In the alkaline phosphatase assay, you will you be monitoring the formation of product by measuring the absorbance of the solution using a spectrophotometer. Why are you monitoring the formation of product at a pH of 9 and a wavelength of 400nm.
A. At pH 9, the product, p-nitrophenol, will be ionized, the solution will appear yellow in color, and thus can be monitored at the wavelength of maximum absorption for the phenolate ion which is 400nm.
B. At pH 9, the product, p-nitrophenol, will be ionized and colorless
C. At pH 9, the product, p-nitrophenol, will be un-ionized and yellow in color

Answers

Answer 1

Answer:

a. At pH 9, the product, p-nitrophenol, will be ionized, the solution will appear yellow in color, and thus can be monitored at the wavelength of maximum absorption for the phenolate ion which is 400nm

Explanation:

In alkaline phosphatase assay, the hydrolysis of p nitrophenyl phosphate to p nitrophenol happens. When the ph is 9, the product which is p nitrophenol would undergo ionization. The solution is going to appear to be of yellow and it can be monitored at a wavelength for maximum absorption of phenolate ions at 400nm.

Option A is the answer to the question.


Related Questions

Four stereoisomers exist for 4-hexen-3-ol. Draw the structure of the isomer that has the Z configuration around the double bond and the R configuration at the chiral center.

Answers

Answer:

Following are the solution to the given question:

Explanation:

Please find the complete question and its graph file in the attachment file.

The isomer having Z specification from around dual connector as well as the chiral center S structure is:

[tex](3S,4Z)-4-Hexen-3-ol[/tex]

An 'H' atom has been at the level of 3rd carbon, and we need to change its configuration, through 'R' to 'S' by CIP notation (under contrast to -OH group).

Answer:

The double bond can have either the E or the Z configuration.

Each of these stereoisomers can exist in either the R or the S configuration at the chiral center for a total of four possible stereoisomers.

name this structure ​

Answers

Answer:

The fourth one- bromo trifluoro methane

Explanation:

Answer:

D

Explanation:

I believe the answer is the 4th option, bromo-trifluoro-methane

What is the first step in this
experiment?

Answers

Cutting the cardboard would be the first step

Answer: Cut the card borad

Explanation:

gA scientist is trying to discover information about an unknown metal in a compound. The formula for the compound is believed to be XBr3XBr3 where XX is the unknown metal. The scientist determined that a 4.700 g4.700 g sample of this compound contains 4.834×10−2 mol Br4.834×10−2 mol Br . Calculate the atomic mass of the unknown metal, XX .

Answers

Solution :

It is given that :

The formula of the compound = [tex]$XBr_3$[/tex]

And that 4.70 g of the sample contains [tex]$4.834 \times 10^{-2}$[/tex] mol of Br.

It means that :

1 mol of [tex]$XBr_3$[/tex] contains = 3 mol of Br

∴ 3 mol of [tex]$Br^-$[/tex] contain in 1 mol of [tex]$XBr_3$[/tex]

[tex]$4.834 \times 10^{-2}$[/tex] mol of [tex]$Br^-$[/tex]  contains [tex]$1.576 \times 10^{-2}$[/tex] mol of [tex]$XBr_3$[/tex]

Thus the mol of [tex]$XBr_3$[/tex] = [tex]$1.576 \times 10^{-2}$[/tex] mol

The given mass is = 4.700 g

Therefore, the [tex]$\text{molar mass}$[/tex] of [tex]$XBr_3$[/tex]  [tex]$=\frac{\text{mass}}{\text{mol}}$[/tex]

                                                           [tex]$=\frac{4.700 \ g}{1.576 \times 10^{-2} \ mol}$[/tex]

                                                          = 298.4 g/mol

So [tex]$\text{molar mass}$[/tex] of [tex]$XBr_3$[/tex] = [tex]$\text{molar mass}$[/tex] of X + 3 x [tex]$\text{molar mass}$[/tex] of Br

      298.4 g/mol = [tex]$\text{molar mass}$[/tex] of X + 3 x 79.90 g/mol

      298.4 g/mol = [tex]$\text{molar mass}$[/tex] of X + 239.7 g/mol

       [tex]$\text{molar mass}$[/tex] of X = 58.71 g/mol    (since 1 amu = 1 g/mol)      

Therefore the atomic mass of the unknown metal = 58.71 g/mol

So the unknown meta is Nickel.

what is the definition of drugs​

Answers

Answer:

Drugs is a type of medication

Another meaning for it is something that makes you feel drunk such as wine, alcohol and as such that causes addiction.

Please help me thanks:)

Answers

Answer:

where it goes

Explanation:

Answer:

I didn't do the observation that the question said so I can't help sorry

How does the reactivity of nonmetals change as you move from left to right across the periodic table?

Answers

Answer:

Metallic character refers to the level of reactivity of a metal. Non-metallic character relates to the tendency to accept electrons during chemical reactions. Metallic tendency increases going down a group. Non-metallic tendency increases going from left to right across the periodic table.

Explanation:

I hope this helps :)

Answer:

Explanation:

Non metals increases in reactivity!

Which describes the proper way to use an eye wash station?
Keep your contact lenses in place.
Press on your eyelids firmly.
Rinse your eyes for fifteen minutes.
Call the paramedics for instructions.

Answers

Answer:

C

Explanation:

Rinsing your eyes thoroughly is the proper way to was your eyes out.

1. Take your contacts out as they can get stuck in your eye.

2. Don’t press on your eyes as you will just rub in the chemical substance more.

3. Can’t call the paramedics as you should rinse your eyes immediately.

Rinse your eyes for 15 min

When a solid turns into a liquid what is happening to the relationship between the forces?

Answers

The particles will move faster and further apart. There will be more energy in the particles due to the heat.

The process of determining the composition of a sample by conducting chemical tests is called qualitative analysis.

a. True
b. False

Answers

I think it’s actually called an ElementaI analysis, so this would be false, hope this helps.

g You are given an aqueous solution of a sodium halide salt (NaCl, NaBr, or NaI). You have a solution of silver nitrate and a solution of ammonia available. Describe an experimental procedure to determine which of the three salts is present in the solution.b. Chlorine water (made by bubbling Cl2 (g) through water) is added to a solution of a sodium halide salt. Hexane is then added, and the mixture is gently agitated. The hexane layer turns purple. What is the identity of the salt-- NaCl, NaBr, or NaI

Answers

Answer:

See explanation

Explanation:

If i have a sodium halide salt which can be any of NaCl, NaBr, or NaI, addition of  AgNO3 and ammonia leads to the formation of various colour of precipitate which helps to identify the particular halogen atom that is combined with sodium.

If the sodium halide is NaCl, a white precipitate is formed when AgNO3 is added. The precipitate is soluble in ammonia solution.

If the sodium halide is NaBr, a cream precipitate is formed when AgNO3 is added. This precipitate is sparingly soluble in excess ammonia solution.

If the sodium halide is NaI, a yellow precipitate is formed when AgNO3 is added. This precipitate is insoluble in excess ammonia solution

b) The true color of a halogen is only observed when it is viewed in hexane. iodine in hexane has a purple colour. Hence, the reaction that occured is;

NaI(aq) + Cl2 →  NaCl(aq) + I2

Why are science safety rules important? PLZ DO NOT COPY AND PASTE

Answers

It is important to follow them because they keep us safe when we’re doing experience we have to follow specific rules because these rules keep us safe from doing some thing that could possibly go wrong hope that helps

A__ is two or more substances that are together in the same place but are not chemically ( combined.​

Answers

Answer:

yeah

Explanation:

Image result for The highest level of organization for living things is the biosphere; it encompasses all other levels. The biological levels of organization of living things arranged from the simplest to most complex are: organelle, cells, tissues, organs, organ systems, organisms, populations, communities, ecosystem, and biosphere.

The highest level of organization for living things is the biosphere; it encompasses all other levels. The biological levels of organization of living things arranged from the simplest to most complex are: organelle, cells, tissues, organs, organ systems, organisms, populations, communities, ecosystem, and biosphere.

A cell is placed in a salt solution that has the same concentration as the inside of the cell. What will happen to the cell

A. The cell will contract
B. The cell will expand slightly
C. The cell will burst
D. The cell will remain the same size

By the way I had to ask again because I f forgot to put the answer choices​

Answers

Answer:

B

Explanation:

If not B then its d

Answer:

D

Explanation:

This would be an isotonic solution. If a cell is placed in a solution of salt with the same concentration of salt in the cell, nothing will happen.

A chemical reaction takes place inside a flask submerged in a water bath. The water bath contains 3.30kg of water at 23.8°C. During the reaction 112.kJ of heat flows out of the bath and into the flask. Calculate the new temperature of the water bath. You can assume the specific heat capacity of water under these conditions is 4.18·J·g−1K−1. Round your answer to 3 significant digits. °C

Answers

Answer:

[tex]T_2= 31.9\°C[/tex]

Explanation:

Hello there!

In this case, it is possible to propose an energy balance in order to illustrate how the heat released by the reaction is absorbed by the water:

[tex]-Q_{rxn}=Q_{water}[/tex]

Thus, since the heat released by the reaction is -112 kJ (-112000 J), it is possible to define the hear absorbed by the water in terms of mass, specific heat and temperature change:

[tex]-(-112000J)=m_{water}C_{water}(T_2-T_1)[/tex]

In such a way, it is possible to define the final temperature as shown below:

[tex]T_2=23.8\°C+\frac{112000J}{3300g*4.18\frac{J}{g\°C} }\\\\T_2= 31.9\°C[/tex]

Best regards!

What is the boiling point in °C of a 3.6 molal solution of calcium chloride in water?

Answers

Answer:

CaCl2--->Ca2+ + 2Cl_ so i=3

3. Methyl acetate is hydrolyzed at 25 oC in acidic environment. Aliquots of equal volume are removed and titrated with NaOH solution. The hydrolysis reaction is irreversible. The results are Time/s 339 1242 2745 4546 infinite Volume of base/ mL 26.34 27.80 29.70 31.81 39.81 Write the reaction of hydrolysis of methyl acetate. (3 pts.) Neglect the reverse reaction. Find the order of the hydrolysis reaction and the value of the rate constant at this temperature. (12 pts.) Total 15 pts.

Answers

Solution :

Time (sec)       Volume of NaOH (mL)

339                           26.23

1242                         27.80

2745                        29.70

4546                         3.81

[tex]$\infty$[/tex]                               39.81

Now the example of the first order kinetics w.r.t volumetric analysis is :

[tex]$k=\frac{2.303}{t} \log \left(\frac{v_{\infty}-v_0}{v_{\infty}- v_t}\right)$[/tex]

Here, [tex]$v_{\infty}= \text{ volume at }\infty = 39.81$[/tex]

[tex]$v_{t}= \text{ volume at time 't' } = 27.80$[/tex]

[tex]$v_0$[/tex] = volume at time 0 = 0

Since the interval is not constant, we take the time interval as

[tex]$=\frac{903+1503+1801}{3}$[/tex]

[tex]$=\frac{4207}{3}$[/tex]

= 1402.3333

≈  1402 seconds

[tex]$k=\frac{2.303}{1402} \log \left(\frac{39.81-0}{39.81-27.80}\right)$[/tex]

  [tex]$=(0.001643) \log \left(\frac{39.81}{10.01}\right)$[/tex]

  = 0.001643 x 0.52045

  = 0.00082

  [tex]$= 8.55 \times 10^{-4} \ sec^{-1}$[/tex]

Therefore, the first order rate constant is k [tex]$= 8.55 \times 10^{-4} \ sec^{-1}$[/tex].

A 0.00215-g sample of polystyrene, a polymer composed of carbon and hydrogen, produced 0.00726 g of CO2 and 0.00148 g of H2O in a combustion analysis. What is the empirical formula for polystyrene?

Answers

Answer:

CH

Explanation:

Polystyrene is composed only by C and H.

We can calculate the number of C moles contained in the original sample mass (0.00215 g), using the produced mass of CO₂:

0.00726 g CO₂ ÷ 44 g/mol = 1.65x10⁻⁴ mol CO₂

As 1.65x10⁻⁴ moles of CO₂ were produced, in the polystyrene sample there were 1.65x10⁻⁴ moles of C.

Now we calculate how many moles of H₂O were produced:

0.00148 g H₂O ÷ 18 g/mol = 8.22x10⁻⁵ mol H₂O

The number of H moles produced is double the amount of H₂O moles, so in the polystyrene sample there were 1.64x10⁻⁴ moles of H.

The number of C and H moles are the same, this means the empirical formula is CH.

In the combustion of gasoline, shown below, if 15.0 g of CO2 is produced, what volume of oxygen gas would be consumed? Assume STP. You must show your work.

Answers

Answer:

Depends on what you are burning. If it is carbon (ie coal), the answer is 15 (one liter CO2 for each liter of oxygen. Simply add a C onto an O2.) If you are burning octane, (C8H18), for each carbon you would you would have 1 1/8 H2Os and one CO2. To round off slightly (as I do not feel like getting out my calculator) we would have about twice as much oxygen tied up in CO2 as H20 (as there is only one O in H2O vs 2 in CO2). Therefore, in this case, it is ~10 l of CO2 for 15 l of oxygen. Propane will be different again, as will natural gas, etc.

Explanation:

You have a container which has the capacity to hold 3 L water. If it already has 2.25 L water in it, how much more water in mL can you still add to fill it completely?

Answers

3-2.25 = 0.75 L
0.75 L = 750 mL

According to the following reaction, how many grams of iron(II) chloride will be formed upon the complete reaction of 21.6 grams of hydrochloric acid with excess iron?
iron (s) + oxygen (g) Arrow.gifiron(II) oxide (s)

Answers

Answer:

37.58 g of FeCl₂.

Explanation:

We'll begin by writing the balanced equation for the reaction. This is illustrated below:

Fe + 2HCl —> FeCl₂ + H₂

Next, we shall determine the mass of HCl that reacted and the mass of FeCl₂ produced from the balanced equation. This is can be obtained as follow:

Molar mass of HCl = 1 + 35.5 = 36.5 g/mol

Mass of HCl from the balanced equation = 2 × 36.5 = 73 g

Molar mass of FeCl₂ = 56 + (2×35.5)

= 56 + 71

= 127 g/mol

Mass of FeCl₂ from the balanced equation = 1 × 127 = 127 g

SUMMARY:

From the balanced equation above,

73 g of HCl reacted to produced 127 g of FeCl₂.

Finally, we shall determine the mass of FeCl₂ produced by the reaction of 21.6 g of HCl. This can be obtained as follow:

From the balanced equation above,

73 g of HCl reacted to produced 127 g of FeCl₂.

Therefore, 21.6 g of HCl will react to produce = (21.6 × 127)/73 = 37.58 g of FeCl₂.

Thus, 37.58 g of FeCl₂ were obtained from the reaction.

Diffusion is the movement of water between the cell membrane.
O True
O False​

Answers

it is true good friend chip chip cherrio

True good luck hope it helps

How is science based on observation used as evidence? How can this evidence be used?

15 POINTS AND BRAINLIEST

Answers

Answer:

w

Explanation:

Well in science and observing you are studying and looking more in depth to something. When observing what is going on you are experiencing it with your own eyes so one may record and/or write down what is happening.

Bobota
Level 1
Q2
What is the ability of a substance to
burn or ignite, causing fire or combustion?
DANGER
A. Chemical stability
B Reactivity
C Flammability
D. Conductivity

Answers

Answer:

chemical stability

Explanation:

g goodnight

why is weather forecasting important to the people​

Answers

Answer:

Weather forecasting is important to the people since it helps determine future climate expectations .

Chose the element pairings that are likely to react with each other
Na and K or Na and Br

Answers

Answer:

Na k

Explanation:

because na is a metal and potassium is also a metal and both are active metal so is less likely to react as no bond is formed between them

Where do most of the visible electromagnetic waves on Earth come from?

Question 3 options:

x-rays


the sun


radio waves


florescent lights

Answers

Answer:

the sun

Explanation:

hope this helps. :) brainlyest would be nice as well but it's ok if not, thank you!

Answer: the sun

Explanation:

3x + 4y - 3z) - (2x - 6y + 7z)

x + 10y - 10z
x + 10y - 10z

x -10y - 10z

Answers

Answer: x + 10y - 10z is the answer

Explanation:

Question 5

1 pts

What is the correct interpretation of an 85% yield for a given reaction?

85% of the anticipated amount of products were produced (relative to the theoretical yield)

O 15% of the reactants were used up

O 85% of the reactants were used up

15% of the anticipated amount of products were produced (relative to the theoretical yield)

Question 6

1 pts

True or false: The limiting reactant is the reactant that is used up after the reaction is complete.

O True

False

Answers

Answer: a) 85% of the reactants were used up

b) True

Explanation:

Percentage yield is defined as the ratio of actual yield to theoretical yield in terms of percentage. The actual yield is the amount of product obtained after the experiment and the theoretical yield is the amount of ideal product that should have been obtained.  

Formula :  

[tex]\text {percentage Yield}=\frac{\text {Actual Yield}}{\text {Theoretical Yield}}\times 100[/tex]

Thus 85 % yield means 85 % of the reactants were used up to give only 85% of products.

The limiting reactant is the reactant that limits the amount of product formed and hence is completely used up after the reaction is complete. Thus the given statement is True.

The equilibrium constant, Kc, is calculated using molar concentrations. For gaseous reactions another form of the equilibrium constant, Kp, is calculated from partial pressures instead of concentrations. These two equilibrium constants are related by the equation Kp=Kc(RT)Δn where R=0.08206 L⋅atm/(K⋅mol), T is the absolute temperature, and Δn is the change in the number of moles of gas (sum moles products - sum moles reactants). For example, consider the reaction N2(g)+3H2(g)⇌2NH3(g) for which Δn=2−(1+3)=−2. Part A For the reaction 2A(g)+3B(g)⇌C(g) Kc = 63.2 at a temperature of 81 ∘C . Calculate the value of Kp. Express your answer numerically.

Answers

Answer:

Kp = 1.41 x 10⁻⁶

Explanation:

We have the chemical equation:

2 A(g) + 3 B(g)⇌ C(g)

In which A and B are the reactants and C is the product. We calculate first the change in the number of moles of gas (Δn or dn):

dn= (sum moles products - sum moles reactants)

   = (moles C - (moles A + moles B))

   = (1 - (2+3))

   = 1 - 5

   = -4

We have also the following data:

Kc = 63.2

T= 81∘C + 273 = 354 K

R = 0.082 L.atm/K.mol (it is a constant)

Thus, we introduce the data in the mathematical expression for the relation between Kp and Kc:

[tex]Kc = (RT)^{dn}[/tex]= (0.082 L.atm/K.mol x 354 K)⁻⁴ = 1.41 x 10⁻⁶

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