decimal.