Determine the correct sequence of events during the creation of recombinant DNA.
I. Plasmid is cut open with enzymes
II. Target gene is cut out with enzymes
III. Plasmid is inserted into the bacterium
IV. Target gene is bonded to the plasmid with Ligase
A. IV, I, II, III
B. II, I, IV, III
C. I, II, III, IV
D. IV, III, I, II

Answers

Answer 1

Answer:

I believe the correct answer to your question is A

IV, I, II, III


Related Questions

Areas on the moon called maria Select one: a. are seas of water. b. consist of basaltic lava. c. formed a million years ago. Clear my choice

Answers

Answer:- B
Explanation:- Samples of lunar rock and soil brought back by Apollo astronauts proved that the maria are composed of basalt formed from surface lava flows that later congealed. ... The maria basins were formed beginning about 3.9 billion years ago during a period of intense bombardment by asteroid-sized bodies.

Areas on the moon called maria consists of basaltic lava. Option B is correct.


The areas on the moon called maria are not actually seas of water, as option a suggests. Instead, they are large, dark, flat areas on the moon's surface. These areas were formed by ancient volcanic activity.

Maria are made up of basaltic lava, which is a type of lava that is low in viscosity and can flow easily. When volcanic eruptions occurred on the moon in the past, this lava flowed out onto the surface and covered large areas, creating the maria.

These volcanic eruptions and the formation of the maria took place billions of years ago, not just a million years ago as option c suggests. They are remnants of the moon's early history when volcanic activity was more prevalent.

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Geologists might study everything except for a. the moon. b. fossils. c. minerals. d. hurricanes.

Answers

Geologists are trained professionals that might study everything except for the moon (Option a).

Who are Geologists?

Geologists are professionals who study the features of rocks and fossils that formed during natural processes.

Geologists also study the periods of time in which these fossils can have appeared on the Earth.

In conclusion, geologists are trained professionals that might study everything except for the moon (Option a).

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From watching his wife struggle to keep track of the family finances, Intuit founder Scott Cook identified the need for easy-to-use financial software that provided a single place for managing bills, bank accounts, and investments. This is an example of which innovator DNA trait

Answers

Answer:

It is observing

Explanation:

In observing innovator DNA trait, innovators or observers carefully and consistently search out for any little behavioral details in the activities of customers, suppliers, and other companies so that they will gain insights and knowledge about new ways of doing things.

From watching his wife struggle to keep track of the family finances, Intuit founder Scott Cook identified the need for easy-to-use financial software that provided a single place for managing bills, bank accounts, and investments is observing because he look out carefully for any little behavioral details in on how his wife handle business related financial activities in the home so that he will gain new insights on how to do things in a new way.

Gastrin, histamine, endorphins, serotonin, cholecystokinin, and somatostatin are hormones or paracrines that are released directly into the lamina propria. Which of the following cell types synthesize and secrete these products?
a) parietal cells
b) enteroendocrine cells
c) mucous neck cells
d) zymogenic cells

Answers

Answer:

The correct answer is option b, that is, enteroendocrine cells.

Explanation:

The specialized cells of the pancreas and the gastrointestinal tract exhibiting endocrine function are termed as enteroendocrine cells. These cells generate gastrointestinal peptides or hormones in response to a distinct kind of stimuli and discharge them into the bloodstream for the systemic effect. These are the most varied endocrine cells found within the body.  

The intestinal enteroendocrine cells produce somatostatin, cholecystokinin, motilin, enteroglucagon, and neurotensin hormones. The G cells present within the intestine produce gastrin. In the gastric glands, the gastric enteroendocrine cells are present, which produce histamine, and others like cholecystokinin, alpha and gamma-endorphin, somatostatin, and others.  

In the islets of Langerhans, the pancreatic enteroendocrine cells are present, which produce hormones like ghrelin, amylin, somatostatin, and pancreatic polypeptide.  

During an intramuscular injection procedure, the medication label should be checked:

Answers

Answer:

The medication label should be checked by comparing it with the  MAR (Medication Administration Records or therapeutic documentation care plan TDCP).That is the label  printed on the medication should be compare with the Medication  Administrations records  

Explanation:

Ideally this should be done three times, when it is removed from the medication cart,and before and after  it was removed form the  medication  cart.

Besides,the date of expiration should be checked,and the regulations concerning administrations of multi-does vial should be  taken into considerations.

The medications mode of storage should be taken into confederation,proper storage must be ensured.

Generally the application of medication through injection into the muscles for faster absorption  into the blood streams for fast distribution is called IM. This is aided by the rich blood supply to the muscles.Therefore it is a faster mode of blood application than the subcutaneous routes.

In your lab you are studying the genome of venomous rattlesnakes to find the gene which codes for their venom glands. You have two
rattlesnakes, one rattlesnake has a mutation but can still produce venom. You compare his DNA to a normal rattlesnake. What type of
mutation has occurred?
Normal: AATCGCTACGCACGTCAG
Mutated: TATCGCTACGCACGTCAG
A. Deletion Mutation
B. Nonsense mutation
C. Silent Mutation
D. Missense mutation

Answers

It is actually a mis-sense mutation.
ATA on DNA is UAU on mRNA. This codon has the amino acid ‘Leu’
TAT on DNA is AUA on mRNA. This codon has the amino acid ‘Ile’
A silent mutation means that the amino acid is not changed. This is a missense mutation because the amino acid is changed but the snake is still able to produce venom.

Perhaps you and/or your significant other is a carrier of a harmful recessive trait, such as cystic fibrosis. What would you do when considering having children? Would you hope for the best and ignore the situation, or would you see a genetic counselor or what?

Answers

Answer:

At birth the baby would indicate the FEIM laboratory examination or genetic analysis prior to delivery.

Explanation:

The FEIM laboratory examination is carried out by extracting a portion of blood from the newborn child, and is necessary for the accurate diagnosis of diseases such as cystic fibrosis and others such as HIV, etc.

Which of the following is a primary function of carbohydrates?

Choose ONE answer:

A. Acting as a long-term energy source.

B. Speeding up chemical reactions.

C. Storing genetic information.

D. Providing short-term energy storage.​

Answers

Answer: For me providing short - term energy storage is C so whatever providing short - term energy storage is on yours that's the correct answer. :D have a nice day.

Explanation: Carbohydrates are the main energy source of cells.

They provide a quick energy supply for all types of cellular activities.

The correct answer is

Providing short-term energy storage

The correct answer is option D. Providing short-term energy storage.​

What Do Carbohydrates Do to Your Body?

Carbohydrates are the main source of energy for your body: they help fuel your brain, kidneys, myocardium, and central nervous system. For example, fiber is a carbohydrate that aids digestion gives you a feeling of fullness, and lowers blood cholesterol levels.

Carbohydrate Function

Energy supply and blood sugar regulation.Gentle use of protein to generate energy.Degradation of fatty acids and prevention of ketosis.Biological recognition process.flavors and sweeteners.fiber.

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In order for translation to occur: Please choose the correct answer from the following choices, and then select the submit answer button. transcription must be occurring simultaneously. there must be large numbers of free amino acids present in the cytoplasm. the DNA must produce large numbers of free amino acids. replication must be occurring simultaneously. the nuclear membrane must first dissolve

Answers

Answer:

there must be large numbers of free amino acids present in the cytoplasm

Explanation:

In order for translation to occur, there must be a large number of free amino acids in the cytoplasm.

These free amino acids are usually carried by the transfer RNA which also carries the corresponding mRNA codon.

During translation, as the codons in the mRNA match their complements in the tRNA, the amino acid being carried by the tRNA is released and a peptide bond is formed between subsequent amino acids until the required polypeptide bond is formed.

Translation and transcription only occur simultaneously in prokaryotic cells where there is no barrier between the cell's genetic materials and the cytoplasm, unlike in eukaryotic cells where the genetic materials are housed in the membrane-bound nucleus.

Which flowchart correctly lists the structures in order from smallest to largest?
gene Right-arrow DNA Right-arrow chromosome Right-arrow nucleus
gene Right-arrow chromosome Right-arrow DNA Right-arrow nucleus
DNA Right-arrow gene Right-arrow chromosome Right-arrow nucleus
DNA Right-arrow chromosome Right-arrow gene Right-arrow nucleus

Answers

Answer:

gene Right-arrow DNA Right-arrow chromosome Right-arrow nucleus

Explanation:

trust me I'm smart

Listing the structures in order from smallest to largest follows this sequence ( A ) ; Gene right arrow DNA right arrow Chromosome right arrow Nucleus

Gene is the basic unit of heredity in animals, they are made up of DNA strands, while chromosomes ( thread like structures ) are positioned inside the Nucleus of ( plant and animal cells ).

Chromosomes are comprised of proteins and single molecule DNA that are passed from parents to its offspring.

Hence we can conclude that the flowchart that lists the structures in order from smallest to largest follows this sequence ( A ) ;  Gene right arrow DNA right arrow Chromosome right arrow Nucleus.

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What two characteristics of life are evident in viruses?



Please help me with this question

Answers

Answer:

Viruses do, however, show some characteristics of living things. They are made of proteins and glycoproteins like cells are. They contain genetic information needed to produce more viruses in the form of DNA or RNA. They evolve to adapt to their hosts.Living characteristics of viruses include the ability to reproduce – but only in living host cells – and the ability to mutate.

Which of the following is not a method of cell signaling?
A. Short range message molecules
B. Direct contact
C. Hormones
D. Enzymes

Answers

Answer:

C: Hormones

Explanation:

hormones do not "signal" other cells. I hope this helps :)

The correct answer is option C. Hormones.

What is the simple definition of hormones?

Hormones are chemicals that act like messenger substances in the body. After being made in one part of the body, they move to other parts of the body, where they help control how cells and organs work. For example, insulin is a hormone made by the beta cells of the pancreas.

In endocrine signaling, signaling molecules (hormones) are secreted by specialized endocrine cells and transported through the circulatory system to affect target cells in remote parts of the body.

Hormones are so many essential parts of your body's system that they regulate much of what your body does. Hormones regulate growth, libido, sexual development, reproduction, sexual function, metabolism, and thirst, among other things. They simply tell the body what to do to keep it running smoothly.

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What impact does altitude have on pressure?

Answers

Answer:

As altitude increases, the amount of gas molecules in the air decreases—the air becomes less dense than air nearer to sea level.

Explanation:

Thin air exerts less pressure than air at a lower altitude.

Some individuals can be heterozygous for a particular mutation.This means that one of their chromosomes is normal and the other is mutated in some way. Approximately ___ of the gametes produced by an individual heterozygous for a deletion will be non viable.

Answers

Answer:

1/2 (50%)

Explanation:

During meiosis, homologous pairs of chromosomes are separated in order to form haploid cells, and this separation or assortment is known to be a random process. In consequence, the chromosome carrying the mutation will have a 1/2 (50%) chance of being inherited in the gamete that, after fertilization, produces a non-viable organism. In consequence, the non-mutated chromosome also will have a 1/2 chance of occurring in the offspring

Nancy Scheper-Hughes and Margaret Lock observed students at a teaching hospital trying to diagnose a woman's debilitating headaches. Students were impatient with the woman's description of her life circumstances, including an abusive husband and an ailing mother-in-law, but her description might BEST be termed as which of the following?
a. an illness narrative
b. a biomedical diagnosis
c. the human microbiome
d. alternative medicine

Answers

Answer:

The answer is option A.

Explanation:

An illness narrative is defined as "patients’ stories about their experiences of illness" on Oxford Medicine. According to the information given in the question, she is telling her experience in an autobiographical fashion with the environmental details, description and other things that may affect her situation, so her description can be best categorized as an illness narrative. The answer is option A.

I hope this answer helps.

The wells that contain the DNA samples are placed next to the negatively charged electrode in the electrophoresis box (chamber) because _______. Group of answer choices DNA strands have both a positive and a negative charge DNA is negatively charged DNA is positively charged DNA has no electrical charge

Answers

Answer:

DNA is negatively charged

Explanation:

Electrophoresis: In biology, the term "electrophoresis" is described as one of the different laboratory technique that is being utilized to separate protein molecules, DNA, and RNA one the basis of their electrical charge and size. Therefore, a specific amount of "electric current" is being used to move different molecules that are required to be separated through a "gel". The given gel contains pores that generally works like a "sieve" and thus allows every small molecule to proceed faster as compared to the larger molecules.

DNA stands for "deoxyribonucleic acid" is determined as a long molecule that ought to contain an individual's unique "genetic code" and generally carried information associated for making proteins in an individual's body.

In the question above, the correct answer is "DNA is negatively charged".

The samples of DNA are placed next to the negatively charged electrode in the electrophoresis as DNA is negatively charged.

What is electrophoresis?

It is a laboratory technique, which is used to distinguish RNA, DNA or molecules of protein on the basis of their electrical charge and size. In the process, an electric current is utilized to move molecules to be separated via  a gel. The gel comprises pores that functions as a sieve, which permits smaller molecules to move faster in comparison to larger molecules.

DNA is a negatively charged molecule, thus, when it is used in the process of electrophoresis, the migration of DNA will take place towards the positively charged electrode. Through the gel, the DNA with shorter strands will move more briskly in comparison to the longer strands leading to the formation of fragments being aligned in order of size.

Thus, the samples of DNA are placed next to the negatively charged electrode as they are negatively charged.

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what do scientist need to look at before devleping an argument

Answers

Answer:

Because they are genuis

Explanation:

8. What type of chemical bond forms between positive and negative ions?
metallic
jonic
covalent

Answers

Answer:

and: option B) Ionic bond

URGENTT!
does a fire take food for energy?
does a fire reproduce?
does a fire produce waste?

Answers

Answer:

Yes, a fire takes food for energy.

No, a fire doesn't reproduce.

Yes, a fire produces waste.

Explanation:

For fire, the food that it requires is the objects that help in the process of burning. The objects can be wood, paper, coal, fuel, etc. Oxygen is required for the process of burning to take place.

The process of reproduction undergoes in living beings. Fire produces heat and light but this cannot be termed to be a process of reproduction.

Burning produces wastes in the form of ash and burned products. Therefore, it can be said that fire produces wastes.

Which of the following membrane-enclosed organelles contains several oxidases that are involved in oxidation of fatty acids and amino acids during normal metabolism and in detoxification of chemicals like alcohol in the liver?
a) peroxisomes
b) mitochondria
c) proteasome
d) ribosomes
e) lysosomes

Answers

Answer: The correct answer is a) Peroxisomes.

Explanation: This kind of membrane enclosed organelles has a mainly function that is the oxidation, this source manages to break fatty acids and during normal metabolism, they also has many enzimes like d-aminoacid oxidases, is another of their functions. Further, the catalase enzime (another enzime which is inside peroxisomes) manages the detoxification of chemicals like alcohol from alcoholic drinks (catalases transform the H2O2, which is toxic, into H2O to detoxificate the liver).

Which statement is correct? Carbon dioxide diffuses from the alveoli into surrounding capillaries. In the blood, oxygen is bound to hemoglobin, a protein found in red blood cells. Oxygen is released from the mitochondria as a product of cellular respiration. As oxygen diffuses from the lungs into capillaries, blood becomes deoxygenated. Oxygen diffuses from large blood vessels into the body's cells.

Answers

Answer:

In the blood, oxygen is bound to hemoglobin, a protein found in red blood cells.

Explanation:

The blood contains a protein called hemoglobin. This protein has the important use of ensuring oxygen is bound to the blood for distribution to other parts of the body. The oxygen helps in the vital functioning and metabolism of the various body parts.

This explains why In the blood, oxygen is bound to hemoglobin, a protein found in red blood cells Is the right choice.

As células são os componentes fundamentais de todos os seres vivos, não sendo encontradas apenas nos vírus. Algumas pessoas costumam dizer que as células são formadas por membrana plasmática, citoplasma e núcleo. Entretanto, nem todas as células possuem núcleo organizado, recebendo o nome de:

Answers

Answer:

Entretanto, nem todas as células possuem núcleo organizado, recebendo o nome de: células eucarióticas

Explanation:

As células eucarióticas são caracterizadas por possuir um núcleo definido, localizado no citoplasma. A diferença que tem com as células procarióticas é que o núcleo nelas está em uma região do citoplasma chamada nucleoide.

Além disso, as células eucarióticas têm um nível mais alto de complexidade em relação à sua informação genética, podendo realizar dois processos para replicar seu DNA: mitose e meiose

Why is the frequency of natural disasters increasing? Select one: a. There are less natural hazards occurring. b. Human population in areas prone to natural hazards has increased. c. Deaths from natural disasters has decreased in developed countries and increased in developing countries.

Answers

Answer:

I hope option c is the correct answer.

Explanation:

As for option 1 its totally in correct.

for option 2 human population is not always greater in the disaster places.

for option three developed countries are developed with all the determined factors but developing countries dont see all the factors as they destroy nature while making road which causes landslide, which causes death.

Hope it helps...

Answer:

Option C

Explanation:

I believe Option C is the correct answer

What could happen if the cell cycle for the cells in your pinky finger stopped functioning properly? (Check all that apply.)

Answers

Answer:

Hello, you did not enter the answer options, but I can help you by explaining what would happen in this case.

if the cell cycle of the cells in your little finger stops working properly, two things could happen. First, if these cells were unable to start and complete the cell cycle, your finger would be unable to heal cuts and bruises that could happen to it, on the other hand, if these cells were unable to end the cell cycle, your finger would develop cancer .

Explanation:

The cell cycle allows the cells of your circus, in this case, of your little finger, to multiply and create exactly the same cells. This process is very important to not only make your finger grow, but also to be able to heal cuts and bruises, regenerating the tissues and skin of your finger.

Unfortunately, over time, or as a result of some factors, cells may have defects in the cell cycle. In short, if the cells are unable to start and complete the cell cycle, your finger will be unable to create new cells and regenerate tissues and skins if your finger is cut or injured. In addition, the cells may not be able to finish the cell cycle and multiply in an exaggerated and unregulated way, generating a cancer in the finger.

A typical eukaryotic nucleus has to exchange a great deal of protein and nucleic acid material with the surrounding cytosol of the cell. Which choice correctly describes a feature of most nuclei that helps promote this exchange

Answers

Answer: A typical Eukaryote has an organized nucleus as well as an organized cell organelles.

The nucleus is enclosed in a nuclear envelope and the whole cell organelles are further enclosed in a plasma membrane (cell membrane).

From the question,the choice that correctly describe the feature of most nuclei that helps to promote the exchange of protein and other nucleic acids between the nucleus and the surrounding environment is the presence of nuclear pores which allows for selective movement of materials/items in and out of the nuclear space in a regulated manner.

What did Chargaff discover while studying bases in the DNA of organisms?

Answers

Answer: The ratios of thymine and adenine were similar, as were the ratios of guanine and cytosine.

Explanation:

Proteins play key roles in olfaction. One kind of protein, called ___________, attaches itself to hydrophobic odorant molecules so that they may dissolve in watery mucous. A second class of proteins, to which odorant molecules become bound and initiate transduction, are coded by the largest family of genes in mammals and are called ___________ . Group of answer choices

Answers

Answer:

The correct answer is "Odorant-binding proteins (OBPs); Olfactory receptors (ORs)".

Explanation:

Odorant-binding proteins (OBPs) are low-molecular soluble molecules that are secreted in high amounts in the nasal mucus layer. The precise biological activity of OBPs remains unclear, however, its solubility and ability to attach to hydrophobic odorant molecules suggest that they acts as carriers. A second class of proteins called olfactory receptors (ORs), act at the genetic level by initiating transduction once odorant molecules attach to them. ORs are coded by the largest family of genes, which include more than 100 genes and pseudogenes that represent around 3% of the genome.

Feedback inhibition prevents cells from:____________.
a. the harmful effects of enzyme activation by covalent modification of unneeded enzymes.
b. making products that are not needed by inhibiting the activity of enzymes in biosynthetic pathways allosterically.
c. irreversibly inhibiting critical enzymes.
d. destroying enzymes by proteolytic cleavage when they are needed in biosynthetic pathways.
e. accumulating unnecessary proteins.

Answers

Answer:

b. making products that are not needed by inhibiting the activity of enzymes in biosynthetic pathways allosterically.

Explanation:

The feedback inhibition is a method of cellular control were the active cellular enzymes is inhabited and it allows the cells to regulate how enzymes are needed to produce at the end.  This feedback acts as a first enzyme amino acid production and is usually complete through the allosteric site. It changes the shapes and behavior of the active cell.

(Q030) All members of the nuclear receptor family Group of answer choices are cell-surface receptors. do not undergo conformational changes. are found only in the cytoplasm. interact with signal molecules that diffuse through the plasma membrane.

Answers

Answer:

interact with signal molecules that diffuse through the plasma membrane.

Explanation:

Nuclear receptors are responsible for recognizing steroids and other molecules. They interact with signal molecules that diffuse through the plasma membrane.

What are signal molecules?

Signal molecules are responsible for transmission of information. Different signaling molecules are of different size, shapes, confirmations, etc.

Its example include acetylcholine, dopamine, epinephrine, glycine, etc.

These molecules interacts with nuclear receptors and helps in signal transmission to and fro from the cell.

Thus, option d is correct.

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Confirm if all of these are correct for 10 points + brainliest. If they are incorrect, please correct it.

Answers

Answer:

Correct

I am not sure.

Explanation:

Hope it helps you...

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