What are three methods for detecting radioactively labeled DNA in an experiment?

Answers

Answer 1

Answer:

Limitations. detect low abundance DNA binding proteins from lysates. test binding site mutations using many probe configurations with the same lysate. test binding affinity through DNA probe mutational analysis. non-radioactive EMSA possible using biotinylated or fluorescently labeled DNA probes.

Explanation:

Answer 2

Answer:

Proteins interact with DNA through electrostatic interactions (salt bridges), dipolar interactions (hydrogen bonding, H-bonds), entropic effects (hydrophobic interactions) and dispersion forces (base stacking). These forces contribute in varying degrees to proteins binding in a sequence-specific or non–sequence-specific manner.

Explanation:


Related Questions

Koalas live almost entirely on leaves from eucalyptus trees and they are native exclusively to Australia.
Destruction of the Koalas' habitat will most likely lead to -
F)) koalas producing more
offspring
G)) a decrease of the koala
population
H)) the koalas becoming larger.
J)) an increase of the koala
population.

Answers

Answer:

G

Explanation:

it will ruin the koala homes

There is a decrease of the koala population

Mitochondria require glucose to convert ADP to ATP. Which of the following statements describes how ADP is converted into ATP?

A. ADP loses a phosphate group which stores energy for use by the cell.
B. ADP gains a phosphate group which stores energy for use by the cell.
C. ADP loses a phosphate group which releases energy for use by the cell.
D. ADP gains a phosphate group which releases energy for use by the cell.

Answers

Answer:

B. ADP gains a phosphate group which stores energy for use by the cell.

Explanation:

ADP is converted to ATP for the storing of energy by the addition of a high-energy phosphate group. The conversion takes place in the substance between the cell membrane and the nucleus, known as the cytoplasm, or in special energy-producing structures called mitochondria.

The statement that describes how ADP is converted into ATP is as follows:

ADP gains a phosphate group which stores energy for use by the cell.

Thus, the correct option is B.

What is ATP?

ATP stands for Adenosine triphosphate. It may be defined as an organic compound that delivers energy to operate many processes in living cells, such as condensate dissolution, muscle contraction, nerve impulse propagation, and chemical synthesis.

ATP is considered a high-energy compound that furnishes immediate energy to the cell for metabolic activities.

ADP gets converted into ATP by gaining one phosphate group which is the most potent high-energy phosphate. This conversion of ADP to ATP generally takes place in the cytoplasm or in the membrane-bound cell organelle known as mitochondria.

Therefore, the correct option for this question is B.

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Use Demetri's results to explain how temperature affects enzymes

Answers

Answer:

As with many chemical reactions, the rate of an enzyme-catalysed reaction increases as the temperature increases. However, at high temperatures the rate decreases again because the enzyme becomes denatured and can no longer function. ... As the temperature increases so does the rate of enzyme activity.

Explanation:

is centrioles a plant or animal cell?​

Answers

Answer:Animal cells but some plant cells also have it.

Explanation:

write essay about connective tissue?

please write correct answer

Answers

How long do you need the essay to be? 3-5 paragraphs or?

When a food handler can effectively remove soil from equipment using normal methods, the equipment is considered what?

Answers

Answer:

Cleanable

Explanation:

Cleanability refers to the ease with which microorganisms or particles can be removed from the equipment used in food production. To be cleanable the roughness of the machine being employed in production is not expected to exceed 0.8μm.

The Atomic Force Microscope is used to measure the surface roughness of machines. Cleanability is important as it ensures that the machines used in food production are safe.

When a food handler can effectively remove soil from an equipment by using normal methods, the equipment is considered to be; Corrosion Resistant.

The soil referred to her would be dirt because we are talking about a food handler.

Now, if the food handler is finding it difficult to remove dirt from the equipment by using normal/regular methods, it means that the soil/dirt has succeeded in having some kind of chemical reaction with that equipment.

If that is the case, then it means the equipment is undergoing corrosion because corrosion is defined as the gradual destruction of a metal by chemical reaction with the environment.

Since we have established that inability to use normal method denotes corrosion, then if he can use normal methods to remove the soil effectively, then the equipment is said to be corrosion resistant.

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which is not an example of a molecule?
a)NCI3
b)O3
c)H2O2
d) F

Answers

The answer for that is C
I believe the answer is c

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A diagram showing two plates colliding, with one plate moving below the other. What type of plate boundary is illustrated in the image? What is the movement of one plate below another called?

Answers

Answer:

Hello There!! :D

Explanation:

Your answers are:

1. Convergent

2. Subduction

Hope this helps you!! ♥️♥️♥

- abakugosimp

Answer:

A. Convergent

B. Subduction

Explanation:

What type of plate boundary is illustrated in the image?

convergent

What is the movement of one plate below another called?

subduction

Which type of fossils is MOST helpfu
in understanding what the climate w
like for a region in the past?
A.fossils of plants
B. fossils of microorganisms
C. fossils of birds
D. fossils of animals

Answers

Answer:

I would say A). The animals in an area can fluctuate and diversify, while plants can't move and will die if conditions are too intense, leaving only the ones accustomed to the climate to survive.

What happens when the nucleotides in a DNA synthesis reaction are radioactive?

Answers

If a cell is grown in a medium containing radioactively labeled thymidine--which is one of the nucleotides needed to synthesize DNA--after the first round of replication, all of the DNA molecules will have one labeled strand and one unlabeled strand. Why? Because the radioactively labeled thymidine will be incorporated into the newly synthesized strands. If the cell undergoes ANOTHER round of replication in the same medium, the same sequence of events will occur.

Which statement best describes what happens during translation?

Answers

Answer:

B

Explanation:

The entire process is called gene expression. In translation, messenger RNA (mRNA) is decoded in a ribosome, outside the nucleus, to produce a specific amino acid chain, or polypeptide. The polypeptide later folds into an active protein and performs its functions in the cell.

During translation, the ribosome reads the mRNA and uses the information to assemble a protein.

Translation is the second step of protein synthesis. It takes place in the ribosome, a complex structure made up of proteins and RNA. The ribosome reads the mRNA, a molecule that contains the genetic information for a protein. The mRNA is translated into a protein by a process called codon-anticodon pairing.

Codons are three-base sequences in mRNA that code for specific amino acids. Anticodons are complementary sequences in tRNA molecules that bind to specific codons. When the ribosome encounters a codon in the mRNA, it brings in the corresponding tRNA molecule. The tRNA molecule carries the amino acid that corresponds to the codon.

The amino acid is then added to the growing polypeptide chain. The ribosome continues to move down the mRNA, reading codons and adding amino acids until the entire protein is synthesized.

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The temperature of the interior of the human body is about 37oC (98.6oF), regardless of the air temperature. Explain the importance of constant body temperature given the role of enzymes as catalysts in the body.

Answers

It’s very important because let’s say you’re outside on a hot summer day and you’re sweating a lot, sweat would be to help you cool down but you would be losing water in the process. If you stop sweating but you’re still hot you would sweat out and this would cause a heatstroke

Hope this helps

It is critical that we sustain an optimal temperature for our body's enzymes. When it gets too hot, our enzymes denature and become inactive/lost their function. If the temperature is too low, the enzymes will become ineffective.

What is homeostasis?

Homeostasis is the state of constant internal, physical, and chemical assumption is supported by living systems in biology.

This is the state of the organism's optimal functioning, and it includes many variables, such as core temperature as well as fluid balance, getting kept within certain pre-set limits.

Enzymes can only function when the body's temperature remains constant. As a result, homeostasis becomes an important part of the body's metabolism.

Homeostasis maintains optimal conditions for enzyme action and all cell functions throughout the body.

It is the preservation of a constant internal environment in the face of changing internal and external conditions. These include blood glucose concentration control in the human body.

Thus, having an optimum temperature is essential in functioning of enzymes.

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Tropical islands have consistently warm climates because
they are near the equator.
they are popular tourist destinations.
they are surrounded by cool currents.
islands always have warmer climates than large land masses.

Answers

Answer:

they are near the equator

Explanation:

closest to the sun

what makes up cell membranes and is used to create hormones.
A. cholesterol
B. hydrocarbon
C. starch
D. glucose

Answers

Answer:

What makes up cell membranes and is used to create hormones?

cholesterol**

All biomolecules have the following traits except

nitrogen elements**

What enzyme breaks down starch in your saliva?

amylase**

Which soil-based compounds do plants use to create amino acids from glucose?

nitrogen**

What is a lipid that makes up cell membranes and is used to create hormones?

cholesterol**

Cholesterol makes up cell membranes and is used to create hormones.

Cholesterol is a necessary lipid for the body as it contributes to the formation of cell membranes and hormones. However, high amounts of cholesterol in the blood have harmful consequences for health.

Cholesterol has different functions in the body such as:

- Formation of structures: It is one of the main components of the plasma membranes of animal cells.

- Contributes to the formation of hormones: sex hormones (progesterone, estrogens, and testosterone), corticosteroid hormones (cortisol and aldosterone).

- Contributes to the metabolization of calcium to obtain vitamin D.

Additionally, the options hydrocarbon (natural chemical compound obtained from the earth to be used as fuel), starch (natural macromolecule that contributes to the human digestion process) and glucose (the most abundant chemical compound in nature that serves as a source of energy for organisms) are not correct options, because these substances do not have the function of forming cell membranes and creating hormones.

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what are the building blocks of muscle and it provides 4 calories for every gram

Answers

Answer:

Protein

Explanation:

Protein is made up of amino acids that help to grow, maintain and repair different tissues in the body- including muscle.

Answer: The answer is protein

Explanation: I got it right on my test

The Tissues of vascular Plants are organized into more complex structures that carry out a Variety of functions in the plant what structure is formed by a Group of tissues that work together to carry out a Specific function

A) Cell
B) Organ system
C)Organism
D Organ


Due in 19 Minutes Best answer gets Brainliest

Answers

Hi guys sorry to say I don’t know how much you guys do but I wanna

Shameeka is studying for an exam. She took the notes below about calcium and chlorine, which are known to form ionic bonds.

Calcium, which is a metal, donates 2 electrons to chlorine.
The individual calcium atoms become ions with a negative 2 charge.
The bond between calcium and chlorine makes the atoms stable.
Calcium and chlorine attract each other.

Which best describes Shameeka’s error?

Calcium is a metal, so it donates protons, not electrons.
The individual calcium atom has a positive, not negative, 2 charge.
The bond formed makes the atoms unstable, not stable.
Calcium and chlorine repel, not attract, each other.

Answers

Answer:

The individual calcium atom has a positive, not negative, 2 charge.

Explanation:

The error in her study is in the second line of her note which suggests that the individual calcium atoms becomes ions with a negative 2 charge.

When atom loses electrons, it has a net positive charge on it. Not a negative charge. This is because the number of protons is now more than electrons. Therefore, a net charge on the calcium atom will be a positive one. This is her mistake.

Answer:

B) The individual calcium atom has a positive, not negative, 2 charge.

Explanation:

Hope this helps. Have a good day!

Open Response 2 part A: Plant cells and fungal cells have many of the
same types of organelles. Structures X and Y are found in both plant cells
and fungal cells. Structure Z is found in plant cells, but not in fungal cells. A
- What is party?
X
Z

Answers

The answer will be z because u can dance and take the L on black kids

Which pathogen is most likely responsible for causing hoof-and-mouth disease on livestock?

Answers

Answer:

D

Explanation:

A virus, because it requires a host cell to reproduce

2. What is the complementary DNA sequence for the following DNA strands? Write the sequence of the complementary strand below the sequence
given to you.
CGTATC
CTTTCAAG
GAGACTTAC

Answers

Answer:

CGTATC - DNA complementary strand- GCATG

CTTTCAAG- DNA complementary strand-GAAAGTTG

GAGACTTAC-DNA complementary strand-CTCTGAATG

Explanation:

Remember that the complementary sequence will be based on base pairing for DNA, so the A will pair with T, C with G

Which two structures produce energy that cells can
use?
O A and B
OB and C
O Cand D
O D and A

Answers

Answer:

According to the plant cell scheme, the two structures that produce energy that cells can use are D, which corresponds to mitochondria, and A, which is a chloroplast, so the answer is D and A (fourth option).

Explanation:

In the plant cell —unlike the animal cell— there are organelles called chloroplasts, responsible for converting light energy from the sun into chemical energy. In addition, there are mitochondria, which also provide useful energy to the cell.

In the chloroplast, photosynthesis takes place, a process that during the light phase produces ATP and NADPH⁺ from solar energy. In the mitochondria, energy in the form of ATP is obtained from glucose, due to oxidative phosphorylation and the electron transport chain.

The organelles indicated in the diagram are mitochondria (A) and chloroplast (D), both responsible for producing energy for the plant cell.

Answer: D and A

Explanation:

Graves’ Disease is an autoimmune disorder that causes an overproduction of the hormone produced in the thyroid. These hormones are responsible for cellular metabolism. What would this disorder probably result in?

Answers

Answer:

Explanation:

Graves' disease is an autoimmune disorder in which antibodies produced by your immune system stimulate your thyroid to produce too much T4. It's the most common cause of hyperthyroidism. Hyperfunctioning thyroid nodules (toxic adenoma, toxic multinodular goiter or Plummer's disease).

What is one of the roles of the enzyme, DNA Polymerase, during DNA replication?
Answer
F
It degenerates (breaks down) the old DNA strand once the new strand is made
G
It uses a strand of DNA to build a new strand of RNA
H It joins nucleotides to synthesize a complementary strand of DNA
J
It unzips the two strands in a molecule of DNA to begin replication

Answers

Answer:

H

Explanation:

Couldn't find my notes for this, but research says H- it builds a complimentary DNA strand

please help i will give brainist when i can :)

Answers

Answer:

the answer is III.

Explanation:

.OwO.

Hope this helps

An organism that has different alleles - one dominate and one recessive it's called

Answers

Answer:

An organism which has two different alleles of the gene is called heterozygous. Phenotypes (the expressed characteristics) associated with a certain allele can sometimes be dominant or recessive, but often they are neither.

Explanation:

What is natural breeding?

Answers

Answer:

Natural breeding refers to the random mating that occurs between two individuals of the same species. Conversely, selective breeding has been performed for centuries by humans hoping to produce offspring with desirable traits.

what supports and protects the chest cavity?

Answers

Answer: The bones of the chest — namely the rib cage and spine — protect vital organs from injury, and also provide structural support for the body.

Explanation:

What important gas do we take in when we breathe

Answers

Answer:

oxygen

Explanation:

we breath in oxygen and release carbon dioxide

hope this helps :)

A cell has 10% glucose outside and 2% salt inside. Describe the direction of osmosis.
1.Water will move out of the cell.
2.There will be an equal amount of water movement into and out of the cell.
3.Water will move into the cell

Answers

Answer:

1. Water will move out of the cell

- There is a higher concentration outside of the cell

15. What is meant by combustion?​

Answers

Answer: D.

Explanation: Please give me Brainlest Please

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